Detection of invasion gene invA in Salmonella spp. Isolated from slaughtered cattle by PCR method: Arcan A N Al-Zubaidy 1, Afaf Abdulrahman Yousif 2, Mawlood A. A. Al-Graibawi 3, Jalil Darkhan 4

Mawlood A. A. Al-Graibawi

doi:10.30539/iraqijvm.v39i1.210

Article Details

Detection of invasion gene invA in Salmonella spp. Isolated from slaughtered cattle by PCR method: Arcan A N Al-Zubaidy 1, Afaf Abdulrahman Yousif 2, Mawlood A. A. Al-Graibawi 3, Jalil Darkhan 4

Authors:

Mawlood A. A. Al-Graibawi

Publisher:

University of Baghdad

Journal:

The Iraqi Journal of Veterinary Medicine Vol.39 Issue 1, pp.128-133

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

2001376331

pISSN:

2410-7409

DOI:

10.30539/iraqijvm.v39i1.210

Keywords:

Abstract:

  The present study was carried out for the identification and molecular characterization of Salmonella spp. isolated from cattle at abattoir by biochemical, serotyping and virulence gene based polymerase chain reaction (PCR) techniques. Eleven Salmonellawere isolated from cattle at abattoir, these isolates were cultured and biochemically characterized by double checking with a conventional method and by KB011 Hi Salmonella TM identification kit then confirmed by serotyping and testing for detection of the invA virulence gene by PCR by using aSalmonella-specific 506bp invA gene amplicon. The biochemical and serotyping results revealed that the 11 isolatesbelonged to four serotypes, S. enteritidis was the predominant serotype,5 isolates (45.45%) followed by S. newport 3 (27.27%), S. ohio, 2 (18.18%) and S. anatum, 1(9.09%). The PCR technique confirmed that all Salmonella isolates carried the invA gene (DNA amplification showed one distinct band with molecular weight of 506 bp amplified fragment on electrophoresis in agarose gel).The PCR assay described herein was found to be a rapid and simple method to confirm the isolates as Salmonella.

Article Details

Detection of invasion gene invA in Salmonella spp. Isolated from slaughtered cattle by PCR method: Arcan A N Al-Zubaidy 1, Afaf Abdulrahman Yousif 2, Mawlood A. A. Al-Graibawi 3, Jalil Darkhan 4

Authors:

Mawlood A. A. Al-Graibawi

Publisher:

University of Baghdad

Journal:

The Iraqi Journal of Veterinary Medicine Vol.39 Issue 1, pp.128-133

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

2001376331

pISSN:

2410-7409

DOI:

10.30539/iraqijvm.v39i1.210

Salmonella spp

invA gene

KB011 Hi Salmonella TM

Cattle

Issues

Article Details

Detection of invasion gene invA in Salmonella spp. Isolated from slaughtered cattle by PCR method: Arcan A N Al-Zubaidy 1, Afaf Abdulrahman Yousif 2, Mawlood A. A. Al-Graibawi 3, Jalil Darkhan 4

Authors: Mawlood A. A. Al-Graibawi

Publisher: University of Baghdad

Journal: The Iraqi Journal of Veterinary Medicine Vol.39 Issue 1, pp.128-133

Document Type: Article

Publication Date: 4/2015

Document Language: English

Document ID (Org No.): 2001376331

pISSN: 2410-7409

DOI: 10.30539/iraqijvm.v39i1.210

Salmonella spp

invA gene

KB011 Hi Salmonella TM

Cattle

Issues

Authors:

Mawlood A. A. Al-Graibawi

Publisher:

University of Baghdad

Journal:

The Iraqi Journal of Veterinary Medicine Vol.39 Issue 1, pp.128-133

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

2001376331

pISSN:

2410-7409

DOI:

10.30539/iraqijvm.v39i1.210