Comparison of MALDI TOF MS profiling and 16S rRNA gene identification of presumptive lactic acid bacteria isolated from the traditional Algerian date product Btana

Abekhti Abdelkader; Cnockaert Margo; Aerts Maarten; Daube Georges; Vandamme Peter; Tahri Assia; Taminiau Bernard

Article Details

Comparison of MALDI TOF MS profiling and 16S rRNA gene identification of presumptive lactic acid bacteria isolated from the traditional Algerian date product Btana

Authors:

Abekhti Abdelkader | Cnockaert Margo | Aerts Maarten | Daube Georges | Vandamme Peter | Tahri Assia | Taminiau Bernard

Publisher:

Université de Béchar

Journal:

Applied Biology in Saharan Areas Vol.3 Issue 3, pp.27-43

Document Type:

Article

Publication Date:

6/2021

Document Language:

English

Document ID (Org No.):

2001541647

pISSN:

2716-9480

Keywords:

Abstract:

 The present study sat the purpose to compare the accuracy of identification of 68 strains lactic  acid bacteria originating from Btana samples (Bouchegroun, Sali, Algeria)  Btana by means of 16s RNA sequencing and Matrix-assisted laser desorption ⁄ ioni-zation time-of-ﬂight mass spectrometry (MALDI-TOFMS) MALDI TOF. Dilution of Btana samples were cultured on MRS, M17 and MSE  media. For MALDI TOF identification, a cell extract was prepared from each isolates then coated with matrix of 1 μL of a 0.5% (w/v) alpha-cyano-4-hydroxycinnamic acid solution (α-CHCA). The best quality spectra was selected then compared against an in-house (LMG)  built reference database. Five assigned cluster identified as Weissella paramesenteroides, Weissella cibaria/confuse, Leuconostoc citreum, Leuconostoc pseudomesenteroides, and Lactobacillus plantarum and one AAB species Gluconobacter cerinus. A representative strain of each cluster was selected for identification by 16S rRNA gene analysis. Sequences comparison with Genbank database confirmed accurately the strain’s identity made already by MALDI-TOF. According to API 50 CHL essay, 7 carbohydrates (D-glucose, D-fructose, D-mannose, N-acetyl glucosamine, D-maltose, D-lactose, D-saccharose were systematically used by the five  LAB strains. Which support that the identified species are adapted to the high sugar environment of Btana.

Article Details

Comparison of MALDI TOF MS profiling and 16S rRNA gene identification of presumptive lactic acid bacteria isolated from the traditional Algerian date product Btana

Authors:

Abekhti Abdelkader | Cnockaert Margo | Aerts Maarten | Daube Georges | Vandamme Peter | Tahri Assia | Taminiau Bernard

Publisher:

Université de Béchar

Journal:

Applied Biology in Saharan Areas Vol.3 Issue 3, pp.27-43

Document Type:

Article

Publication Date:

6/2021

Document Language:

English

Document ID (Org No.):

2001541647

pISSN:

2716-9480

Btana

date fruit

traditional food

lactic acid bacteria

high sugar food

MALDI-TOF MS

Issues

Article Details

Comparison of MALDI TOF MS profiling and 16S rRNA gene identification of presumptive lactic acid bacteria isolated from the traditional Algerian date product Btana

Authors: Abekhti Abdelkader | Cnockaert Margo | Aerts Maarten | Daube Georges | Vandamme Peter | Tahri Assia | Taminiau Bernard

Publisher: Université de Béchar

Journal: Applied Biology in Saharan Areas Vol.3 Issue 3, pp.27-43

Document Type: Article

Publication Date: 6/2021

Document Language: English

Document ID (Org No.): 2001541647

pISSN: 2716-9480

Btana

date fruit

traditional food

lactic acid bacteria

high sugar food

MALDI-TOF MS

Issues

Authors:

Abekhti Abdelkader | Cnockaert Margo | Aerts Maarten | Daube Georges | Vandamme Peter | Tahri Assia | Taminiau Bernard

Publisher:

Université de Béchar

Journal:

Applied Biology in Saharan Areas Vol.3 Issue 3, pp.27-43

Document Type:

Article

Publication Date:

6/2021

Document Language:

English

Document ID (Org No.):

2001541647

pISSN:

2716-9480