Investigation of enterotoxin-encoding genes and antibiotic resistance in Bacillus cereus strains isolated from cheese produced in Malatya province

H. Yeşim Can; Kardelen Banu Sarı

Article Details

Investigation of enterotoxin-encoding genes and antibiotic resistance in Bacillus cereus strains isolated from cheese produced in Malatya province

Authors:

H. Yeşim Can | Kardelen Banu Sarı

Publisher:

Osmaniye Korkut Ata Üniversitesi

Main Topics:

Gıda Mühendisliği

Journal:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1500-1512

Document Type:

Article

Publication Date:

5/2023

Document Language:

Turkish

Document ID (Org No.):

2001295514

pISSN:

2687-3729

Keywords:

Abstract:

 In this study, it was aimed, i to determine the presence of Bacillus cereus in local village type cheese samples produced in Malatya and consumed widely by the people of Malatya, ii detection of enterotoxin genes in the isolates by polymerase chain reaction PCR, and iii determination of antibiotic resistance. B. cereus was detected in 55 73.3 of 75 cheese samples in total. After molecular analysis, a total of 55 isolates were confirmed as B. cereus by targeting the hemolysin gene. It was determined that 3 of these isolates carried only the nhe gene, and 1 of them carried both nhe and cytK genes. However, no hbl gene was found in any of the isolates. As a result of the antibiotic susceptibility test, all of the isolates 100  were resistant to cefoxitin, followed by resistance to rifampin 94.5, penicillin 87.2, erythromycin 58.1, gentamicin 56.3, and chloramphenicol 12.7. It was determined that 94.5 of the isolates showed multiple antibiotic resistance. Within the scope of food safety and public health, these findings can give important information to the both dairy industry and public in order to take the necessary technological and hygienic measures for healthy cheese production. In addition, determination of very high level of multiple antibiotic resistance among the isolates will contribute to the protection of public health depending on the measures to be taken in this regard.

 In this study, it was aimed, i to determine the presence of Bacillus cereus in local village type cheese samples produced in Malatya and consumed widely by the people of Malatya, ii detection of enterotoxin genes in the isolates by polymerase chain reaction PCR, and iii determination of antibiotic resistance. B. cereus was detected in 55 73.3 of 75 cheese samples in total. After molecular analysis, a total of 55 isolates were confirmed as B. cereus by targeting the hemolysin gene. It was determined that 3 of these isolates carried only the nhe gene, and 1 of them carried both nhe and cytK genes. However, no hbl gene was found in any of the isolates. As a result of the antibiotic susceptibility test, all of the isolates 100  were resistant to cefoxitin, followed by resistance to rifampin 94.5, penicillin 87.2, erythromycin 58.1, gentamicin 56.3, and chloramphenicol 12.7. It was determined that 94.5 of the isolates showed multiple antibiotic resistance. Within the scope of food safety and public health, these findings can give important information to the both dairy industry and public in order to take the necessary technological and hygienic measures for healthy cheese production. In addition, determination of very high level of multiple antibiotic resistance among the isolates will contribute to the protection of public health depending on the measures to be taken in this regard.

 In this study, it was aimed, i to determine the presence of Bacillus cereus in local village type cheese samples produced in Malatya and consumed widely by the people of Malatya, ii detection of enterotoxin genes in the isolates by polymerase chain reaction PCR, and iii determination of antibiotic resistance. B. cereus was detected in 55 73.3 of 75 cheese samples in total. After molecular analysis, a total of 55 isolates were confirmed as B. cereus by targeting the hemolysin gene. It was determined that 3 of these isolates carried only the nhe gene, and 1 of them carried both nhe and cytK genes. However, no hbl gene was found in any of the isolates. As a result of the antibiotic susceptibility test, all of the isolates 100  were resistant to cefoxitin, followed by resistance to rifampin 94.5, penicillin 87.2, erythromycin 58.1, gentamicin 56.3, and chloramphenicol 12.7. It was determined that 94.5 of the isolates showed multiple antibiotic resistance. Within the scope of food safety and public health, these findings can give important information to the both dairy industry and public in order to take the necessary technological and hygienic measures for healthy cheese production. In addition, determination of very high level of multiple antibiotic resistance among the isolates will contribute to the protection of public health depending on the measures to be taken in this regard.

 Bu çalışmada, Malatya ilinde üretilen ve halk tarafından yaygın olarak tüketilen yöresel köy tipi peynir örneklerinde, i Bacillus cereusun varlığının belirlenmesi, ii izolatlarda enterotoksin genlerinin polimeraz zincir reaksiyonu PCR ile saptanması ve iii antibiyotik direncinin belirlenmesi amaçlandı. Analize alınan toplam 75 adet peynir örneğinin 55inde 73,3 B. cereus saptandı. Yapılan moleküler analizler sonucunda, toplam 55 adet izolat B. cereus yönünden hemolizin geni hedef alınarak doğrulandı. Bu izolatlardan 3ünün sadece nhe genini, 1inin ise hem nhe hem de cytK genlerini taşıdığı tespit edilirken, ancak izolatların hiçbirisinde hbl genine rastlanılmadı. Antibiyotik duyarlılık testi sonucunda, izolatların tamamı 100 sefoksitine karşı dirençli olarak bulundu. Bunu sırasıyla, 94,5 ile rifampin, 87,2 ile penisilin, 58,1 ile eritromisin, 56,3 ile gentamisin ve 12,7 ile kloramfenikol izledi. İzolatların 94,5inin çoklu antibiyotik direnci gösterdiği belirlendi. Gıda güvenliği ve halk sağlığı kapsamında değerlendirildiğinde, çalışmadan elde edilen bulgular, sağlıklı peynir üretimi için gerekli teknolojik ve hijyenik önlemlerin alınması yönünde hem süt endüstrisine hem de halka önemli bilgiler sunmaktadır. Ayrıca, izolatların yüksek düzeyde çoklu antibiyotik direnci göstermesi, bu konuda alınacak önlemlere bağlı olarak halk sağlığının korunmasına katkı sağlayacaktır.

Article Details

Investigation of enterotoxin-encoding genes and antibiotic resistance in Bacillus cereus strains isolated from cheese produced in Malatya province

Authors:

H. Yeşim Can | Kardelen Banu Sarı

Publisher:

Osmaniye Korkut Ata Üniversitesi

Main Topics:

Gıda Mühendisliği

Journal:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1500-1512

Document Type:

Article

Publication Date:

5/2023

Document Language:

Turkish

Document ID (Org No.):

2001295514

pISSN:

2687-3729

Bacillus cereus

cheese

antibiotic

enterotoxin

geneBacillus cereus

peynir

antibiyotik

enterotoksin

gen

Issues

Article Details

Authors: H. Yeşim Can | Kardelen Banu Sarı

Publisher: Osmaniye Korkut Ata Üniversitesi

Main Topics: Gıda Mühendisliği

Journal: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1500-1512

Document Type: Article

Publication Date: 5/2023

Document Language: Turkish

Document ID (Org No.): 2001295514

pISSN: 2687-3729

Issues

Authors:

H. Yeşim Can | Kardelen Banu Sarı

Publisher:

Osmaniye Korkut Ata Üniversitesi

Main Topics:

Gıda Mühendisliği

Journal:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1500-1512

Document Type:

Article

Publication Date:

5/2023

Document Language:

Turkish

Document ID (Org No.):

2001295514

pISSN:

2687-3729