Development of Nanoemulsion Formulation Containing Ylang Ylang Essential Oil for Topical Applications, Evaluation of In Vitro Cytotoxicity and ADMET Profile

Nesrin Karabatak; Bahar Gök; Yasemin Budama-kılınc

Article Details

Development of Nanoemulsion Formulation Containing Ylang Ylang Essential Oil for Topical Applications, Evaluation of In Vitro Cytotoxicity and ADMET Profile

Authors:

Nesrin Karabatak | Bahar Gök | Yasemin Budama-kılınc

Publisher:

Turkish Chemical Society

Main Topics:

Biochemistry and Cell Biology (Other) | Nanochemistry

Journal:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 3, pp.1181-1196

Document Type:

Article

Publication Date:

8/2024

Document Language:

English

Document ID (Org No.):

2001317694

pISSN:

2149-0120

Keywords:

Abstract:

 Ultraviolet UV rays damage DNA, causing adverse effects such as photoaging and cancer on the skin. For the well-being of individuals, there is a need to develop innovative skin products with high effectiveness using protective and therapeutic agents. In this context, in our study, A nanoemulsion NE formulation containing Ylang-ylang essential oil YO, which has many biological active properties such as antimicrobial, antioxidant, anti-inflammatory, and anticancer, was produced by the ultrasonic emulsification method and characterized. The thermodynamic stability was evaluated, and its in vitro release profile determined the dialysis membrane technique. The cytotoxic effect of YO-NE was examined with the in vitro method on the HacaT cell line using the MTT method and in silico method using the ADMET profile. Dynamic light scattering DLS results showed that the average droplet size of the YO-NE formulation was 184.12.307 nm, the polydispersity index PdI was 0.1510.006, and the Zeta potential ζ -10.8  0.400 mV. As a result of in vitro release studies, it was observed that 99.98 1.00 of YO release from NE occurred within 5 hours. Based on the thermodynamic stability test results, it was determined that the developed formulation did not show sedimentation or phase separation. Also, it remained stable under three different storage conditions in the three-month stability test. Cytotoxicity results revealed that the YO-NE formulation was safe. All the results indicated that the YO-NE formulation might be considered a non-toxic product candidate with physicochemical properties suitable for topical use.

 Ultraviolet UV rays damage DNA, causing adverse effects such as photoaging and cancer on the skin. For the well-being of individuals, there is a need to develop innovative skin products with high effectiveness using protective and therapeutic agents. In this context, in our study, A nanoemulsion NE formulation containing Ylang-ylang essential oil YO, which has many biological active properties such as antimicrobial, antioxidant, anti-inflammatory, and anticancer, was produced by the ultrasonic emulsification method and characterized. The thermodynamic stability was evaluated, and its in vitro release profile determined the dialysis membrane technique. The cytotoxic effect of YO-NE was examined with the in vitro method on the HacaT cell line using the MTT method and in silico method using the ADMET profile. Dynamic light scattering DLS results showed that the average droplet size of the YO-NE formulation was 184.12.307 nm, the polydispersity index PdI was 0.1510.006, and the Zeta potential ζ -10.8  0.400 mV. As a result of in vitro release studies, it was observed that 99.98 1.00 of YO release from NE occurred within 5 hours. Based on the thermodynamic stability test results, it was determined that the developed formulation did not show sedimentation or phase separation. Also, it remained stable under three different storage conditions in the three-month stability test. Cytotoxicity results revealed that the YO-NE formulation was safe. All the results indicated that the YO-NE formulation might be considered a non-toxic product candidate with physicochemical properties suitable for topical use.

 Ultraviolet UV rays damage DNA, causing adverse effects such as photoaging and cancer on the skin. For the well-being of individuals, there is a need to develop innovative skin products with high effectiveness using protective and therapeutic agents. In this context, in our study, A nanoemulsion NE formulation containing Ylang-ylang essential oil YO, which has many biological active properties such as antimicrobial, antioxidant, anti-inflammatory, and anticancer, was produced by the ultrasonic emulsification method and characterized. The thermodynamic stability was evaluated, and its in vitro release profile determined the dialysis membrane technique. The cytotoxic effect of YO-NE was examined with the in vitro method on the HacaT cell line using the MTT method and in silico method using the ADMET profile. Dynamic light scattering DLS results showed that the average droplet size of the YO-NE formulation was 184.12.307 nm, the polydispersity index PdI was 0.1510.006, and the Zeta potential ζ -10.8  0.400 mV. As a result of in vitro release studies, it was observed that 99.98 1.00 of YO release from NE occurred within 5 hours. Based on the thermodynamic stability test results, it was determined that the developed formulation did not show sedimentation or phase separation. Also, it remained stable under three different storage conditions in the three-month stability test. Cytotoxicity results revealed that the YO-NE formulation was safe. All the results indicated that the YO-NE formulation might be considered a non-toxic product candidate with physicochemical properties suitable for topical use.

 Ultraviolet UV rays damage DNA, causing adverse effects such as photoaging and cancer on the skin. For the well-being of individuals, there is a need to develop innovative skin products with high effectiveness using protective and therapeutic agents. In this context, in our study, A nanoemulsion NE formulation containing Ylang-ylang essential oil YO, which has many biological active properties such as antimicrobial, antioxidant, anti-inflammatory, and anticancer, was produced by the ultrasonic emulsification method and characterized. The thermodynamic stability was evaluated, and its in vitro release profile determined the dialysis membrane technique. The cytotoxic effect of YO-NE was examined with the in vitro method on the HacaT cell line using the MTT method and in silico method using the ADMET profile. Dynamic light scattering DLS results showed that the average droplet size of the YO-NE formulation was 184.12.307 nm, the polydispersity index PdI was 0.1510.006, and the Zeta potential ζ -10.8  0.400 mV. As a result of in vitro release studies, it was observed that 99.98 1.00 of YO release from NE occurred within 5 hours. Based on the thermodynamic stability test results, it was determined that the developed formulation did not show sedimentation or phase separation. Also, it remained stable under three different storage conditions in the three-month stability test. Cytotoxicity results revealed that the YO-NE formulation was safe. All the results indicated that the YO-NE formulation might be considered a non-toxic product candidate with physicochemical properties suitable for topical use.

Article Details

Development of Nanoemulsion Formulation Containing Ylang Ylang Essential Oil for Topical Applications, Evaluation of In Vitro Cytotoxicity and ADMET Profile

Authors: Nesrin Karabatak | Bahar Gök | Yasemin Budama-kılınc

Publisher: Turkish Chemical Society

Main Topics: Biochemistry and Cell Biology (Other) | Nanochemistry

Journal: Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 3, pp.1181-1196

Document Type: Article

Publication Date: 8/2024

Document Language: English

Document ID (Org No.): 2001317694

pISSN: 2149-0120

Nanoemulsion

Ylang Ylang Essential Oil

Cytotoxicity

Topical Application

Issues

Authors:

Nesrin Karabatak | Bahar Gök | Yasemin Budama-kılınc

Publisher:

Turkish Chemical Society

Main Topics:

Biochemistry and Cell Biology (Other) | Nanochemistry

Journal:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 3, pp.1181-1196

Document Type:

Article

Publication Date:

8/2024

Document Language:

English

Document ID (Org No.):

2001317694

pISSN:

2149-0120