Cytotoxic Assay of Nigella sativa Leaf Callus Extract (Thymol) on Hep-2 Cell Line Using ELISA Assay

Zaynab S. Abdel Gany; Mayasaa F. Mahdi

doi:10.31351/vol17iss2pp63-66

Article Details

Cytotoxic Assay of Nigella sativa Leaf Callus Extract (Thymol) on Hep-2 Cell Line Using ELISA Assay

Authors:

Zaynab S. Abdel Gany | Mayasaa F. Mahdi

Publisher:

University of Baghdad

Journal:

Iraqi Journal of Pharmaceutical Sciences Vol.17 Issue 2, pp.63-66

Document Type:

Article

Publication Date:

1/2008

Document Language:

English

Document ID (Org No.):

2001362576

pISSN:

2521-3512

DOI:

10.31351/vol17iss2pp63-66

Abstract:

 Extract from cell culture of medicinal plant like Nigella sativa have been assessed for its cytotoxic properties. Thymol is likely responsible for the theraputic effects of  Nigella sativa leaf callus extract. In this short study the inhibitory effect of Nigella sativa leaf callus extract (Thymol) has been studied on Human Lorgnx Epidrmoid Carcinoma (Hep-2) cell line during different exposure period of time (24, 48 and 72 hrs.) using different concentration of the extract (1000, 500, 400, 300, 200 and 100 Âµg/ml). The optical density of the Hep-2 cells has been readed on 492 nm wave length. Thymol â€“induced cytotoxicity was (500 Âµg/ml) which inhibit cell growing compared to the control and this ratio increased at the 48 hrs of exopsure and stopped at 72 hrs.
Key wards: Nigella sativa, callus extract, cell line, ELISA assay.

Article Details

Cytotoxic Assay of Nigella sativa Leaf Callus Extract (Thymol) on Hep-2 Cell Line Using ELISA Assay

Authors: Zaynab S. Abdel Gany | Mayasaa F. Mahdi

Publisher: University of Baghdad

Journal: Iraqi Journal of Pharmaceutical Sciences Vol.17 Issue 2, pp.63-66

Document Type: Article

Publication Date: 1/2008

Document Language: English

Document ID (Org No.): 2001362576

pISSN: 2521-3512

DOI: 10.31351/vol17iss2pp63-66

Issues

Authors:

Zaynab S. Abdel Gany | Mayasaa F. Mahdi

Publisher:

University of Baghdad

Journal:

Iraqi Journal of Pharmaceutical Sciences Vol.17 Issue 2, pp.63-66

Document Type:

Article

Publication Date:

1/2008

Document Language:

English

Document ID (Org No.):

2001362576

pISSN:

2521-3512

DOI:

10.31351/vol17iss2pp63-66