Methanolic extract of Eryngium creticum Lam leaves,  flowers and roots: Quantification and qualification of  phenolic contents, antitumor effect and antioxidant  capacity

N.A.

Article Details

Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Clinical Pharmacy and Pharmacy Practice

Journal:

Journal of Research in Pharmacy Vol.26 Issue 4, pp.770-780

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347269

pISSN:

2630-6344

Keywords:

Abstract:

 Natural products are potential sources of prospective antitumor agents. The phenolic and flavonoid contents of leaves, flowers, and roots of Eryngium creticum were quantified using liquid chromatography-tandem mass spectrometry LC-ESI-MSMS. The antioxidant capacity and antitumor activity of the three extracts were tested on MCF-7 and Hep2. The average total phenolic contents of E. creticum leaves, flowers, and roots were 12.43  0.24, 10.09  0.20, and 4.78  0.10 mg of GAEg of dw, respectively. The average total flavonoids were 4.77  0.10, 6.23  0.12, and 5.62  0.12 mg of QEg of dw, respectively. The extracts showed considerable DPPH scavenger activity with average IC50 values of 1.41  0.06, 2.70  0.06, and 3.03  0.05 µgmL, respectively. The average IC50 values of scavenging capacity of NO radicals were 0.11  0.03, 0.69  0.04, and 0.44  0.01 µgmL, respectively. The average IC50 values in MCF-7 were 1.25  0.50, 3.75  0.48, and 1.88  0.28 mgmL, respectively. In Hep2, the average IC50 values were 1.16  0.58, 3.75  0.79, and 1.31  0.47 µgmL, respectively. Phenolic content varied between the different extracts; leaves showed the highest levels of gallic acid 3.60 µgmL, querciti-3-O-galactoside 0.82 µgmL, cyanin chloride 1.52 µgmL, and rosmarinic acid 4.65 µgmL. Roots showed the lowest quantities of the measured phenolics, except for chlorogenic acid 6.00 µgmL and rosmarinic acid 1.86 µgmL. The three extracts of E. creticum possess in vitro antitumor activities, which could be utilized as an adjunct with chemotherapy.

 Natural products are potential sources of prospective antitumor agents. The phenolic and flavonoid contents of leaves, flowers, and roots of Eryngium creticum were quantified using liquid chromatography-tandem mass spectrometry LC-ESI-MSMS. The antioxidant capacity and antitumor activity of the three extracts were tested on MCF-7 and Hep2. The average total phenolic contents of E. creticum leaves, flowers, and roots were 12.43  0.24, 10.09  0.20, and 4.78  0.10 mg of GAEg of dw, respectively. The average total flavonoids were 4.77  0.10, 6.23  0.12, and 5.62  0.12 mg of QEg of dw, respectively. The extracts showed considerable DPPH scavenger activity with average IC50 values of 1.41  0.06, 2.70  0.06, and 3.03  0.05 µgmL, respectively. The average IC50 values of scavenging capacity of NO radicals were 0.11  0.03, 0.69  0.04, and 0.44  0.01 µgmL, respectively. The average IC50 values in MCF-7 were 1.25  0.50, 3.75  0.48, and 1.88  0.28 mgmL, respectively. In Hep2, the average IC50 values were 1.16  0.58, 3.75  0.79, and 1.31  0.47 µgmL, respectively. Phenolic content varied between the different extracts; leaves showed the highest levels of gallic acid 3.60 µgmL, querciti-3-O-galactoside 0.82 µgmL, cyanin chloride 1.52 µgmL, and rosmarinic acid 4.65 µgmL. Roots showed the lowest quantities of the measured phenolics, except for chlorogenic acid 6.00 µgmL and rosmarinic acid 1.86 µgmL. The three extracts of E. creticum possess in vitro antitumor activities, which could be utilized as an adjunct with chemotherapy.

