The Effects of Refrigerated Storage Time on Sialic Acid and Nitric Oxide Levels and Oxidant Antioxidant System of Human Milk

Begüm Gürel Gökmen; Tuğba Tunalı-akbay

Article Details

The Effects of Refrigerated Storage Time on Sialic Acid and Nitric Oxide Levels and Oxidant Antioxidant System of Human Milk

Authors:

Begüm Gürel Gökmen | Tuğba Tunalı-akbay

Publisher:

Istanbul University

Main Topics:

Plant Cell and Molecular Biology | Animal Cell and Molecular Biology

Journal:

European Journal of Biology Vol.82 Issue 2, pp.243-250

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001310873

pISSN:

2602-2575

Keywords:

Abstract:

 Objective: Human milk HM is a marvelous nutrition that serves all the needs of infants in the first six months with the vitamins, minerals, proteins, carbohydrates, and lipids it contains. For the first 4-6 months of a babys life, Nursing is accepted as the most beneficial and recommended feeding method. The greatest technique for providing nutrition in the absence of breastfeeding is through expressed HM. In this case, milk storage conditions become critical. The proper storage of HM is essential for preserving the nutritional and antioxidant properties of HM. This study aims to examine the effects of storing HM in the refrigerator. Materials and Methods: The effects of storing HM in the refrigerator were examined for four days with regard to the protein profile oxidant-antioxidant balance and nitric oxide NO and sialic acid SA levels. Results: Total protein TP levels decreased gradually over the four days. In the SDS-PAGE electrophoresis method, the heavy chain sIgA and -casein bands also disappeared in HM. While glutathione-S-transferase and superoxide dismutase activities decreased significantly during the first two days, their activities fell below the detection limit in the last two days. While the glutathione level and catalase activity also decreased gradually over the four days, the malondialdehyde, SA, and NO levels increased significantly. Conclusion: HM can be safely stored in the refrigerator for two days due to the TP, SA, and NO levels, as well as the antioxidant enzyme activities, remaining unchanged from the first day of expressing HM.

 Objective: Human milk HM is a marvelous nutrition that serves all the needs of infants in the first six months with the vitamins, minerals, proteins, carbohydrates, and lipids it contains. For the first 4-6 months of a babys life, Nursing is accepted as the most beneficial and recommended feeding method. The greatest technique for providing nutrition in the absence of breastfeeding is through expressed HM. In this case, milk storage conditions become critical. The proper storage of HM is essential for preserving the nutritional and antioxidant properties of HM. This study aims to examine the effects of storing HM in the refrigerator. Materials and Methods: The effects of storing HM in the refrigerator were examined for four days with regard to the protein profile oxidant-antioxidant balance and nitric oxide NO and sialic acid SA levels. Results: Total protein TP levels decreased gradually over the four days. In the SDS-PAGE electrophoresis method, the heavy chain sIgA and -casein bands also disappeared in HM. While glutathione-S-transferase and superoxide dismutase activities decreased significantly during the first two days, their activities fell below the detection limit in the last two days. While the glutathione level and catalase activity also decreased gradually over the four days, the malondialdehyde, SA, and NO levels increased significantly. Conclusion: HM can be safely stored in the refrigerator for two days due to the TP, SA, and NO levels, as well as the antioxidant enzyme activities, remaining unchanged from the first day of expressing HM.

 Objective: Human milk HM is a marvelous nutrition that serves all the needs of infants in the first six months with the vitamins, minerals, proteins, carbohydrates, and lipids it contains. For the first 4-6 months of a babys life, Nursing is accepted as the most beneficial and recommended feeding method. The greatest technique for providing nutrition in the absence of breastfeeding is through expressed HM. In this case, milk storage conditions become critical. The proper storage of HM is essential for preserving the nutritional and antioxidant properties of HM. This study aims to examine the effects of storing HM in the refrigerator. Materials and Methods: The effects of storing HM in the refrigerator were examined for four days with regard to the protein profile oxidant-antioxidant balance and nitric oxide NO and sialic acid SA levels. Results: Total protein TP levels decreased gradually over the four days. In the SDS-PAGE electrophoresis method, the heavy chain sIgA and -casein bands also disappeared in HM. While glutathione-S-transferase and superoxide dismutase activities decreased significantly during the first two days, their activities fell below the detection limit in the last two days. While the glutathione level and catalase activity also decreased gradually over the four days, the malondialdehyde, SA, and NO levels increased significantly. Conclusion: HM can be safely stored in the refrigerator for two days due to the TP, SA, and NO levels, as well as the antioxidant enzyme activities, remaining unchanged from the first day of expressing HM.

 Objective: Human milk HM is a marvelous nutrition that serves all the needs of infants in the first six months with the vitamins, minerals, proteins, carbohydrates, and lipids it contains. For the first 4-6 months of a babys life, Nursing is accepted as the most beneficial and recommended feeding method. The greatest technique for providing nutrition in the absence of breastfeeding is through expressed HM. In this case, milk storage conditions become critical. The proper storage of HM is essential for preserving the nutritional and antioxidant properties of HM. This study aims to examine the effects of storing HM in the refrigerator. Materials and Methods: The effects of storing HM in the refrigerator were examined for four days with regard to the protein profile oxidant-antioxidant balance and nitric oxide NO and sialic acid SA levels. Results: Total protein TP levels decreased gradually over the four days. In the SDS-PAGE electrophoresis method, the heavy chain sIgA and -casein bands also disappeared in HM. While glutathione-S-transferase and superoxide dismutase activities decreased significantly during the first two days, their activities fell below the detection limit in the last two days. While the glutathione level and catalase activity also decreased gradually over the four days, the malondialdehyde, SA, and NO levels increased significantly. Conclusion: HM can be safely stored in the refrigerator for two days due to the TP, SA, and NO levels, as well as the antioxidant enzyme activities, remaining unchanged from the first day of expressing HM.

Article Details

The Effects of Refrigerated Storage Time on Sialic Acid and Nitric Oxide Levels and Oxidant Antioxidant System of Human Milk

Authors:

Begüm Gürel Gökmen | Tuğba Tunalı-akbay

Publisher:

Istanbul University

Main Topics:

Plant Cell and Molecular Biology | Animal Cell and Molecular Biology

Journal:

European Journal of Biology Vol.82 Issue 2, pp.243-250

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001310873

pISSN:

2602-2575

Human milk

storage

oxidant-antioxidant system

nitric oxide

sialic acid

SDS-PAGE electrophoresis

Issues

Article Details

The Effects of Refrigerated Storage Time on Sialic Acid and Nitric Oxide Levels and Oxidant Antioxidant System of Human Milk

Authors: Begüm Gürel Gökmen | Tuğba Tunalı-akbay

Publisher: Istanbul University

Main Topics: Plant Cell and Molecular Biology | Animal Cell and Molecular Biology

Journal: European Journal of Biology Vol.82 Issue 2, pp.243-250

Document Type: Article

Publication Date: 12/2023

Document Language: English

Document ID (Org No.): 2001310873

pISSN: 2602-2575

Human milk

storage

oxidant-antioxidant system

nitric oxide

sialic acid

SDS-PAGE electrophoresis

Issues

Authors:

Begüm Gürel Gökmen | Tuğba Tunalı-akbay

Publisher:

Istanbul University

Main Topics:

Plant Cell and Molecular Biology | Animal Cell and Molecular Biology

Journal:

European Journal of Biology Vol.82 Issue 2, pp.243-250

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001310873

pISSN:

2602-2575