Cytotoxicity Evaluation of Different Types of CAD/CAM Blocks

Fikret Özgür Coşkun; Giray Buğra Akbaba; Mustafa Hayati Atala; Semanur Özüdoğru

Article Details

Cytotoxicity Evaluation of Different Types of CAD/CAM Blocks

Authors:

Fikret Özgür Coşkun | Giray Buğra Akbaba | Mustafa Hayati Atala | Semanur Özüdoğru

Publisher:

Selcuk University

Main Topics:

Dentistry

Journal:

Selcuk Dental Journal Vol.10 Issue 2, pp.462-467

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001294043

pISSN:

2148-7529

Keywords:

Abstract:

 Aim: The aim of this study is to determine the cytotoxic effects of Computer Aided Design CAD and Computer Aided Manufacturing CAM blocks produced by different companies on human peripheral blood lymphocytes by MTT assay. Methods: Six different CADCAM materials were investigated: feldspar ceramic VM Vitablocks Mark II , resin nano-ceramic LU Lava Ultimate, hybrid ceramic C Cerasmart, leucite-reinforced ceramic LRF GC LRF, zirconia-reinforced lithium silicate ceramic VS Vita Suprinity, polymer-infiltrated ceramic-network VE Vita Enamic. A total of 36 disc-shaped samples Ø: 5 mm; h: 2 mm were prepared from commercial blanks and blocks. Cell proliferation and cytotoxicity were assessed at 24h and 48h using MTT assay. The data were statistically evaluated with the Two-way ANOVA test p  0.05. Results: MTT viability data at 24 h showed that group VM and LU blocks were mildly cytotoxic, but there was no statistically significant difference when compared with other groups p0.05. It was determined that all blocks caused cell proliferation after 48 hours of exposure p0.05. It has been shown that all blocks whose cytotoxic effects were investigated did not cause any toxic effects except VM and LU for 24 h at different application times 24 h and 48 h. Conclusion: Based on the results obtained and the limitations of the current in vitro study, the tested materials were not cytotoxic. Only VM and LU caused negligible cytotoxicity at 24-hour exposure.

 Aim: The aim of this study is to determine the cytotoxic effects of Computer Aided Design CAD and Computer Aided Manufacturing CAM blocks produced by different companies on human peripheral blood lymphocytes by MTT assay. Methods: Six different CADCAM materials were investigated: feldspar ceramic VM Vitablocks Mark II , resin nano-ceramic LU Lava Ultimate, hybrid ceramic C Cerasmart, leucite-reinforced ceramic LRF GC LRF, zirconia-reinforced lithium silicate ceramic VS Vita Suprinity, polymer-infiltrated ceramic-network VE Vita Enamic. A total of 36 disc-shaped samples Ø: 5 mm; h: 2 mm were prepared from commercial blanks and blocks. Cell proliferation and cytotoxicity were assessed at 24h and 48h using MTT assay. The data were statistically evaluated with the Two-way ANOVA test p  0.05. Results: MTT viability data at 24 h showed that group VM and LU blocks were mildly cytotoxic, but there was no statistically significant difference when compared with other groups p0.05. It was determined that all blocks caused cell proliferation after 48 hours of exposure p0.05. It has been shown that all blocks whose cytotoxic effects were investigated did not cause any toxic effects except VM and LU for 24 h at different application times 24 h and 48 h. Conclusion: Based on the results obtained and the limitations of the current in vitro study, the tested materials were not cytotoxic. Only VM and LU caused negligible cytotoxicity at 24-hour exposure.

 Aim: The aim of this study is to determine the cytotoxic effects of Computer Aided Design CAD and Computer Aided Manufacturing CAM blocks produced by different companies on human peripheral blood lymphocytes by MTT assay. Methods: Six different CADCAM materials were investigated: feldspar ceramic VM Vitablocks Mark II , resin nano-ceramic LU Lava Ultimate, hybrid ceramic C Cerasmart, leucite-reinforced ceramic LRF GC LRF, zirconia-reinforced lithium silicate ceramic VS Vita Suprinity, polymer-infiltrated ceramic-network VE Vita Enamic. A total of 36 disc-shaped samples Ø: 5 mm; h: 2 mm were prepared from commercial blanks and blocks. Cell proliferation and cytotoxicity were assessed at 24h and 48h using MTT assay. The data were statistically evaluated with the Two-way ANOVA test p  0.05. Results: MTT viability data at 24 h showed that group VM and LU blocks were mildly cytotoxic, but there was no statistically significant difference when compared with other groups p0.05. It was determined that all blocks caused cell proliferation after 48 hours of exposure p0.05. It has been shown that all blocks whose cytotoxic effects were investigated did not cause any toxic effects except VM and LU for 24 h at different application times 24 h and 48 h. Conclusion: Based on the results obtained and the limitations of the current in vitro study, the tested materials were not cytotoxic. Only VM and LU caused negligible cytotoxicity at 24-hour exposure.

