Effect of Ultrasound-Assisted Maceration on Yield Percentage, Chemical Compound and Antimicrobial Activity of Citrus nobilis Peel Essential Oil

Eko Widodo

Article Details

Effect of Ultrasound-Assisted Maceration on Yield Percentage, Chemical Compound and Antimicrobial Activity of Citrus nobilis Peel Essential Oil

Authors:

Eko Widodo

Publisher:

Van Yuzuncu Yıl University

Main Topics:

Animal Nutrition

Journal:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 4, pp.656-668

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001333261

pISSN:

1308-7576

Keywords:

Abstract:

 Increasing concerns about the activity of antibacterial growth promoters have prompted the need to discover alternatives from natural materials that can be found in various types of plants and herbs. Siamese orange peels Citrus nobilis are one of the plentiful organic waste-containing essential oils known for their antibacterial, antioxidant, and other biological properties promising as the potential alternative to antibiotic growth promoters in livestock. This study aimed to evaluate the antimicrobial properties of orange peel essential oil between 2 different extraction methods and the effect of different solvent ratios on the yield of citrus essential oil, chemical compounds, and the antibacterial activity against Escherichia coli and Lactobacillus casei. The evaluated methods were ultrasonic-macerated citrus essential oil UMCEO and conventional-macerated citrus essential oil CMCEO, while the solvent ratios were 1:2, 1:4, and 1:6. The result showed that the solvent ratio was found to significantly give effect P0.05 to the yield of citrus peel essential oil with a solvent ratio of 1:2 obtaining the highest yield. Pretreating the citrus peel with ultrasound before hydro-distillation did not give a significant effect P0.05 on the chemical compounds of the citrus essential oil as analyzed by GC-MS. Both citrus essential oils were predominantly composed of cycloheptane CMCEO: 48.5; UMCEO: 44.09 and d-limonene CMCEO: 26.76; UMCEO: 36.02. The CMCEO showed a higher inhibition zone against Escherichia coli and Lactobacillus casei. The conclusion is that CMCEO seems to produce citrus peel essential oil with a stronger antibacterial effect against Escherichia coli and Lactobacillus casei, in particular with solvent ratio 1:2.

 Increasing concerns about the activity of antibacterial growth promoters have prompted the need to discover alternatives from natural materials that can be found in various types of plants and herbs. Siamese orange peels Citrus nobilis are one of the plentiful organic waste-containing essential oils known for their antibacterial, antioxidant, and other biological properties promising as the potential alternative to antibiotic growth promoters in livestock. This study aimed to evaluate the antimicrobial properties of orange peel essential oil between 2 different extraction methods and the effect of different solvent ratios on the yield of citrus essential oil, chemical compounds, and the antibacterial activity against Escherichia coli and Lactobacillus casei. The evaluated methods were ultrasonic-macerated citrus essential oil UMCEO and conventional-macerated citrus essential oil CMCEO, while the solvent ratios were 1:2, 1:4, and 1:6. The result showed that the solvent ratio was found to significantly give effect P0.05 to the yield of citrus peel essential oil with a solvent ratio of 1:2 obtaining the highest yield. Pretreating the citrus peel with ultrasound before hydro-distillation did not give a significant effect P0.05 on the chemical compounds of the citrus essential oil as analyzed by GC-MS. Both citrus essential oils were predominantly composed of cycloheptane CMCEO: 48.5; UMCEO: 44.09 and d-limonene CMCEO: 26.76; UMCEO: 36.02. The CMCEO showed a higher inhibition zone against Escherichia coli and Lactobacillus casei. The conclusion is that CMCEO seems to produce citrus peel essential oil with a stronger antibacterial effect against Escherichia coli and Lactobacillus casei, in particular with solvent ratio 1:2.

