Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin

Nuray GÜY

doi:https://doi.org/10.18466/cbayarfbe.453636

Article Details

Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin

Authors:

Nuray GÜY

Publisher:

Celal Bayar University

Main Topics:

Applied physics

Journal:

Celal Bayar University Journal of Science 2018 Vol.14 Issue 4, pp.449-454

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000706183

Frequency:

Quarterly

eISSN:

1305-1385

pISSN:

1305-130X

DOI:

https://doi.org/10.18466/cbayarfbe.453636

Abstract:

 


NiFe2O4
magnetic nanoparticles were solvothermally prepared. NiFe2O4
magnetic nanoparticles were functionalized with gallic acid and then papain
immobilized on NiFe2O4 functionalized with gallic acid. The obtained samples were
characterized and the activities of free and immobilized papain were studied.
Immobilized papain showed higher and more effective activity than free papain. This
immobilized papain retained about 75.5% of its initial activity after 8 weeks
of storage at 4 °C in sodium phosphate buffer (0.1 M, pH 6.5), while the free
trypsin protected 30.5% of its initial activity during the same condition.
Furthermore, the immobilized papain protected approximately 51% of its initial
activity following the times of ten sequential reuse. Finally, the microwave
assisted digestion of bovine serum albumin was carried out for 15 s with
matrix-assisted laser desorption/ionization mass spectrometry by using free and
immobilized papain.


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Article Details

Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin

Authors: Nuray GÜY

Publisher: Celal Bayar University

Main Topics: Applied physics

Journal: Celal Bayar University Journal of Science 2018 Vol.14 Issue 4, pp.449-454

Document Type: Article

Publication Date: 12/2018

Document Language: English

Document ID (Org No.): 2000706183

Frequency: Quarterly

eISSN: 1305-1385

pISSN: 1305-130X

DOI: https://doi.org/10.18466/cbayarfbe.453636

Issues

Authors:

Nuray GÜY

Publisher:

Celal Bayar University

Main Topics:

Applied physics

Journal:

Celal Bayar University Journal of Science 2018 Vol.14 Issue 4, pp.449-454

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000706183

Frequency:

Quarterly

eISSN:

1305-1385

pISSN:

1305-130X

DOI:

https://doi.org/10.18466/cbayarfbe.453636