Dnmt3a and Dnmt3b expressions decrease after cryopreservation and re-transplantation of ovarian tissue

Fatma Uysal; Ferda Topal Celikkan; Çiler Çelik-özenci; Sinan Özkavukçu; Gökhan Akkoyunlu

Article Details

Dnmt3a and Dnmt3b expressions decrease after cryopreservation and re-transplantation of ovarian tissue

Authors:

Fatma Uysal | Ferda Topal Celikkan | Çiler Çelik-özenci | Sinan Özkavukçu | Gökhan Akkoyunlu

Publisher:

Akdeniz University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Akdeniz Tıp Dergisi Vol.9 Issue 1, pp.82-89

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001281182

pISSN:

2687-2781

Keywords:

Abstract:

 Objective DNA methylation includes maintenance and de novo methylation process that are catalyzed by DNA methyltransferase Dnmt enzymes and these enzymes possess crucial roles in methylation of genes associated with the development of ovarian follicles. The aim of this study is to evaluate whether expression of Dnmt3a and Dnmt3b protein levels change after cryopreservation and re-transplantation of ovarian tissue in rats. Methods Four groups were designed as; fresh control FC, frozenthawed FT, fresh re-transplanted T, and frozenthawed and re-transplanted FTT. Slow freezing was used to cryopreserve the ovarian tissue pieces and expression of Dnmt3a and Dnmt3b proteins was assessed by immunohistochemistry. Result Expression of Dnmt3a and Dnmt3b significantly decreased in ovarian follicles in FT, T and FTT groups when compared FC. Conclusion Expression of Dnmt enzymes has been altered both after cryopreservation alone and following re-transplantation cycles which may lead to disturbed DNA methylation processes during rat folliculogenesis.

 Objective DNA methylation includes maintenance and de novo methylation process that are catalyzed by DNA methyltransferase Dnmt enzymes and these enzymes possess crucial roles in methylation of genes associated with the development of ovarian follicles. The aim of this study is to evaluate whether expression of Dnmt3a and Dnmt3b protein levels change after cryopreservation and re-transplantation of ovarian tissue in rats. Methods Four groups were designed as; fresh control FC, frozenthawed FT, fresh re-transplanted T, and frozenthawed and re-transplanted FTT. Slow freezing was used to cryopreserve the ovarian tissue pieces and expression of Dnmt3a and Dnmt3b proteins was assessed by immunohistochemistry. Result Expression of Dnmt3a and Dnmt3b significantly decreased in ovarian follicles in FT, T and FTT groups when compared FC. Conclusion Expression of Dnmt enzymes has been altered both after cryopreservation alone and following re-transplantation cycles which may lead to disturbed DNA methylation processes during rat folliculogenesis.

 Objective DNA methylation includes maintenance and de novo methylation process that are catalyzed by DNA methyltransferase Dnmt enzymes and these enzymes possess crucial roles in methylation of genes associated with the development of ovarian follicles. The aim of this study is to evaluate whether expression of Dnmt3a and Dnmt3b protein levels change after cryopreservation and re-transplantation of ovarian tissue in rats. Methods Four groups were designed as; fresh control FC, frozenthawed FT, fresh re-transplanted T, and frozenthawed and re-transplanted FTT. Slow freezing was used to cryopreserve the ovarian tissue pieces and expression of Dnmt3a and Dnmt3b proteins was assessed by immunohistochemistry. Result Expression of Dnmt3a and Dnmt3b significantly decreased in ovarian follicles in FT, T and FTT groups when compared FC. Conclusion Expression of Dnmt enzymes has been altered both after cryopreservation alone and following re-transplantation cycles which may lead to disturbed DNA methylation processes during rat folliculogenesis.

 Amaç DNA metilasyonu sürdürme ve de novo metilasyon süreçlerini içerir ve DNA metiltransferaz Dnmt enzimleri tarafından katalizlenir, ovaryum foliküllerinin gelişimi ile ilişkili genlerin metilasyonunda önemli role sahiptir. Çalışmamızın amacı, sıçanlarda ovaryum dokusunun kriyoprezervasyonu ve yeniden transplantasyonu sonrasında Dnmt3a ve Dnmt3b protein düzeylerinin ekspresyonunun değişip değişmediğini değerlendirmektir. Yöntemler Kontrol K, donmuşçözdürülmüş DÇ, transplante edilmiş T ve dondurulmuşçözdürülmüş ve yeniden transplante edilmiş DÇT olmak üzere dört grup oluşturuldu. Ovaryum doku parçalarını dondurmak için yavaş dondurma tekniği kullanıldı ve Dnmt3a ve Dnmt3b proteinlerinin ekspresyonu immünohistokimya yöntemi ile değerlendirildi. Result K ile karşılaştırıldığında, DÇ, T ve DÇT gruplarında ovaryum foliküllerinde Dnmt3a ve Dnmt3b ekspresyonu önemli ölçüde azaldı. Sonuç Hem kriyoprezervasyon hem de yeniden transplantasyondan sonra değişen Dnmt enzimlerinin ekspresyonu sıçan folikülogenezi sırasında DNA metilasyonu süreçlerinin bozulması ile ilişkili olabilir.

Article Details

Dnmt3a and Dnmt3b expressions decrease after cryopreservation and re-transplantation of ovarian tissue

Authors:

Fatma Uysal | Ferda Topal Celikkan | Çiler Çelik-özenci | Sinan Özkavukçu | Gökhan Akkoyunlu

Publisher:

Akdeniz University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Akdeniz Tıp Dergisi Vol.9 Issue 1, pp.82-89

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001281182

pISSN:

2687-2781

DNA methylation

Dnmt enzymes

ovarian tissue cryopreservation

fertility preservation

DNA metilasyonu

Dnmt enzimleri

ovaryum doku kriyoprezevasyonu

fertilitenin korunması

Issues

Article Details

Authors: Fatma Uysal | Ferda Topal Celikkan | Çiler Çelik-özenci | Sinan Özkavukçu | Gökhan Akkoyunlu

Publisher: Akdeniz University

Main Topics: Klinik Tıp Bilimleri

Journal: Akdeniz Tıp Dergisi Vol.9 Issue 1, pp.82-89

Document Type: Article

Publication Date: 1/2023

Document Language: Turkish

Document ID (Org No.): 2001281182

pISSN: 2687-2781

Issues

Authors:

Fatma Uysal | Ferda Topal Celikkan | Çiler Çelik-özenci | Sinan Özkavukçu | Gökhan Akkoyunlu

Publisher:

Akdeniz University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Akdeniz Tıp Dergisi Vol.9 Issue 1, pp.82-89

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001281182

pISSN:

2687-2781