Molecular Diagnosis Of Clostridium piliforme, The Causative Agent Of Tyzzer’s Disease In Mice And Rats

Ufuk Ülker; Sibel Kızıl; Efsun Melike Çeçen; Elif Aydın

Article Details

Molecular Diagnosis Of Clostridium piliforme, The Causative Agent Of Tyzzer’s Disease In Mice And Rats

Authors:

Ufuk Ülker | Sibel Kızıl | Efsun Melike Çeçen | Elif Aydın

Publisher:

Ataturk University

Main Topics:

Hayvan Hücresi ve Moleküler Biyoloji

Journal:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 2, pp.78-82

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001326724

pISSN:

2791-8645

Keywords:

Abstract:

 Tyzzers disease, caused by Clostridium piliforme C. piliforme, occurs in many species of mammals mainly in rodents, rabbits and foals is an acute, epizootic bacterial disease that can result in mortality. In our study, C. piliforme was investigated in feces samples from mice and rats in a laboratory experimental animals center by PCR technique. C. piliforme was detected in 44 83.01 of a total of 53 mouse feces of different ages. Of the mice found positive, 28 73.6 were female and 16 88.8 were male. C. piliforme was detected in 40 90.9 of a total of 44 rats feces of different ages. Of the rats found positive, 25 96.1 were female and 15 83.3 were male. C. piliforme, the causative agent of Tyzzers disease, was detected at a high rate in mice and rats examined by the classical PCR method. When evaluated in terms of gender, 28 73.6 of the 40 mice positive for Tyzerrs disease were female and 16 88.8 were male; While 25 96.1 of the rats were female, 15 83.3 were male. As a result, Tyzerrs disease caused by C. piliforme was detected at a high rate in mice and rats by classical PCR. When evaluated by age, the disease was detected to be higher in mice at the ages of 4, 5 and 8, and in rats at the ages of 4, 5, 6 and 7. Due to the reliability of the analyses and the high risk of transmission to other animals in centers where laboratory animals are raised, Tyzzers disease should be checked and necessary precautions should be taken to prevent the disease.

 Tyzzers disease, caused by Clostridium piliforme C. piliforme, occurs in many species of mammals mainly in rodents, rabbits and foals is an acute, epizootic bacterial disease that can result in mortality. In our study, C. piliforme was investigated in feces samples from mice and rats in a laboratory experimental animals center by PCR technique. C. piliforme was detected in 44 83.01 of a total of 53 mouse feces of different ages. Of the mice found positive, 28 73.6 were female and 16 88.8 were male. C. piliforme was detected in 40 90.9 of a total of 44 rats feces of different ages. Of the rats found positive, 25 96.1 were female and 15 83.3 were male. C. piliforme, the causative agent of Tyzzers disease, was detected at a high rate in mice and rats examined by the classical PCR method. When evaluated in terms of gender, 28 73.6 of the 40 mice positive for Tyzerrs disease were female and 16 88.8 were male; While 25 96.1 of the rats were female, 15 83.3 were male. As a result, Tyzerrs disease caused by C. piliforme was detected at a high rate in mice and rats by classical PCR. When evaluated by age, the disease was detected to be higher in mice at the ages of 4, 5 and 8, and in rats at the ages of 4, 5, 6 and 7. Due to the reliability of the analyses and the high risk of transmission to other animals in centers where laboratory animals are raised, Tyzzers disease should be checked and necessary precautions should be taken to prevent the disease.

