Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis

Zeliha DEMİREL; Esra IMAMOGLU; İrem DENİZ; Meltem CONK DALAY

doi:https://doi.org/10.18466/cbayarfbe.426444

Article Details

Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis

Authors:

Zeliha DEMİREL | Esra IMAMOGLU | İrem DENİZ | Meltem CONK DALAY

Publisher:

Celal Bayar University

Main Topics:

Applied physics

Journal:

Celal Bayar University Journal of Science 2018 Vol.14 Issue 4, pp.405-412

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000706166

Frequency:

Quarterly

eISSN:

1305-1385

pISSN:

1305-130X

DOI:

https://doi.org/10.18466/cbayarfbe.426444

Abstract:

 


The preservation of microalgae
in a stable state is a fundamental requirement in pharmaceutical, agricultural,
environmental sciences and different industries. Cryopreservation is widely
stabilized for achieving long-term storage and has been applied to an
increasingly diverse range of microalgae and cell cultures. The continuous storage
of actively growing microalgae strains by routine serial subculture is
relatively time-consuming and this technique has possible contamination risks.
In this study, the optimization of cryopreservation process was carried out for
two different Chlorella strains
using response surface methodology (RSM) with three factors (cryoprotectant
concentration, incubation time and cryopreservation time) including 19 runs. The
optimal cell viability of C. zofingiensis
was found at the dimethyl sulfoxide (DMSO) concentration of 12.89% at the incubation
time of 8.14 min and with the cryopreservation time of 93.45 day, while C.
saccharophila was found at the
DMSO concentration of 12.86 % at the incubation time of 7.99 min and at
cryopreservation time of 95.17 day.

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Article Details

Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis

Authors: Zeliha DEMİREL | Esra IMAMOGLU | İrem DENİZ | Meltem CONK DALAY

Publisher: Celal Bayar University

Main Topics: Applied physics

Journal: Celal Bayar University Journal of Science 2018 Vol.14 Issue 4, pp.405-412

Document Type: Article

Publication Date: 12/2018

Document Language: English

Document ID (Org No.): 2000706166

Frequency: Quarterly

eISSN: 1305-1385

pISSN: 1305-130X

DOI: https://doi.org/10.18466/cbayarfbe.426444

Issues

Authors:

Zeliha DEMİREL | Esra IMAMOGLU | İrem DENİZ | Meltem CONK DALAY

Publisher:

Celal Bayar University

Main Topics:

Applied physics

Journal:

Celal Bayar University Journal of Science 2018 Vol.14 Issue 4, pp.405-412

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000706166

Frequency:

Quarterly

eISSN:

1305-1385

pISSN:

1305-130X

DOI:

https://doi.org/10.18466/cbayarfbe.426444