Evaluating the bacterial culture technique by Polymerase chain reaction for the diagnosis of Brucella abortus in milk of cows suspected with brucellosis.: Bashar Sadeq Noomy and Nihad Abdulhassan Jafar

Bashar Sadeq Noomy

doi:10.30539/iraqijvm.v40i1.130

Article Details

Evaluating the bacterial culture technique by Polymerase chain reaction for the diagnosis of Brucella abortus in milk of cows suspected with brucellosis.: Bashar Sadeq Noomy and Nihad Abdulhassan Jafar

Authors:

Bashar Sadeq Noomy

Publisher:

University of Baghdad

Journal:

The Iraqi Journal of Veterinary Medicine Vol.40 Issue 1, pp.5-8

Document Type:

Article

Publication Date:

5/2016

Document Language:

English

Document ID (Org No.):

2001376365

pISSN:

2410-7409

DOI:

10.30539/iraqijvm.v40i1.130

Keywords:

Abstract:

  The aim of this study is to determine the sensitivity of bacterial culture technique in the detection of Brucella abortus in milk samples of aborted cows. Sixty samples of milk were collected from aborted cows during a period which did not exceed two months after the abortion. All of them were positive for rose bengal test. Results showed that Brucella abortus was isolated from 7 out of 60 (11.6%) from the milk of aborted cows, while PCR test showed that 32 out of 60 (53.3%) milk sample contained Brucella abortus. The specificity of culture techniques was 10%, but its sensitivity was only 21.8%. Beside the cautions in dealing with live Brucella abortus (as culture), it is also less sensitive than PCR, though it is better to use PCR technique in the diagnosis of brucellosis in aborted cows milk.

Article Details

Evaluating the bacterial culture technique by Polymerase chain reaction for the diagnosis of Brucella abortus in milk of cows suspected with brucellosis.: Bashar Sadeq Noomy and Nihad Abdulhassan Jafar

Authors:

Bashar Sadeq Noomy

Publisher:

University of Baghdad

Journal:

The Iraqi Journal of Veterinary Medicine Vol.40 Issue 1, pp.5-8

Document Type:

Article

Publication Date:

5/2016

Document Language:

English

Document ID (Org No.):

2001376365

pISSN:

2410-7409

DOI:

10.30539/iraqijvm.v40i1.130

Brucella abotrus

Milk

PCR

Cow

Brucellosis

Issues

Article Details

Evaluating the bacterial culture technique by Polymerase chain reaction for the diagnosis of Brucella abortus in milk of cows suspected with brucellosis.: Bashar Sadeq Noomy and Nihad Abdulhassan Jafar

Authors: Bashar Sadeq Noomy

Publisher: University of Baghdad

Journal: The Iraqi Journal of Veterinary Medicine Vol.40 Issue 1, pp.5-8

Document Type: Article

Publication Date: 5/2016

Document Language: English

Document ID (Org No.): 2001376365

pISSN: 2410-7409

DOI: 10.30539/iraqijvm.v40i1.130

Brucella abotrus

Milk

PCR

Cow

Brucellosis

Issues

Authors:

Bashar Sadeq Noomy

Publisher:

University of Baghdad

Journal:

The Iraqi Journal of Veterinary Medicine Vol.40 Issue 1, pp.5-8

Document Type:

Article

Publication Date:

5/2016

Document Language:

English

Document ID (Org No.):

2001376365

pISSN:

2410-7409

DOI:

10.30539/iraqijvm.v40i1.130