EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.

Masara  D. J.; S. I. Neamah

doi:10.36103/ijas.v55iSpecial.1891

Article Details

EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.

Authors:

Masara D. J. | S. I. Neamah

Publisher:

University of Baghdad

Journal:

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.55 Issue Special, pp.111-121

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001372287

pISSN:

2410-0862

DOI:

10.36103/ijas.v55iSpecial.1891

Keywords:

Abstract:

 This study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at a concentration of 1.0, 2.0 and 3.0 mg l-1 and 6-benzyladenine (BA) at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1, with the aim of studying its effect on callus induction. In comparison, Ag-NPs were used at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1 to study its effect on the production of some secondary compounds. The results showed that the mixture consisting of the culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction and the minimum days to complete callus formation. While, the same media with 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BA recorded the highest dry weight (DW) and relative water content (RWC). The combination B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively. As for the production of SC, the B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin, and flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferol.

Article Details

EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.

Authors:

Masara D. J. | S. I. Neamah

Publisher:

University of Baghdad

Journal:

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.55 Issue Special, pp.111-121

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001372287

pISSN:

2410-0862

DOI:

10.36103/ijas.v55iSpecial.1891

in vitro

nanoparticles

flavonoids

alkaloids

Issues

Article Details

EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.

Authors: Masara D. J. | S. I. Neamah

Publisher: University of Baghdad

Journal: IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.55 Issue Special, pp.111-121

Document Type: Article

Publication Date: 1/2024

Document Language: English

Document ID (Org No.): 2001372287

pISSN: 2410-0862

DOI: 10.36103/ijas.v55iSpecial.1891

in vitro

nanoparticles

flavonoids

alkaloids

Issues

Authors:

Masara D. J. | S. I. Neamah

Publisher:

University of Baghdad

Journal:

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.55 Issue Special, pp.111-121

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001372287

pISSN:

2410-0862

DOI:

10.36103/ijas.v55iSpecial.1891