BASED ON QUARTZ CRYSTAL MICROBALANCE

Serap DURKUT; Şükran ŞEKER

doi:https://doi.org/10.1501/Commub_0000000554

Article Details

BASED ON QUARTZ CRYSTAL MICROBALANCE

Authors:

Serap DURKUT | Şükran ŞEKER

Publisher:

Ankara University

Journal:

Communications Faculty of Sciences University of Ankara Series B Chemistry and Chemical Engineering 2016 Vol.58 Issue 1.2, pp.9-17

Document Type:

Article

Publication Date:

5/2016

Document Language:

English

Document ID (Org No.):

2000709939

Frequency:

Bi-Annual

eISSN:

2687-4806

pISSN:

1303-6017

DOI:

https://doi.org/10.1501/Commub_0000000554

Abstract:

 

In this study, a genosensor based on quartz crystal microbalance  QCM  has been developed   for the detection of single-stranded oligonucleotide specific to alpha-fetoprotein  AFP  gene.   The quartz crystals were coated with polyethyleneimine  PEI  and then crosslinked with   glutaraldehyde  GA . The oligonucleotide probe was immobilized onto the PEI-GA film. The   coatings were characterized by using atomic force microscopy. Hybridization reaction between   probe and the complementary strand was monitored by the QCM system in real-time. This work   demonstrated that QCM is a suitable tool for the real-time monitoring of hybridization on the   crystal surface without using any label.

 

Bu çalışmada, alfa-fetoprotein  AFP  genine özgü tek zincirli oligonükleotit dizisinin   belirlenmesine yönelik olarak geliştirilen, kuartz kristal mikrobalans  KKM    sistemine dayalı bir gen sensörü anlatılmıştır. Kuartz kristaller, poli etilenimin   PEI    polimeri ile kaplanmış, ardından glutaraldehit  GA  ile çapraz bağlama işlemi   gerçekleştirilmiştir. Oligonükleotit probu, hazırlanan PEI/GA yüzeyi üzerine   immobilize edilmiştir. Yüzeylerin kaplanması atomik kuvvet mikroskobu ile   doğrulanmıştır. Tamamlayıcı ve prob dizileri arasında gerçekleşen hibridizasyon   reaksiyonu, KKM sistemi ile eş-zamanlı olarak görüntülenmiştir. Bu çalışma ile,   KKM sensörünün, kristal yüzeyi üzerinde gerçekleşen hibridizasyon reaksiyonunu,   herhangi bir işaretleyici kullanmadan, eş-zamanlı olarak belirleyebildiği   gösterilmiştir.

Article Details

BASED ON QUARTZ CRYSTAL MICROBALANCE

Authors: Serap DURKUT | Şükran ŞEKER

Publisher: Ankara University

Journal: Communications Faculty of Sciences University of Ankara Series B Chemistry and Chemical Engineering 2016 Vol.58 Issue 1.2, pp.9-17

Document Type: Article

Publication Date: 5/2016

Document Language: English

Document ID (Org No.): 2000709939

Frequency: Bi-Annual

eISSN: 2687-4806

pISSN: 1303-6017

DOI: https://doi.org/10.1501/Commub_0000000554

Issues

Authors:

Serap DURKUT | Şükran ŞEKER

Publisher:

Ankara University

Journal:

Communications Faculty of Sciences University of Ankara Series B Chemistry and Chemical Engineering 2016 Vol.58 Issue 1.2, pp.9-17

Document Type:

Article

Publication Date:

5/2016

Document Language:

English

Document ID (Org No.):

2000709939

Frequency:

Bi-Annual

eISSN:

2687-4806

pISSN:

1303-6017

DOI:

https://doi.org/10.1501/Commub_0000000554