An Immunoelectronmicroscopic Study on The Pancreatic B-Cells of Mouse

Aysel Seftalioglu

Article Details

An Immunoelectronmicroscopic Study on The Pancreatic B-Cells of Mouse

Authors:

Aysel Seftalioglu

Publisher:

Islamic World Academy of Sciences (IAS)

Journal:

Medical Journal of Islamic World Academy of Sciences Vol.6 Issue 4, pp.262-267

Document Type:

Article

Publication Date:

1/1993

Document Language:

English

Document ID (Org No.):

2001937474

pISSN:

2415-1300

Keywords:

Abstract:

 Recently developed protein A-gold technique for detection of intracellular antigenic sites on thin sections was used to localize a secretory protein, insulin in the pancreatic B-cells of mouse. Treatment of thin sections of glutaraldehyde-fixed, osmium tetroxide-postfixed pancreatic tissue with saturated aqueous solution of sodium metaperiodate, followed by incubation with anti-insulin antibody and protein A-gold complex, restored the labeling without altering the structural preservation. The good ultrastructural preservation obtained led to superior resolution in the labeling. Pancreatic secretory protein, insulin was observed over perfectly preserved secretory granules of the pancreatic B-cells. The protein A-gold technique is proposed as general method for visualization of antigenic sites on thin sections.

Article Details

An Immunoelectronmicroscopic Study on The Pancreatic B-Cells of Mouse

Authors:

Aysel Seftalioglu

Publisher:

Islamic World Academy of Sciences (IAS)

Journal:

Medical Journal of Islamic World Academy of Sciences Vol.6 Issue 4, pp.262-267

Document Type:

Article

Publication Date:

1/1993

Document Language:

English

Document ID (Org No.):

2001937474

pISSN:

2415-1300

Protein A-gold

pancreatic B-cell

antigenic sites

Issues

Article Details

An Immunoelectronmicroscopic Study on The Pancreatic B-Cells of Mouse

Authors: Aysel Seftalioglu

Publisher: Islamic World Academy of Sciences (IAS)

Journal: Medical Journal of Islamic World Academy of Sciences Vol.6 Issue 4, pp.262-267

Document Type: Article

Publication Date: 1/1993

Document Language: English

Document ID (Org No.): 2001937474

pISSN: 2415-1300

Protein A-gold

pancreatic B-cell

antigenic sites

Issues

Authors:

Aysel Seftalioglu

Publisher:

Islamic World Academy of Sciences (IAS)

Journal:

Medical Journal of Islamic World Academy of Sciences Vol.6 Issue 4, pp.262-267

Document Type:

Article

Publication Date:

1/1993

Document Language:

English

Document ID (Org No.):

2001937474

pISSN:

2415-1300