 Amaç: Bu çalışmanın amacı, farklı firmalar tarafından üretilen Computer Aided Design CAD ve Computer Aided Manufacturing CAM bloklarının insan periferik kan lenfositleri üzerindeki sitotoksik etkilerini MTT testi ile belirlemektir. Yöntem: Altı farklı CADCAM materyali araştırıldı: feldspat seramik VM Vitablocks Mark II, rezin esaslı nano-seramik LU Lava Ultimate, hibrit seramik C Cerasmart, lösitle güçlendirilmiş cam seramik LRF GC LRF, zirkonya ile güçlendirilmiş lityum silikat seramik VS Vita Suprinity, polimer infiltrasyonu ile güçlendirilmiş cam seramik VE Vita Enamic. CADCAM bloklardan toplam 36 disk şeklinde numune Ø: 5 mm; h: 2 mm hazırlandı. Hücre proliferasyonu ve sitotoksisite, MTT testi kullanılarak 24. ve 48. saatlerde değerlendirildi. Veriler İki Yönlü ANOVA testi ile istatistiksel olarak değerlendirildi p  0.05. Bulgular: 24 saatteki MTT canlılık verileri, grup VM ve LU bloklarının hafif sitotoksik olduğunu gösterdi, ancak diğer gruplarla karşılaştırıldığında istatistiksel olarak anlamlı bir fark yoktu p0.05. Tüm blokların 48 saat sonra hücre çoğalmasına neden olduğu belirlendi p0.05. Sitotoksik etkileri araştırılan tüm blokların farklı uygulama sürelerinde 24 saat ve 48 saat herhangi bir toksik etkiye 24 saat VM ve LU hariç neden olmadığı gösterildi. Sonuç: Elde edilen sonuçlara ve mevcut in vitro çalışmanın sınırlamalarına dayanarak, test edilen materyaller sitotoksik değildi. 24 saatlik sonuçlarda VM ve LU ihmal edilebilir sitotoksisiteye neden oldu.

Article Details

Cytotoxicity Evaluation of Different Types of CAD/CAM Blocks

Authors:

Fikret Özgür Coşkun | Giray Buğra Akbaba | Mustafa Hayati Atala | Semanur Özüdoğru

Publisher:

Selcuk University

Main Topics:

Dentistry

Journal:

Selcuk Dental Journal Vol.10 Issue 2, pp.462-467

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001294043

pISSN:

2148-7529

human peripheral lymphocytes

cytotoxicity

CAD/CAM blocksinsan periferik lenfositleri

sitotoksisite

CAD/CAM blokları

Issues

Article Details

Cytotoxicity Evaluation of Different Types of CAD/CAM Blocks

Authors: Fikret Özgür Coşkun | Giray Buğra Akbaba | Mustafa Hayati Atala | Semanur Özüdoğru

Publisher: Selcuk University

Main Topics: Dentistry

Journal: Selcuk Dental Journal Vol.10 Issue 2, pp.462-467

Document Type: Article

Publication Date: 8/2023

Document Language: English

Document ID (Org No.): 2001294043

pISSN: 2148-7529

human peripheral lymphocytes

cytotoxicity

CAD/CAM blocksinsan periferik lenfositleri

sitotoksisite

CAD/CAM blokları

Issues

Authors:

Fikret Özgür Coşkun | Giray Buğra Akbaba | Mustafa Hayati Atala | Semanur Özüdoğru

Publisher:

Selcuk University

Main Topics:

Dentistry

Journal:

Selcuk Dental Journal Vol.10 Issue 2, pp.462-467

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001294043

pISSN:

2148-7529