Evaluation of Different Methods of Curing Bacterial Plasmids

Dalal. M.R. Al-Shuailiyah; Zahraa M. AL-Taee; Raflaa S.H. Hussian; Hawraa Mohammed Ridha; Rasha Nayyef Ali

Article Details

Evaluation of Different Methods of Curing Bacterial Plasmids

Authors:

Dalal. M.R. Al-Shuailiyah | Zahraa M. AL-Taee | Raflaa S.H. Hussian | Hawraa Mohammed Ridha | Rasha Nayyef Ali

Publisher:

University of Babylon

Journal:

JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences Vol.28 Issue 3, pp.55-67

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001382953

pISSN:

2312-8135

Keywords:

Abstract:

 Plasmids play an important role in the spread of antibiotic resistance among bacterial strains that pose a threat to global health. Traditional methods of curing plasmid rely on the development of bacterial strains under inappropriate conditions, such as high temperature or the addition of intruding agents that interfere with the structure of DNA synthesis during the replication of plasmids. Other methods rely on the phenomenon of plasmid compatibility based on the principle of competition between identical plasmids but require accurate knowledge of the mechanism of multiplying the target plasmid, in addition to the subsequent treatment of the interfering plasmid. With the advent of Crispras9, which simulates natural bacterial defense against plasmid and latex infiltrators, this technique has been used as an accurate tool for targeting places specializing in plasmid DNA. In this review, we will address the most important methods of removing and deleting plasmids from bacterial cells.

Article Details

Evaluation of Different Methods of Curing Bacterial Plasmids

Authors:

Dalal. M.R. Al-Shuailiyah | Zahraa M. AL-Taee | Raflaa S.H. Hussian | Hawraa Mohammed Ridha | Rasha Nayyef Ali

Publisher:

University of Babylon

Journal:

JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences Vol.28 Issue 3, pp.55-67

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001382953

pISSN:

2312-8135

Plasmid

Type of plasmid

Mechanism the replication of plasmid

curing plasmid

CRISPRcas9

Issues

Article Details

Evaluation of Different Methods of Curing Bacterial Plasmids

Authors: Dalal. M.R. Al-Shuailiyah | Zahraa M. AL-Taee | Raflaa S.H. Hussian | Hawraa Mohammed Ridha | Rasha Nayyef Ali

Publisher: University of Babylon

Journal: JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences Vol.28 Issue 3, pp.55-67

Document Type: Article

Publication Date: 12/2020

Document Language: English

Document ID (Org No.): 2001382953

pISSN: 2312-8135

Plasmid

Type of plasmid

Mechanism the replication of plasmid

curing plasmid

CRISPRcas9

Issues

Authors:

Dalal. M.R. Al-Shuailiyah | Zahraa M. AL-Taee | Raflaa S.H. Hussian | Hawraa Mohammed Ridha | Rasha Nayyef Ali

Publisher:

University of Babylon

Journal:

JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences Vol.28 Issue 3, pp.55-67

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001382953

pISSN:

2312-8135