Micropropagation and Acclimatization of Large Cardamom (Amomum subulatum Roxb.)

Krishna POUDEL; Hari Kumar PRASAI; Jiban SHRESTHA

doi:https://doi.org/10.30910/turkjans.448318

Article Details

Micropropagation and Acclimatization of Large Cardamom (Amomum subulatum Roxb.)

Authors:

Krishna POUDEL | Hari Kumar PRASAI | Jiban SHRESTHA

Publisher:

Mevlüt AKÇURA

Journal:

Turkish Journal of Agricultural and Natural Sciences 2018 Vol.5 Issue 3, pp.231-235

Document Type:

Article

Publication Date:

7/2018

Document Language:

English

Document ID (Org No.):

2000669932

Frequency:

Quarterly

pISSN:

2148-3647

DOI:

https://doi.org/10.30910/turkjans.448318

Abstract:

 


In vitro clonal propagation
and acclimatization of the tissue culture plant of large
cardamom has been established at Agriculture Research Station Pakhribas,
Dhankuta, Nepal. The rhizome buds were collected from the screen house and then
cultured in Murashige and Skoog (MS) medium enriched with 9 different
concentrations of BAP (6-benzylaminopurine) and IBA (indole-3-butyric acid)
namely 0.5 mg/L (BAP), 1.0 mg/L (BAP), 1.5 mg/L (BAP), 2.0 mg/L (BAP),  1 mg/L (IBA), 0.5 mg/L (BAP)+ 1 mg/L (IBA),
1.0 mg/L (BAP)+ 1 mg/L (IBA), 1.5 mg/L (BAP)+ 1 mg/L (IBA) and 2.0 mg/L (BAP)+
1 mg/L (IBA). This study showed that the explants grown in MS medium
supplemented with 1.0 mg / L BAP + 1.0 mg / L IBA showed the maximum root
induction rate. Buds produced roots in the same medium. The roots planted in
the screen house were transplanted for the hardening process. Then these
hardened plants were transferred to the netted nursery for further
multiplication process. This protocol developed could be used for developing
the superior quality plants of large cardamom.

.

 


In vitro clonal propagation
and acclimatization of the tissue culture plant of large
cardamom has been established at Agriculture Research Station Pakhribas,
Dhankuta, Nepal. The rhizome buds were collected from the screen house and then
cultured in Murashige and Skoog (MS) medium enriched with 9 different
concentrations of BAP (6-benzylaminopurine) and IBA (indole-3-butyric acid)
namely 0.5 mg/L (BAP), 1.0 mg/L (BAP), 1.5 mg/L (BAP), 2.0 mg/L (BAP),  1 mg/L (IBA), 0.5 mg/L (BAP)+ 1 mg/L (IBA),
1.0 mg/L (BAP)+ 1 mg/L (IBA), 1.5 mg/L (BAP)+ 1 mg/L (IBA) and 2.0 mg/L (BAP)+
1 mg/L (IBA). This study showed that the explants grown in MS medium
supplemented with 1.0 mg / L BAP + 1.0 mg / L IBA showed the maximum root
induction rate. Buds produced roots in the same medium. The roots planted in
the screen house were transplanted for the hardening process. Then these
hardened plants were transferred to the netted nursery for further
multiplication process. This protocol developed could be used for developing
the superior quality plants of large cardamom.

.

Article Details

Micropropagation and Acclimatization of Large Cardamom (Amomum subulatum Roxb.)

Authors: Krishna POUDEL | Hari Kumar PRASAI | Jiban SHRESTHA

Publisher: Mevlüt AKÇURA

Journal: Turkish Journal of Agricultural and Natural Sciences 2018 Vol.5 Issue 3, pp.231-235

Document Type: Article

Publication Date: 7/2018

Document Language: English

Document ID (Org No.): 2000669932

Frequency: Quarterly

pISSN: 2148-3647

DOI: https://doi.org/10.30910/turkjans.448318

Issues

Authors:

Krishna POUDEL | Hari Kumar PRASAI | Jiban SHRESTHA

Publisher:

Mevlüt AKÇURA

Journal:

Turkish Journal of Agricultural and Natural Sciences 2018 Vol.5 Issue 3, pp.231-235

Document Type:

Article

Publication Date:

7/2018

Document Language:

English

Document ID (Org No.):

2000669932

Frequency:

Quarterly

pISSN:

2148-3647

DOI:

https://doi.org/10.30910/turkjans.448318