A potential role of extracellular DNA in biofilm and ciprofloxacin resistance

Hind Tahseen Ibrahim; Ali A. Mussa; Harith Jabbar Fahad Al-Mathkhury

doi:10.22317/jcms.v9i2.1338

Article Details

A potential role of extracellular DNA in biofilm and ciprofloxacin resistance

Authors:

Hind Tahseen Ibrahim | Ali A. Mussa | Harith Jabbar Fahad Al-Mathkhury

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.9 Issue 2, pp.0-0

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001379547

pISSN:

2413-0516

DOI:

10.22317/jcms.v9i2.1338

Keywords:

Abstract:

 Objectives: This study aims to broaden our knowledge of the role of eDNA in bacterial biofilms and antibiotic-resistance gene transfer among isolates.
Methods: Staphylococcus aureus, E. coli, and Pseudomonas aeruginosa were isolated from different non-repeated 170 specimens. The bacterial isolates were identified using morphological and molecular methods. Different concentrations of genomic DNA were tested for their potential role in biofilms formed by study isolates employing microtiter plate assay. Ciprofloxacin resistance was identified by detecting a mutation in gyrA and parC.
Results: The biofilm intensity significantly decreased (P < 0.05) concerning S. aureus isolates and insignificantly (P > 0.05) concerning E. coli isolates. Yet, one E. coli isolate's biofilm was significantly decreased (P < 0.05) linearly with increasing eDNA. Of considerable interest, the addition of eDNA led to a significant increase (P < 0.05) in the biofilm of the two-tested P. aeruginosa isolates. Moreover, eDNA participated in transferring Ciprofloxacin resistance to the sensitive isolate when it presents in its biofilm.
Conclusion: eDNA has a dual effect on bacterial biofilms either supportive or suppressive following bacterial species per se. Also, it seems to play an important role in antibiotic resistance within the biofilm.

Article Details

A potential role of extracellular DNA in biofilm and ciprofloxacin resistance

Authors:

Hind Tahseen Ibrahim | Ali A. Mussa | Harith Jabbar Fahad Al-Mathkhury

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.9 Issue 2, pp.0-0

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001379547

pISSN:

2413-0516

DOI:

10.22317/jcms.v9i2.1338

eDNA

Biofilm

Staphylococcus aureus

Escherichia coli

Pseudomonas aeruginosa

Issues

Article Details

A potential role of extracellular DNA in biofilm and ciprofloxacin resistance

Authors: Hind Tahseen Ibrahim | Ali A. Mussa | Harith Jabbar Fahad Al-Mathkhury

Publisher: Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal: Journal of Contemporary Medical Sciences Vol.9 Issue 2, pp.0-0

Document Type: Article

Publication Date: 4/2023

Document Language: English

Document ID (Org No.): 2001379547

pISSN: 2413-0516

DOI: 10.22317/jcms.v9i2.1338

eDNA

Biofilm

Staphylococcus aureus

Escherichia coli

Pseudomonas aeruginosa

Issues

Authors:

Hind Tahseen Ibrahim | Ali A. Mussa | Harith Jabbar Fahad Al-Mathkhury

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.9 Issue 2, pp.0-0

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001379547

pISSN:

2413-0516

DOI:

10.22317/jcms.v9i2.1338