 Natural products are potential sources of prospective antitumor agents. The phenolic and flavonoid contents of leaves, flowers, and roots of Eryngium creticum were quantified using liquid chromatography-tandem mass spectrometry LC-ESI-MSMS. The antioxidant capacity and antitumor activity of the three extracts were tested on MCF-7 and Hep2. The average total phenolic contents of E. creticum leaves, flowers, and roots were 12.43  0.24, 10.09  0.20, and 4.78  0.10 mg of GAEg of dw, respectively. The average total flavonoids were 4.77  0.10, 6.23  0.12, and 5.62  0.12 mg of QEg of dw, respectively. The extracts showed considerable DPPH scavenger activity with average IC50 values of 1.41  0.06, 2.70  0.06, and 3.03  0.05 µgmL, respectively. The average IC50 values of scavenging capacity of NO radicals were 0.11  0.03, 0.69  0.04, and 0.44  0.01 µgmL, respectively. The average IC50 values in MCF-7 were 1.25  0.50, 3.75  0.48, and 1.88  0.28 mgmL, respectively. In Hep2, the average IC50 values were 1.16  0.58, 3.75  0.79, and 1.31  0.47 µgmL, respectively. Phenolic content varied between the different extracts; leaves showed the highest levels of gallic acid 3.60 µgmL, querciti-3-O-galactoside 0.82 µgmL, cyanin chloride 1.52 µgmL, and rosmarinic acid 4.65 µgmL. Roots showed the lowest quantities of the measured phenolics, except for chlorogenic acid 6.00 µgmL and rosmarinic acid 1.86 µgmL. The three extracts of E. creticum possess in vitro antitumor activities, which could be utilized as an adjunct with chemotherapy.

 Natural products are potential sources of prospective antitumor agents. The phenolic and flavonoid contents of leaves, flowers, and roots of Eryngium creticum were quantified using liquid chromatography-tandem mass spectrometry LC-ESI-MSMS. The antioxidant capacity and antitumor activity of the three extracts were tested on MCF-7 and Hep2. The average total phenolic contents of E. creticum leaves, flowers, and roots were 12.43  0.24, 10.09  0.20, and 4.78  0.10 mg of GAEg of dw, respectively. The average total flavonoids were 4.77  0.10, 6.23  0.12, and 5.62  0.12 mg of QEg of dw, respectively. The extracts showed considerable DPPH scavenger activity with average IC50 values of 1.41  0.06, 2.70  0.06, and 3.03  0.05 µgmL, respectively. The average IC50 values of scavenging capacity of NO radicals were 0.11  0.03, 0.69  0.04, and 0.44  0.01 µgmL, respectively. The average IC50 values in MCF-7 were 1.25  0.50, 3.75  0.48, and 1.88  0.28 mgmL, respectively. In Hep2, the average IC50 values were 1.16  0.58, 3.75  0.79, and 1.31  0.47 µgmL, respectively. Phenolic content varied between the different extracts; leaves showed the highest levels of gallic acid 3.60 µgmL, querciti-3-O-galactoside 0.82 µgmL, cyanin chloride 1.52 µgmL, and rosmarinic acid 4.65 µgmL. Roots showed the lowest quantities of the measured phenolics, except for chlorogenic acid 6.00 µgmL and rosmarinic acid 1.86 µgmL. The three extracts of E. creticum possess in vitro antitumor activities, which could be utilized as an adjunct with chemotherapy.

Article Details

Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Clinical Pharmacy and Pharmacy Practice

Journal:

Journal of Research in Pharmacy Vol.26 Issue 4, pp.770-780

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347269

pISSN:

2630-6344

: Eryngium

plant extract

phenolic compounds

cytotoxicity

breast cancer

antitumor agents

Issues

Article Details

Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity

Authors: N.A.

Publisher: Marmara University

Main Topics: Clinical Pharmacy and Pharmacy Practice

Journal: Journal of Research in Pharmacy Vol.26 Issue 4, pp.770-780

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001347269

pISSN: 2630-6344

: Eryngium

plant extract

phenolic compounds

cytotoxicity

breast cancer

antitumor agents

Issues

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Clinical Pharmacy and Pharmacy Practice

Journal:

Journal of Research in Pharmacy Vol.26 Issue 4, pp.770-780

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347269

pISSN:

2630-6344