Evaluation of UCA1/miR-138/CDK6 Network in the Patients with Laryngeal Squamous Cell Carcinoma

Neslihan Cinkara; Çiğdem Yüce Kahraman; Arzu Tatar; Abdulgani Tatar

Article Details

Evaluation of UCA1/miR-138/CDK6 Network in the Patients with Laryngeal Squamous Cell Carcinoma

Authors:

Neslihan Cinkara | Çiğdem Yüce Kahraman | Arzu Tatar | Abdulgani Tatar

Publisher:

Selcuk University

Main Topics:

İç Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Journal:

Genel Tıp Dergisi Vol.34 Issue 5, pp.666-670

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001330473

pISSN:

2602-3741

Keywords:

Abstract:

 Objective: Noncoding RNAs ncRNAs have the potential to be diagnostic and therapeutic targets, in many cancer types. miRNA  mRNA ceRNA competing endogenous RNA network in cancer has been an interesting topic that has been studied in recent years. We aimed to investigate the relationship of UCA1miR-138CDK6 network with laryngeal squamous cell carcinoma LSCC. Material and Methods: We studied with the cancerous tissue and as the control; neighboring normal tissue samples of the patients with diagnosis of LSCC. UCA1, miR-138 and CDK6 expression analysis was made by quantitative real-time PCR qRT-PCR method after RNA isolation of the samples. Results: Expressions of cancerous tissue samples and normal adjacent tissue samples as controls were compared. CDK6 and UCA1 levels were increased and miR-138 levels were found to be decreased in cancer tissues but not statistically significant. Conclusions: To the best of our knowledge there is no study to provide an expression profile of the UCA1CDK6miR-138 network for LSCC patients. Although we could not obtain statistically significant results, our results were similar with the UCA1, miR138, and CDK6 axis literature. Further studies with larger patient samples and LSCC cell lines may confirm the function of this axis, which might be a diagnostic and therapeutic target for LSCC.

 Objective: Noncoding RNAs ncRNAs have the potential to be diagnostic and therapeutic targets, in many cancer types. miRNA  mRNA ceRNA competing endogenous RNA network in cancer has been an interesting topic that has been studied in recent years. We aimed to investigate the relationship of UCA1miR-138CDK6 network with laryngeal squamous cell carcinoma LSCC. Material and Methods: We studied with the cancerous tissue and as the control; neighboring normal tissue samples of the patients with diagnosis of LSCC. UCA1, miR-138 and CDK6 expression analysis was made by quantitative real-time PCR qRT-PCR method after RNA isolation of the samples. Results: Expressions of cancerous tissue samples and normal adjacent tissue samples as controls were compared. CDK6 and UCA1 levels were increased and miR-138 levels were found to be decreased in cancer tissues but not statistically significant. Conclusions: To the best of our knowledge there is no study to provide an expression profile of the UCA1CDK6miR-138 network for LSCC patients. Although we could not obtain statistically significant results, our results were similar with the UCA1, miR138, and CDK6 axis literature. Further studies with larger patient samples and LSCC cell lines may confirm the function of this axis, which might be a diagnostic and therapeutic target for LSCC.

 Objective: Noncoding RNAs ncRNAs have the potential to be diagnostic and therapeutic targets, in many cancer types. miRNA  mRNA ceRNA competing endogenous RNA network in cancer has been an interesting topic that has been studied in recent years. We aimed to investigate the relationship of UCA1miR-138CDK6 network with laryngeal squamous cell carcinoma LSCC. Material and Methods: We studied with the cancerous tissue and as the control; neighboring normal tissue samples of the patients with diagnosis of LSCC. UCA1, miR-138 and CDK6 expression analysis was made by quantitative real-time PCR qRT-PCR method after RNA isolation of the samples. Results: Expressions of cancerous tissue samples and normal adjacent tissue samples as controls were compared. CDK6 and UCA1 levels were increased and miR-138 levels were found to be decreased in cancer tissues but not statistically significant. Conclusions: To the best of our knowledge there is no study to provide an expression profile of the UCA1CDK6miR-138 network for LSCC patients. Although we could not obtain statistically significant results, our results were similar with the UCA1, miR138, and CDK6 axis literature. Further studies with larger patient samples and LSCC cell lines may confirm the function of this axis, which might be a diagnostic and therapeutic target for LSCC.

 Amaç: Kodlamayan RNAlar ncRNAlar, birçok kanser türünde tanı ve tedavi hedefi olma potansiyeline sahiptir. miRNA  mRNA ceRNA yarışmalı endojen RNA ağı son yıllarda üzerinde çalışılan ilginç bir konu olmuştur. Bu çalışmayla UCA1miR-138CDK6 ağının laringeal skuamöz hücreli karsinom LSCC ile ilişkisini araştırmayı amaçladık. Gereç ve Yöntem: LSCC tanısı alan hastaların kanserli dokuları ve kontrol olarak da komşu normal doku örneklerinden çalışma yapıldı. Örneklerden RNA izolasyonu yapıldıktan sonra kantitatif gerçek zamanlı PCR qRT-PCR yöntemi ile UCA1, miR-138 ve CDK6 ekspresyonları değerlendirildi. Bulgular: Kanserli ve kontrol doku örneklerinin ekspresyonları karşılaştırıldı. Kanser dokularında, istatistiksel olarak anlamlı olmamakla birlikte CDK6 ve UCA1 ekspresyonunun arttığı, miR-138 ekspresyonun ise azaldığı belirlendi. Sonuç: Çalışmamız, bildiğimiz kadarıyla LSCC hastalarında UCA1CDK6miR-138 ağı ekspresyon profilinin çalışıldığı ilk çalışmadır. İstatistiksel olarak anlamlı sonuçlar elde edilememiş olmakla birlikte, sonuçlarımız UCA1, miR-138 ve CDK6 ağ çalışma literatürüyle benzerdi. Örnek sayısının daha fazla olduğu çalışmalar ve LSCC hücre dizileri ile yapılacak ileri çalışmalar, LSCC için tanı ve tedavi hedefi olabilecek bu yolağın fonksiyonlarını daha da netleştirecektir.

Article Details

Evaluation of UCA1/miR-138/CDK6 Network in the Patients with Laryngeal Squamous Cell Carcinoma

Authors:

Neslihan Cinkara | Çiğdem Yüce Kahraman | Arzu Tatar | Abdulgani Tatar

Publisher:

Selcuk University

Main Topics:

İç Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Journal:

Genel Tıp Dergisi Vol.34 Issue 5, pp.666-670

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001330473

pISSN:

2602-3741

Long non-coding RNA

lncRNA

Laryngeal cancer

miRNA

UCA1

miR138

CDK6Uzun kodlamayan RNA

lncRNA

Laringeal kanser

miRNA

UCA1

miR138

CDK6

Issues

Article Details

Authors: Neslihan Cinkara | Çiğdem Yüce Kahraman | Arzu Tatar | Abdulgani Tatar

Publisher: Selcuk University

Main Topics: İç Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Journal: Genel Tıp Dergisi Vol.34 Issue 5, pp.666-670

Document Type: Article

Publication Date: 10/2024

Document Language: English

Document ID (Org No.): 2001330473

pISSN: 2602-3741

Issues

Authors:

Neslihan Cinkara | Çiğdem Yüce Kahraman | Arzu Tatar | Abdulgani Tatar

Publisher:

Selcuk University

Main Topics:

İç Hastalıkları | Tıbbi Genetik (Kanser Genetiği hariç)

Journal:

Genel Tıp Dergisi Vol.34 Issue 5, pp.666-670

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001330473

pISSN:

2602-3741