 Increasing concerns about the activity of antibacterial growth promoters have prompted the need to discover alternatives from natural materials that can be found in various types of plants and herbs. Siamese orange peels Citrus nobilis are one of the plentiful organic waste-containing essential oils known for their antibacterial, antioxidant, and other biological properties promising as the potential alternative to antibiotic growth promoters in livestock. This study aimed to evaluate the antimicrobial properties of orange peel essential oil between 2 different extraction methods and the effect of different solvent ratios on the yield of citrus essential oil, chemical compounds, and the antibacterial activity against Escherichia coli and Lactobacillus casei. The evaluated methods were ultrasonic-macerated citrus essential oil UMCEO and conventional-macerated citrus essential oil CMCEO, while the solvent ratios were 1:2, 1:4, and 1:6. The result showed that the solvent ratio was found to significantly give effect P0.05 to the yield of citrus peel essential oil with a solvent ratio of 1:2 obtaining the highest yield. Pretreating the citrus peel with ultrasound before hydro-distillation did not give a significant effect P0.05 on the chemical compounds of the citrus essential oil as analyzed by GC-MS. Both citrus essential oils were predominantly composed of cycloheptane CMCEO: 48.5; UMCEO: 44.09 and d-limonene CMCEO: 26.76; UMCEO: 36.02. The CMCEO showed a higher inhibition zone against Escherichia coli and Lactobacillus casei. The conclusion is that CMCEO seems to produce citrus peel essential oil with a stronger antibacterial effect against Escherichia coli and Lactobacillus casei, in particular with solvent ratio 1:2.

 Increasing concerns about the activity of antibacterial growth promoters have prompted the need to discover alternatives from natural materials that can be found in various types of plants and herbs. Siamese orange peels Citrus nobilis are one of the plentiful organic waste-containing essential oils known for their antibacterial, antioxidant, and other biological properties promising as the potential alternative to antibiotic growth promoters in livestock. This study aimed to evaluate the antimicrobial properties of orange peel essential oil between 2 different extraction methods and the effect of different solvent ratios on the yield of citrus essential oil, chemical compounds, and the antibacterial activity against Escherichia coli and Lactobacillus casei. The evaluated methods were ultrasonic-macerated citrus essential oil UMCEO and conventional-macerated citrus essential oil CMCEO, while the solvent ratios were 1:2, 1:4, and 1:6. The result showed that the solvent ratio was found to significantly give effect P0.05 to the yield of citrus peel essential oil with a solvent ratio of 1:2 obtaining the highest yield. Pretreating the citrus peel with ultrasound before hydro-distillation did not give a significant effect P0.05 on the chemical compounds of the citrus essential oil as analyzed by GC-MS. Both citrus essential oils were predominantly composed of cycloheptane CMCEO: 48.5; UMCEO: 44.09 and d-limonene CMCEO: 26.76; UMCEO: 36.02. The CMCEO showed a higher inhibition zone against Escherichia coli and Lactobacillus casei. The conclusion is that CMCEO seems to produce citrus peel essential oil with a stronger antibacterial effect against Escherichia coli and Lactobacillus casei, in particular with solvent ratio 1:2.

Article Details

Effect of Ultrasound-Assisted Maceration on Yield Percentage, Chemical Compound and Antimicrobial Activity of Citrus nobilis Peel Essential Oil

Authors:

Eko Widodo

Publisher:

Van Yuzuncu Yıl University

Main Topics:

Animal Nutrition

Journal:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 4, pp.656-668

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001333261

pISSN:

1308-7576

extraction

citrus peel

essential oil

anctibacterial

yield

Issues

Article Details

Effect of Ultrasound-Assisted Maceration on Yield Percentage, Chemical Compound and Antimicrobial Activity of Citrus nobilis Peel Essential Oil

Authors: Eko Widodo

Publisher: Van Yuzuncu Yıl University

Main Topics: Animal Nutrition

Journal: Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 4, pp.656-668

Document Type: Article

Publication Date: 12/2024

Document Language: English

Document ID (Org No.): 2001333261

pISSN: 1308-7576

extraction

citrus peel

essential oil

anctibacterial

yield

Issues

Authors:

Eko Widodo

Publisher:

Van Yuzuncu Yıl University

Main Topics:

Animal Nutrition

Journal:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.34 Issue 4, pp.656-668

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001333261

pISSN:

1308-7576