Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme

Rafika Mila Amanah; Dita Prameswari Trenggono Putri; Mohammad Zainal Abidin; Yuny Erwanto; Yuny Erwanto

doi:10.3844/ajavsp.2025.97.102

Article Details

Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme

Authors:

Rafika Mila Amanah | Dita Prameswari Trenggono Putri | Mohammad Zainal Abidin | Yuny Erwanto | Yuny Erwanto

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.20 Issue 2, pp.97-102

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001396809

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2025.97.102

Keywords:

Abstract:

 The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis (SDS-PAGE). The results showed the highestcollagen yield was 7.77% from D treatment; the viscosity ranges were 2.18-1.99cP, and the pH ranges were 4.89-4.68. FTIR spectra showed amide A, B, I, II,and III. SDS-PAGE results showed three distinct main bands, consisting of α1,α2, and β chains. In conclusion, collagen could be extracted from Local duckfeet and categorized as collagen type I.

Article Details

Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme

Authors:

Rafika Mila Amanah | Dita Prameswari Trenggono Putri | Mohammad Zainal Abidin | Yuny Erwanto | Yuny Erwanto

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.20 Issue 2, pp.97-102

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001396809

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2025.97.102

Duck Feet

Collagen Isolation

Bromelain Enzyme

Characterization

Issues

Article Details

Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme

Authors: Rafika Mila Amanah | Dita Prameswari Trenggono Putri | Mohammad Zainal Abidin | Yuny Erwanto | Yuny Erwanto

Publisher: Science Publications

Journal: American Journal of Animal and Veterinary Sciences Vol.20 Issue 2, pp.97-102

Document Type: Article

Publication Date: 5/2025

Document Language: English

Document ID (Org No.): 2001396809

pISSN: 1557-4563

DOI: 10.3844/ajavsp.2025.97.102

Duck Feet

Collagen Isolation

Bromelain Enzyme

Characterization

Issues

Authors:

Rafika Mila Amanah | Dita Prameswari Trenggono Putri | Mohammad Zainal Abidin | Yuny Erwanto | Yuny Erwanto

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.20 Issue 2, pp.97-102

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001396809

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2025.97.102