Butyrate-Mediated Genomic Changes Involved in Non-Specific Host Defenses, Matrix Remodeling and the Immune Response in the Rumen Epithelium of Cows Afflicted with Subacute Ruminal Acidosis

Louis Dionissopoulos; Anne Hermen Laarman; Ousama AlZahal; Sabrina Louise Greenwood; Michael Alexander Steele; Jan Cees Plaizier; James Clyde Matthews; Brian William McBride

doi:10.3844/ajavsp.2013.8.27

Article Details

Butyrate-Mediated Genomic Changes Involved in Non-Specific Host Defenses, Matrix Remodeling and the Immune Response in the Rumen Epithelium of Cows Afflicted with Subacute Ruminal Acidosis

Authors:

Louis Dionissopoulos | Anne Hermen Laarman | Ousama AlZahal | Sabrina Louise Greenwood | Michael Alexander Steele | Jan Cees Plaizier | James Clyde Matthews | Brian William McBride

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.8 Issue 1, pp.8-27

Document Type:

Article

Publication Date:

10/2013

Document Language:

English

Document ID (Org No.):

2001396369

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2013.8.27

Keywords:

Abstract:

 Subacute Ruminal Acidosis (SARA) is a disorder in cattle which can lead to chronic inflammation in the rumen epithelium, known as rumenitis. Butyrate has been shown to attenuate some of the detrimental effects of inflammatory gastroenteral disorders but the molecular mechanisms mediated by butyrate have not been defined. The objective of this study was to define the inflammatory-related genomic changes responsible for the beneficial effects of butyrate. Experimentally, 16 fistulated dairy cows at mid-lactation were fed a SARA-inducing (45% non-fiber carbohydrate) diet beginning 2 days before the beginning of treatment and continuing throughout the experiment. Cows were then evenly divided into treatment groups where a carrier with (n = 8) or without (n = 8) supplemental butyrate (2.5% initial DM intake) was deposited into the rumen daily for 7 days. The minimum rumen pH was higher in cows with supplemental butyrate (4.96±0.09 to 5.20±0.05, p = 0.040), but mean pH, maximum pH and the duration for which rumen pH was below 5.6 was unaffected. Free plasma Lipopolysaccharide (LPS) concentration was unaffected by treatment as was the concentration of Serum Amyloid A (SAA), although the LPS Binding Protein (LBP) concentration was increased by the addition of butyrate to the rumen (6.91±0.29 to 7.93±0.29 µg mL-1, p = 0.024). Of the rumen Short Chain Fatty Acids (SCFA) tested, only butyrate showed a pronounced treatment effect, rising from 8.60±0.94 to 21.60±0.94 mM (p≤0.0001). Plasma Beta-Hydroxybutyrate (BHBA) concentration also increased (799.50±265.24 to 3261.63±265.24 µM, p≤0.001). Butyrate infusion did not affect milk parameters (total fat, lactose, total protein and LOS); however, when related to dry matter intake, milk production efficiency was increased (p = 0.035). Microarray and qRT-PCR analyses of rumen papillae biopsies collected on day 7 found that butyrate administration affected (p≤0.05) the expression of genes involved in Non-Specific Host Defense (NSHD), Remodeling or adaptation (RM) and Immune Response (IR). Of the 49 genes tested by qRT-PCR, 9 (LCN2, MMP1, MUC16, GPX2, CSTA, FUT1, SERPINE2, BCAM, RAC3) were upregulated, 20 (MTOR, AKIRIN2, NFKBIZ, NFKB2, ACVR2A, LAMB1, FRS2, PPARD, LBP, NEDD4L, SGK1, DEDD2, MAP3K8, PARD6B, PLIN2, ADA, HPGD, FMO5, BMP6, TCHH) were downregulated and 20 were unchanged due to butyrate administration in the proximal gastrointestinal tract. These results demonstrate the potential protective effect and molecular mechanisms involved in a novel butyrate treatment for inflammatory gastrointestinal conditions.

Article Details

Butyrate-Mediated Genomic Changes Involved in Non-Specific Host Defenses, Matrix Remodeling and the Immune Response in the Rumen Epithelium of Cows Afflicted with Subacute Ruminal Acidosis

Authors:

Louis Dionissopoulos | Anne Hermen Laarman | Ousama AlZahal | Sabrina Louise Greenwood | Michael Alexander Steele | Jan Cees Plaizier | James Clyde Matthews | Brian William McBride

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.8 Issue 1, pp.8-27

Document Type:

Article

Publication Date:

10/2013

Document Language:

English

Document ID (Org No.):

2001396369

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2013.8.27

Butyrate

Epithelium

SARA

Inflammation

Rumen

Wound Healing

Gene Expression

Issues

Article Details

Authors: Louis Dionissopoulos | Anne Hermen Laarman | Ousama AlZahal | Sabrina Louise Greenwood | Michael Alexander Steele | Jan Cees Plaizier | James Clyde Matthews | Brian William McBride

Publisher: Science Publications

Journal: American Journal of Animal and Veterinary Sciences Vol.8 Issue 1, pp.8-27

Document Type: Article

Publication Date: 10/2013

Document Language: English

Document ID (Org No.): 2001396369

pISSN: 1557-4563

DOI: 10.3844/ajavsp.2013.8.27

Issues

Authors:

Louis Dionissopoulos | Anne Hermen Laarman | Ousama AlZahal | Sabrina Louise Greenwood | Michael Alexander Steele | Jan Cees Plaizier | James Clyde Matthews | Brian William McBride

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.8 Issue 1, pp.8-27

Document Type:

Article

Publication Date:

10/2013

Document Language:

English

Document ID (Org No.):

2001396369

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2013.8.27