MicroRNA-16, Bcl-2, and MAPK : Potential Biomarkers for Oral Squamous Cell Carcinoma

El Feky , Noha Mohamed; Amar , Sabreen Gamal Khalil; Sharf El Deen , Abd El Rahman M.; Abd El-Aziz , Enas Alaa El Din

Article Details

MicroRNA-16, Bcl-2, and MAPK : Potential Biomarkers for Oral Squamous Cell Carcinoma

Authors:

El Feky , Noha Mohamed | Amar , Sabreen Gamal Khalil | Sharf El Deen , Abd El Rahman M. | Abd El-Aziz , Enas Alaa El Din

Publisher:

Ain Shams University : Faculty of Dentistry

Main Topics:

Medicine

Journal:

Ain Shams Dental Journal 2025 Vol.37 Issue 1, pp.183-194

Document Type:

Article

Publication Date:

3/2025

Document Language:

English

Document ID (Org No.):

264572

Frequency:

Quarterly

pISSN:

11107642

Descriptors:

Keywords:

Abstract:

 Aim: The objectives of the current study were to assess the level of miRNA‐16 expression in oral squamous cell carcinoma and its effect on cell apoptosis and proliferation following its suppression. Additionally, the study aimed to investigate the mechanisms that underlie the involvement of miRNA-16 and its target genes, Bcl-2 and, MAPK in the progression of OSCC. Materials and methods: The Oral Cavity Squamous Cell Carcinoma cell line (OECM-1) and Normal Human Tongue Fibroblasts (HOrF) were used in this in vitro study. The MTT assay was used to evaluate the cytotoxic effect following the cells' transfection with a miRNA-16 inhibitor. In both treated and untreated cells, the expression levels of the miRNA-16, Bcl-2, and MAPK genes were measured using SYBER green-based quantitative PCR. Tukey's multiple comparisons test and One-way analysis of variance (ANOVA) were used to analyze the data. Results: OECM-1 cells transfected with miRNA-16 inhibitor exhibited a significant increase in proliferation in comparison with the untreated and normal cells. Additionally, a significant increase in the expression of Bcl-2 and MAPK was associated with miRNA-16 inhibition compared to the untreated OECM-1 cells. Conclusion: The findings suggest that miRNA‐16 is a tumor suppressor miRNA that acts by cell proliferation inhibition and apoptosis induction in OSCC by directly targeting Bcl-2 and MAPK. This implies that miRNA‐16 could be a potential therapeutic target for OSCC.

Article Details

MicroRNA-16, Bcl-2, and MAPK : Potential Biomarkers for Oral Squamous Cell Carcinoma

Authors:

El Feky , Noha Mohamed | Amar , Sabreen Gamal Khalil | Sharf El Deen , Abd El Rahman M. | Abd El-Aziz , Enas Alaa El Din

Publisher:

Ain Shams University : Faculty of Dentistry

Main Topics:

Medicine

Journal:

Ain Shams Dental Journal 2025 Vol.37 Issue 1, pp.183-194

Document Type:

Article

Publication Date:

3/2025

Document Language:

English

Document ID (Org No.):

264572

Frequency:

Quarterly

pISSN:

11107642

Descriptors:

MOUTH DISEASES

SQUAMOUS CARCINOMA

DIAGNOSIS

DENTISTRY

OSCC

MiRNA‐16

Bcl-2

MAPK

Issues

Article Details

Authors: El Feky , Noha Mohamed | Amar , Sabreen Gamal Khalil | Sharf El Deen , Abd El Rahman M. | Abd El-Aziz , Enas Alaa El Din

Publisher: Ain Shams University : Faculty of Dentistry

Main Topics: Medicine

Journal: Ain Shams Dental Journal 2025 Vol.37 Issue 1, pp.183-194

Document Type: Article

Publication Date: 3/2025

Document Language: English

Document ID (Org No.): 264572

Frequency: Quarterly

pISSN: 11107642

Descriptors:

Issues

Authors:

El Feky , Noha Mohamed | Amar , Sabreen Gamal Khalil | Sharf El Deen , Abd El Rahman M. | Abd El-Aziz , Enas Alaa El Din

Publisher:

Ain Shams University : Faculty of Dentistry

Main Topics:

Medicine

Journal:

Ain Shams Dental Journal 2025 Vol.37 Issue 1, pp.183-194

Document Type:

Article

Publication Date:

3/2025

Document Language:

English

Document ID (Org No.):

264572

Frequency:

Quarterly

pISSN:

11107642