 Tyzzers disease, caused by Clostridium piliforme C. piliforme, occurs in many species of mammals mainly in rodents, rabbits and foals is an acute, epizootic bacterial disease that can result in mortality. In our study, C. piliforme was investigated in feces samples from mice and rats in a laboratory experimental animals center by PCR technique. C. piliforme was detected in 44 83.01 of a total of 53 mouse feces of different ages. Of the mice found positive, 28 73.6 were female and 16 88.8 were male. C. piliforme was detected in 40 90.9 of a total of 44 rats feces of different ages. Of the rats found positive, 25 96.1 were female and 15 83.3 were male. C. piliforme, the causative agent of Tyzzers disease, was detected at a high rate in mice and rats examined by the classical PCR method. When evaluated in terms of gender, 28 73.6 of the 40 mice positive for Tyzerrs disease were female and 16 88.8 were male; While 25 96.1 of the rats were female, 15 83.3 were male. As a result, Tyzerrs disease caused by C. piliforme was detected at a high rate in mice and rats by classical PCR. When evaluated by age, the disease was detected to be higher in mice at the ages of 4, 5 and 8, and in rats at the ages of 4, 5, 6 and 7. Due to the reliability of the analyses and the high risk of transmission to other animals in centers where laboratory animals are raised, Tyzzers disease should be checked and necessary precautions should be taken to prevent the disease.

 Clostridium piliforme C. piliformenin neden olduğu Tyzzer hastalığı, başta kemirgenler, tavşanlar ve taylar olmak üzere birçok memeli türünde ortaya çıkan, ölümle sonuçlanabilen akut, epizootik bir bakteriyel hastalıktır. Çalışmamızda laboratuvar deney hayvanları merkezinde yetiştirilen fare ve ratlardan alınan dışkı örneklerinde, PCR ile C. piliforme araştırıldı. Farklı yaşlardaki toplam 53 fare dışkısının 44ünde 83,01 C. piliforme tespit edildi. Pozitif bulunan farelerin 28i 73,6 dişi, 16sı 88,8 erkekti. Farklı yaşlardaki toplam 44 ratın dışkısının 40ında 90,9 C. piliforme tespit edildi. Pozitif bulunan sıçanların 25i 96,1 dişi, 15i 83,3 erkekti. Tyzzerz Hastalığının etkeni olan C. piliforme, klasik PCR yöntemiyle incelenen fare ve ratlarda yüksek oranda tespit edildi. Cinsiyet olarak değerlendirildiğinde, Tyzerrs Hastalığı 40 adet pozitif bulunan farelerin 28i 73.6 dişi, 16sı 88.8 erkek; ratların ise 25i 96.1 dişi iken, 15i 83.3 erkek idi. Sonuç olarak C. piliformenin neden olduğu Tyzerrs Hastalığı, klasik PCR ile fare ve ratlarda yüksek oranda tespit edildi. Yaş olarak değerlendirme yapıldığında hastalık farelerde 4, 5 ve 8. yaşlarda, ratlarda ise 4, 5, 6 ve 7. yaşlarda daha yüksek saptandı. Analizlerin güvenilirliği ve laboratuvar hayvanlarının yetiştirildiği merkezlerde, diğer hayvanlara bulaşma riskinin yüksek olması nedeniyle, Tyzzerz Hastalığının kontrol edilmesi ve hastalığın önlenmesi için gerekli önlemlerin alınması gerekmektedir.

Article Details

Molecular Diagnosis Of Clostridium piliforme, The Causative Agent Of Tyzzer’s Disease In Mice And Rats

Authors:

Ufuk Ülker | Sibel Kızıl | Efsun Melike Çeçen | Elif Aydın

Publisher:

Ataturk University

Main Topics:

Hayvan Hücresi ve Moleküler Biyoloji

Journal:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 2, pp.78-82

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001326724

pISSN:

2791-8645

Clostridium piliforme

PCR

Tyzzer’s Disease

ratClostridium piliforme

PCR

rat

Tyzeer's Hastalığı

Issues

Article Details

Authors: Ufuk Ülker | Sibel Kızıl | Efsun Melike Çeçen | Elif Aydın

Publisher: Ataturk University

Main Topics: Hayvan Hücresi ve Moleküler Biyoloji

Journal: Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 2, pp.78-82

Document Type: Article

Publication Date: 9/2024

Document Language: English

Document ID (Org No.): 2001326724

pISSN: 2791-8645

Issues

Authors:

Ufuk Ülker | Sibel Kızıl | Efsun Melike Çeçen | Elif Aydın

Publisher:

Ataturk University

Main Topics:

Hayvan Hücresi ve Moleküler Biyoloji

Journal:

Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi Vol.4 Issue 2, pp.78-82

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001326724

pISSN:

2791-8645