Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level

Deepali BISHT; Santosh Kumar YADAV; Nandan Singh DARMWAL

Article Details

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level

Authors:

Deepali BISHT | Santosh Kumar YADAV | Nandan Singh DARMWAL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.392-404

Document Type:

Article

Publication Date:

3/2013

Document Language:

Turkish

Document ID (Org No.):

2000812386

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production (4363.4 U/mL), followed by polyacrylamide gel (2172.3 U/mL). Alginate beads were the poorest. The one-variable-at-a-time approach suggested agarose at 2.0%, immobilized bead at 4.0 g blocks/50 mL, and initial cell loading of 0.8 g in the matrix as optimum conditions for maximum lipase production (5982.3 U/mL) after 24 h of incubation. However, response surface methodology studies determined the optimum values of these variables as 1.96%, 4.06 g blocks/50 mL, and 0.81 g of cells in the matrix for maximum lipase production (6354.23 U/mL) within 22.54 h of incubation. The agarose blocks were reusable for 7 cycles without any significant loss in lipase yield. Bench-scale bioreactor level optimization resulted in further enhancement in lipase yield (6815.3 U/mL) at 0.6 vvm aeration and 100 rpm agitation within only 20 h of incubation. Presumably, this is the first attempt for lipase production by immobilized cells of P. aeruginosa at the bioreactor level. The agarose-immobilized mutant cells showed potential candidature for alkaline lipase production at the industrial level.

 

Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production (4363.4 U/mL), followed by polyacrylamide gel (2172.3 U/mL). Alginate beads were the poorest. The one-variable-at-a-time approach suggested agarose at 2.0%, immobilized bead at 4.0 g blocks/50 mL, and initial cell loading of 0.8 g in the matrix as optimum conditions for maximum lipase production (5982.3 U/mL) after 24 h of incubation. However, response surface methodology studies determined the optimum values of these variables as 1.96%, 4.06 g blocks/50 mL, and 0.81 g of cells in the matrix for maximum lipase production (6354.23 U/mL) within 22.54 h of incubation. The agarose blocks were reusable for 7 cycles without any significant loss in lipase yield. Bench-scale bioreactor level optimization resulted in further enhancement in lipase yield (6815.3 U/mL) at 0.6 vvm aeration and 100 rpm agitation within only 20 h of incubation. Presumably, this is the first attempt for lipase production by immobilized cells of P. aeruginosa at the bioreactor level. The agarose-immobilized mutant cells showed potential candidature for alkaline lipase production at the industrial level.

Article Details

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level

Authors:

Deepali BISHT | Santosh Kumar YADAV | Nandan Singh DARMWAL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.392-404

Document Type:

Article

Publication Date:

3/2013

Document Language:

Turkish

Document ID (Org No.):

2000812386

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Alkaline lipase

bench-scale bioreactor

immobilized cells

response surface methodology

Issues

Article Details

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level

Authors: Deepali BISHT | Santosh Kumar YADAV | Nandan Singh DARMWAL

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.392-404

Document Type: Article

Publication Date: 3/2013

Document Language: Turkish

Document ID (Org No.): 2000812386

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Alkaline lipase

bench-scale bioreactor

immobilized cells

response surface methodology

Issues

Authors:

Deepali BISHT | Santosh Kumar YADAV | Nandan Singh DARMWAL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.392-404

Document Type:

Article

Publication Date:

3/2013

Document Language:

Turkish

Document ID (Org No.):

2000812386

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152