Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein

Burcu Bayyurt; Serdal Arslan

Article Details

Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein

Authors:

Burcu Bayyurt | Serdal Arslan

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Gen İfadesi

Journal:

Black Sea Journal of Engineering and Science Vol.8 Issue 1, pp.54-57

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001331066

pISSN:

2619-8991

Keywords:

Abstract:

 Oxidized low-density lipoprotein ox-LDL shows many harmful effects such as induction of apoptosis on function of endothelial cell EC. Fas ligand FASL induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells HUVECs with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction qPCR. We compared FASL gene expression level between the groups according to Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL P0.001. We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

 Oxidized low-density lipoprotein ox-LDL shows many harmful effects such as induction of apoptosis on function of endothelial cell EC. Fas ligand FASL induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells HUVECs with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction qPCR. We compared FASL gene expression level between the groups according to Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL P0.001. We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

 Oxidized low-density lipoprotein ox-LDL shows many harmful effects such as induction of apoptosis on function of endothelial cell EC. Fas ligand FASL induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells HUVECs with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction qPCR. We compared FASL gene expression level between the groups according to Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL P0.001. We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

 Oxidized low-density lipoprotein ox-LDL shows many harmful effects such as induction of apoptosis on function of endothelial cell EC. Fas ligand FASL induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells HUVECs with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction qPCR. We compared FASL gene expression level between the groups according to Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL P0.001. We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

Article Details

Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein

Authors:

Burcu Bayyurt | Serdal Arslan

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Gen İfadesi

Journal:

Black Sea Journal of Engineering and Science Vol.8 Issue 1, pp.54-57

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001331066

pISSN:

2619-8991

Oxidized low-density lipoprotein

Fas ligand

Gene expression

Quantitative polymerase chain reaction

Human umbilical vein endothelial cellOxidized low-density lipoprotein

Fas ligand

Gene expression

Quantitative polymerase chain reaction

Human umbilical vein endothelial cell

Issues

Article Details

Authors: Burcu Bayyurt | Serdal Arslan

Publisher: Karyay Karadeniz Yayımcılık

Main Topics: Gen İfadesi

Journal: Black Sea Journal of Engineering and Science Vol.8 Issue 1, pp.54-57

Document Type: Article

Publication Date: 1/2025

Document Language: English

Document ID (Org No.): 2001331066

pISSN: 2619-8991

Issues

Authors:

Burcu Bayyurt | Serdal Arslan

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Gen İfadesi

Journal:

Black Sea Journal of Engineering and Science Vol.8 Issue 1, pp.54-57

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001331066

pISSN:

2619-8991