RT-PCR Analysis of Caucasian and Mugla Honey Bees by SNP Markers of Chalkbrood Disease

Ahmet Okumuş; Fatih Bilge

Article Details

RT-PCR Analysis of Caucasian and Mugla Honey Bees by SNP Markers of Chalkbrood Disease

Authors:

Ahmet Okumuş | Fatih Bilge

Publisher:

Bolu Abant Izzet Baysal University

Main Topics:

Ziraat | Veterinerlik ve Gıda Bilimleri

Journal:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.196-204

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001293825

pISSN:

2149-8245

Keywords:

Abstract:

 Two different honey bee subspecies genotypes obtained from Ordu, Apiculture Research Institute were analyzed by SNP markers using Real-Time PCR-HRM. Genomic DNA samples analysed with 10 SNP primers those were used for identification of chalkbrood disease resistance genes and two SNP primers those were obtained from honey bee genom sequencing. Result of SNP analyses, four primers AMB-00858574, AMB-01151447, AMB00631190, AMB-00686140 amplified in Caucasian honey bee and six AMB-00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00674355, AMB-00686140 primers amplified in Mugla honey bee. Four amplicons are similar for this subspecies. Result of electrophoresis analyses, five primers AMB00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00686140 form a band in Caucasian honey bee, seven amplicons AMB-00858574, AMB00612262, AMB-01151447, AMB-00631190, AMB-00902548, AMB-00674355, AMB-00686140 form a band in Mugla honey bee and four amplicons AMB00858574, AMB-01151447, AMB-00631190, AMB-00686140 similar for this subspecies. As a result of this study, in Caucasian and Muğla honeybee for identification of disease resistance and evaluability as a identification key for this subspecies was emerged the capacity of association of single nucleotide polymorphisms to resistance to chalkbrood in two important honeybee genotypes in country of Apis mellifera using RT-PCR for HRM analysis.

 Two different honey bee subspecies genotypes obtained from Ordu, Apiculture Research Institute were analyzed by SNP markers using Real-Time PCR-HRM. Genomic DNA samples analysed with 10 SNP primers those were used for identification of chalkbrood disease resistance genes and two SNP primers those were obtained from honey bee genom sequencing. Result of SNP analyses, four primers AMB-00858574, AMB-01151447, AMB00631190, AMB-00686140 amplified in Caucasian honey bee and six AMB-00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00674355, AMB-00686140 primers amplified in Mugla honey bee. Four amplicons are similar for this subspecies. Result of electrophoresis analyses, five primers AMB00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00686140 form a band in Caucasian honey bee, seven amplicons AMB-00858574, AMB00612262, AMB-01151447, AMB-00631190, AMB-00902548, AMB-00674355, AMB-00686140 form a band in Mugla honey bee and four amplicons AMB00858574, AMB-01151447, AMB-00631190, AMB-00686140 similar for this subspecies. As a result of this study, in Caucasian and Muğla honeybee for identification of disease resistance and evaluability as a identification key for this subspecies was emerged the capacity of association of single nucleotide polymorphisms to resistance to chalkbrood in two important honeybee genotypes in country of Apis mellifera using RT-PCR for HRM analysis.

 Two different honey bee subspecies genotypes obtained from Ordu, Apiculture Research Institute were analyzed by SNP markers using Real-Time PCR-HRM. Genomic DNA samples analysed with 10 SNP primers those were used for identification of chalkbrood disease resistance genes and two SNP primers those were obtained from honey bee genom sequencing. Result of SNP analyses, four primers AMB-00858574, AMB-01151447, AMB00631190, AMB-00686140 amplified in Caucasian honey bee and six AMB-00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00674355, AMB-00686140 primers amplified in Mugla honey bee. Four amplicons are similar for this subspecies. Result of electrophoresis analyses, five primers AMB00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00686140 form a band in Caucasian honey bee, seven amplicons AMB-00858574, AMB00612262, AMB-01151447, AMB-00631190, AMB-00902548, AMB-00674355, AMB-00686140 form a band in Mugla honey bee and four amplicons AMB00858574, AMB-01151447, AMB-00631190, AMB-00686140 similar for this subspecies. As a result of this study, in Caucasian and Muğla honeybee for identification of disease resistance and evaluability as a identification key for this subspecies was emerged the capacity of association of single nucleotide polymorphisms to resistance to chalkbrood in two important honeybee genotypes in country of Apis mellifera using RT-PCR for HRM analysis.

 Ordu, Arıcılık Araştırma Enstitüsünden sağlanan iki farklı bal arısı alt türüne ait genotipler Real-Time PCR-HRM kullanılarak SNP markörleri ile analiz edilmiştir. Kireç hastalığı direnç genlerinin tanımlanmasında kullanılan 10 SNP primeri ve bal arısı genom dizilemesinden elde edilen iki SNP primeri ile analiz edilen genomik DNA örneklerinde; Kafkas bal arısında amplifiye edilmiş dört primer AMB-00858574, AMB-01151447, AMB 00631190, AMB-00686140 ve altı AMB-00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00674355, AMB-00686140 Muğla bal arısında amplifiye edilmiştir. Bu alt tür için dört amplikon benzer olup elektroforez analizleri sonucunda, Kafkas bal arısında beş primer AMB 00858574, AMB-00612262, AMB-01151447, AMB-00631190, AMB-00686140, yedi amplikon AMB-00858574, AMB 00612262, AMB-01151447, AMB -00631190, AMB-00902548, AMB-00674355, AMB-00686140 Muğla bal arısında dört amplikon AMB 00858574, AMB-01151447, AMB-00631190, AMB-00686140 bir bant oluşturmuştur. Bu çalışma sonucunda, Kafkas ve Muğla bal arısı genotiplerinde hastalık direncinin belirlenmesi ve bu alt tür için bir tanımlama anahtarı olarak değerlendirilebileceği, HRM analizi için RT-PCR kullanarak Apis melliferanın önemli iki bal arısı genotipinde tek nükleotid polimorfizmlerinin kireç direnci ile ilişkilendirme kapasitesi ortaya çıkarılmıştır.

Article Details

RT-PCR Analysis of Caucasian and Mugla Honey Bees by SNP Markers of Chalkbrood Disease

Authors:

Ahmet Okumuş | Fatih Bilge

Publisher:

Bolu Abant Izzet Baysal University

Main Topics:

Ziraat | Veterinerlik ve Gıda Bilimleri

Journal:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.196-204

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001293825

pISSN:

2149-8245

Honey Bee

SNP

Mugla bee

Caucasian bee

Real-Time PCR

HRM

chalkbrood diseaseBal arısı

Mugla arısı

Kafkas arısı

SNP

RT-PCR

HRM

kireç hastalığı

Issues

Article Details

Authors: Ahmet Okumuş | Fatih Bilge

Publisher: Bolu Abant Izzet Baysal University

Main Topics: Ziraat | Veterinerlik ve Gıda Bilimleri

Journal: Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.196-204

Document Type: Article

Publication Date: 8/2023

Document Language: English

Document ID (Org No.): 2001293825

pISSN: 2149-8245

Issues

Authors:

Ahmet Okumuş | Fatih Bilge

Publisher:

Bolu Abant Izzet Baysal University

Main Topics:

Ziraat | Veterinerlik ve Gıda Bilimleri

Journal:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.196-204

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001293825

pISSN:

2149-8245