Validated HPTLC method for the quantitation of levetiracetam in pure and tablet dosage form

likitha T G; Jose Gnana Babu C.; Sowmya H G.

doi:10.14419/ijac.v8i1.30542

Article Details

Validated HPTLC method for the quantitation of levetiracetam in pure and tablet dosage form

Authors:

likitha T G | Jose Gnana Babu C. | Sowmya H G.

Publisher:

Science Publishing Corporation

Journal:

International Journal of Advanced Chemistry Vol.8 Issue 1, pp.170-174

Document Type:

Article

Publication Date:

5/2020

Document Language:

English

Document ID (Org No.):

2001385732

pISSN:

2310-2977

DOI:

10.14419/ijac.v8i1.30542

Keywords:

Levetiracetam

Abstract:

 A rapid, simple, precise and accurate high performance thin layer chromatographic method has been developed and validated for the estima-tion of Levetiracetam in bulk and tablet dosage form. The Levetiracetam was chromatographed on silica gel 60 F254 HPTLC plate as a sta-tionary phase. The mobile phase was Ethyl acetate: Methanol: Ammonia in the ratio of 7:1:2 respectively. It gave a dense and compact spot of Levetiracetam with an Rf value of 0.56. The quantitation was carried out at 210 nm. The method was validated in terms of linearity, accu-racy, precision and specificity. The statistical analysis proved that the developed method is accurate and reproducible with linearity in the range of 100 to 500 ng/spot. The limit of detection and limit of quantitation for Levetiracetam were 3.55 and 10.66 ng/spot respectively. The developed method can be employed for the routine analysis of Levetiracetam in the tablet dosage form.

Article Details

Validated HPTLC method for the quantitation of levetiracetam in pure and tablet dosage form

Authors: likitha T G | Jose Gnana Babu C. | Sowmya H G.

Publisher: Science Publishing Corporation

Journal: International Journal of Advanced Chemistry Vol.8 Issue 1, pp.170-174

Document Type: Article

Publication Date: 5/2020

Document Language: English

Document ID (Org No.): 2001385732

pISSN: 2310-2977

DOI: 10.14419/ijac.v8i1.30542

Levetiracetam

Issues

Authors:

likitha T G | Jose Gnana Babu C. | Sowmya H G.

Publisher:

Science Publishing Corporation

Journal:

International Journal of Advanced Chemistry Vol.8 Issue 1, pp.170-174

Document Type:

Article

Publication Date:

5/2020

Document Language:

English

Document ID (Org No.):

2001385732

pISSN:

2310-2977

DOI:

10.14419/ijac.v8i1.30542