Plasma expression and methylation levels of vascular endothelial growth factor (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia in Çukurova Region, Turkey

Nihal İNANDIKLIOGLU; Osman DEMİRHAN; İbrahim BAYRAM; Atila TANYELİ

doi:https://doi.org/10.17826/cumj.676515

Article Details

Plasma expression and methylation levels of vascular endothelial growth factor (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia in Çukurova Region, Turkey

Authors:

Nihal İNANDIKLIOGLU | Osman DEMİRHAN | İbrahim BAYRAM | Atila TANYELİ

Publisher:

Cukurova University

Main Topics:

Diseases

Journal:

Cukurova Medical Journal 2020 Vol.45 Issue 2, pp.581-587

Document Type:

Article

Publication Date:

6/2020

Document Language:

English

Document ID (Org No.):

2000915068

Frequency:

Quarterly

eISSN:

2602-3040

pISSN:

2602-3032

DOI:

https://doi.org/10.17826/cumj.676515

Keywords:

Abstract:

 


Purpose: The aim of this study was to determine the expression and methylation levels of vascular endothelial growth factor-C (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia (ALL).Materials and Methods: We detected mRNA expression profiles and methylation levels of VEGF-C and bFGF genes in the peripheral blood samples of 26 patients with newly diagnosed ALL and 26 healthy controls by real-time quantitative PCR. Results: The expression level of bFGF was significantly increased (4.23-fold) in ALL patients as compared with controls. Moreover, VEGF-C were significantly decreased (3.41-fold) in ALL patients as compared with controls. The methylation of the promoter region of VEGF-C (6.88%) and bFGF (16.64%) genes was higher in ALL patients than in healthy controls. Conclusion: Long-term changes in VEGF-C and bFGF at different time intervals as a result of years of follow-up of patients may show stronger associations with disease risk. Further studies are required to establish strong links with both VEGF-C and bFGF, and ALL risk factors. 

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Amaç: Bu çalışmanın amacı akut lenfoblastik lösemili (ALL) çocuklarda vasküler endotel büyüme faktörü-C (VEGF-C) ve temel fibroblast büyüme faktörünün (bFGF) ekspresyon ve metilasyon düzeylerini belirlemekti.Gereç ve Yöntem: Gerçek zamanlı kantitatif PCR ile yeni tanı almış olan 26 hasta ve 26 sağlıklı kontrolün periferik kan örneklerinde VEGF-C ve bFGF genlerinin mRNA ekspresyon profilleri ve metilasyon seviyelerini tespit ettik.Bulgular: bFGF ekspresyon seviyesi ALL hastalarında kontrollere kıyasla anlamlı derecede artmıştı (4.23 kat). Ayrıca, VEGF-C ALL hastalarında kontrollere kıyasla anlamlı derecede azalmıştı (3.41 kat). VEGF-C (%6.88) ve bFGF (%16.64) genlerinin promotor bölgelerinin metilasyon oranı ALL hastalarında sağlıklı kontroller göre daha yüksekti.Sonuç: Hastaların yıllar süren takibi sonucunda VEGF-C ve bFGF'de farklı zaman aralıklarında uzun süreli değişiklikler hastalık riski ile daha güçlü ilişkiler gösterebilir. Hem VEGF-C hem de bFGF ve ALL risk faktörleri ile güçlü bağlantılar kurmak için daha ileri çalışmalara ihtiyaç vardır.

Article Details

Plasma expression and methylation levels of vascular endothelial growth factor (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia in Çukurova Region, Turkey

Authors:

Nihal İNANDIKLIOGLU | Osman DEMİRHAN | İbrahim BAYRAM | Atila TANYELİ

Publisher:

Cukurova University

Main Topics:

Diseases

Journal:

Cukurova Medical Journal 2020 Vol.45 Issue 2, pp.581-587

Document Type:

Article

Publication Date:

6/2020

Document Language:

English

Document ID (Org No.):

2000915068

Frequency:

Quarterly

eISSN:

2602-3040

pISSN:

2602-3032

DOI:

https://doi.org/10.17826/cumj.676515

acute lymphoblastic leukemia

basic fibroblast growth factor

gene expression

methylation

vascular endothelial growth factor

Issues

Article Details

Plasma expression and methylation levels of vascular endothelial growth factor (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia in Çukurova Region, Turkey

Authors: Nihal İNANDIKLIOGLU | Osman DEMİRHAN | İbrahim BAYRAM | Atila TANYELİ

Publisher: Cukurova University

Main Topics: Diseases

Journal: Cukurova Medical Journal 2020 Vol.45 Issue 2, pp.581-587

Document Type: Article

Publication Date: 6/2020

Document Language: English

Document ID (Org No.): 2000915068

Frequency: Quarterly

eISSN: 2602-3040

pISSN: 2602-3032

DOI: https://doi.org/10.17826/cumj.676515

acute lymphoblastic leukemia

basic fibroblast growth factor

gene expression

methylation

vascular endothelial growth factor

Issues

Authors:

Nihal İNANDIKLIOGLU | Osman DEMİRHAN | İbrahim BAYRAM | Atila TANYELİ

Publisher:

Cukurova University

Main Topics:

Diseases

Journal:

Cukurova Medical Journal 2020 Vol.45 Issue 2, pp.581-587

Document Type:

Article

Publication Date:

6/2020

Document Language:

English

Document ID (Org No.):

2000915068

Frequency:

Quarterly

eISSN:

2602-3040

pISSN:

2602-3032

DOI:

https://doi.org/10.17826/cumj.676515