Phylogenetic characterization of Cochroaches (Insecta: Blattaria) in Türkiye and determination of their vector potential for medically important parasites

Fatma Cevahir; Önder Düzlü; Mübeccel Atelge; Alparslan Yıldırım

Article Details

Phylogenetic characterization of Cochroaches (Insecta: Blattaria) in Türkiye and determination of their vector potential for medically important parasites

Authors:

Fatma Cevahir | Önder Düzlü | Mübeccel Atelge | Alparslan Yıldırım

Publisher:

Ankara University

Main Topics:

Veterinary Parasitology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.417-426

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001325152

pISSN:

1300-0861

Keywords:

Abstract:

 This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I mt-COI, mt-COII, and internal transcribed spacer-2 ITS-2 gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism RFLP was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species n220 collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica n105, Blatta orientalis n86, and Periplaneta americana n29. A total of 13 haplotypes were detected and maximum likelihood ML analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 79.3 belonged to helminth species and 12 20.7 to protozoan species. The results showed that B. germanica 58.6 had the highest prevalence, followed by Bl. orientalis 32.8 and P. americana 8.6. The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.

 This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I mt-COI, mt-COII, and internal transcribed spacer-2 ITS-2 gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism RFLP was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species n220 collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica n105, Blatta orientalis n86, and Periplaneta americana n29. A total of 13 haplotypes were detected and maximum likelihood ML analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 79.3 belonged to helminth species and 12 20.7 to protozoan species. The results showed that B. germanica 58.6 had the highest prevalence, followed by Bl. orientalis 32.8 and P. americana 8.6. The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.

 This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I mt-COI, mt-COII, and internal transcribed spacer-2 ITS-2 gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism RFLP was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species n220 collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica n105, Blatta orientalis n86, and Periplaneta americana n29. A total of 13 haplotypes were detected and maximum likelihood ML analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 79.3 belonged to helminth species and 12 20.7 to protozoan species. The results showed that B. germanica 58.6 had the highest prevalence, followed by Bl. orientalis 32.8 and P. americana 8.6. The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.

 This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I mt-COI, mt-COII, and internal transcribed spacer-2 ITS-2 gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism RFLP was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species n220 collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica n105, Blatta orientalis n86, and Periplaneta americana n29. A total of 13 haplotypes were detected and maximum likelihood ML analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 79.3 belonged to helminth species and 12 20.7 to protozoan species. The results showed that B. germanica 58.6 had the highest prevalence, followed by Bl. orientalis 32.8 and P. americana 8.6. The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.

Article Details

Phylogenetic characterization of Cochroaches (Insecta: Blattaria) in Türkiye and determination of their vector potential for medically important parasites

Authors:

Fatma Cevahir | Önder Düzlü | Mübeccel Atelge | Alparslan Yıldırım

Publisher:

Ankara University

Main Topics:

Veterinary Parasitology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.417-426

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001325152

pISSN:

1300-0861

Cockroach

DNA barcoding

ITS-2

mt‐COI and mt‐COII

Vector

Issues

Article Details

Phylogenetic characterization of Cochroaches (Insecta: Blattaria) in Türkiye and determination of their vector potential for medically important parasites

Authors: Fatma Cevahir | Önder Düzlü | Mübeccel Atelge | Alparslan Yıldırım

Publisher: Ankara University

Main Topics: Veterinary Parasitology

Journal: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.417-426

Document Type: Article

Publication Date: 9/2024

Document Language: English

Document ID (Org No.): 2001325152

pISSN: 1300-0861

Cockroach

DNA barcoding

ITS-2

mt‐COI and mt‐COII

Vector

Issues

Authors:

Fatma Cevahir | Önder Düzlü | Mübeccel Atelge | Alparslan Yıldırım

Publisher:

Ankara University

Main Topics:

Veterinary Parasitology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.417-426

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001325152

pISSN:

1300-0861