The Comparative Molecular Typing of Haemophilus Influenzae Strains Isolated from the Adenoid and Tonsils in Patients Undergoing Adenotonsillectomy

Gülşen Günel; Levent Aydemir; Özlem Ünaldı; Yasar Nakipoğlu

Article Details

The Comparative Molecular Typing of Haemophilus Influenzae Strains Isolated from the Adenoid and Tonsils in Patients Undergoing Adenotonsillectomy

Authors:

Gülşen Günel | Levent Aydemir | Özlem Ünaldı | Yasar Nakipoğlu

Publisher:

Istanbul University

Main Topics:

Clinical Sciences

Journal:

Experimed Vol.13 Issue 1, pp.39-44

Document Type:

Article

Publication Date:

11/2023

Document Language:

English

Document ID (Org No.):

2001289675

pISSN:

2667-5846

Keywords:

Abstract:

 Objective: Microbiota in the upper respiratory tract begins to form immediately after birth. Aerobic and anaerobic flora will form when a healthy child reaches the age of five. Tonsillitis and adenoiditis are the most common upper respiratory tract infectious diseases in childhood and adulthood. Transmission of bacterial infections from tonsils to adenoid tissue endogenous transmission can be prevented by usage of suitable antibiotics. At the same time, continuous exogenous infections of each or both sites require adenotonsillectomy surgery. In this study, to understand the role of endogenous transmission of Haemophilus influenzae H. influenzae in adenotonsillectomy, we aimed to investigate the genetic relatedness of H. influenzae strains obtained from the tonsil and adenoid of the same patient. Materials and Methods: Twenty eight patients were included the study for displaying the growth of 56 H. influenzae strains 28 isolates per site. We investigated the genetic relatedness of H. influenzae strains obtained from the tonsils and adenoids of the patients by using the pulsed-field gel electrophoresis PFGE method. Results: Twenty one isolates were isolated from the tonsils and adenoids of unrelated patients. We excluded nine 32.2 out of 28 patients H. influenzae isolates from the study, that had identical strains depending on the 80 similarity Dice coefficient. Conclusion: The probability of endogenous transmission between the two sites was very high, which means that some adenotonsillectomy surgery might be avoided and treated with antibiotics.

 Objective: Microbiota in the upper respiratory tract begins to form immediately after birth. Aerobic and anaerobic flora will form when a healthy child reaches the age of five. Tonsillitis and adenoiditis are the most common upper respiratory tract infectious diseases in childhood and adulthood. Transmission of bacterial infections from tonsils to adenoid tissue endogenous transmission can be prevented by usage of suitable antibiotics. At the same time, continuous exogenous infections of each or both sites require adenotonsillectomy surgery. In this study, to understand the role of endogenous transmission of Haemophilus influenzae H. influenzae in adenotonsillectomy, we aimed to investigate the genetic relatedness of H. influenzae strains obtained from the tonsil and adenoid of the same patient. Materials and Methods: Twenty eight patients were included the study for displaying the growth of 56 H. influenzae strains 28 isolates per site. We investigated the genetic relatedness of H. influenzae strains obtained from the tonsils and adenoids of the patients by using the pulsed-field gel electrophoresis PFGE method. Results: Twenty one isolates were isolated from the tonsils and adenoids of unrelated patients. We excluded nine 32.2 out of 28 patients H. influenzae isolates from the study, that had identical strains depending on the 80 similarity Dice coefficient. Conclusion: The probability of endogenous transmission between the two sites was very high, which means that some adenotonsillectomy surgery might be avoided and treated with antibiotics.

 Objective: Microbiota in the upper respiratory tract begins to form immediately after birth. Aerobic and anaerobic flora will form when a healthy child reaches the age of five. Tonsillitis and adenoiditis are the most common upper respiratory tract infectious diseases in childhood and adulthood. Transmission of bacterial infections from tonsils to adenoid tissue endogenous transmission can be prevented by usage of suitable antibiotics. At the same time, continuous exogenous infections of each or both sites require adenotonsillectomy surgery. In this study, to understand the role of endogenous transmission of Haemophilus influenzae H. influenzae in adenotonsillectomy, we aimed to investigate the genetic relatedness of H. influenzae strains obtained from the tonsil and adenoid of the same patient. Materials and Methods: Twenty eight patients were included the study for displaying the growth of 56 H. influenzae strains 28 isolates per site. We investigated the genetic relatedness of H. influenzae strains obtained from the tonsils and adenoids of the patients by using the pulsed-field gel electrophoresis PFGE method. Results: Twenty one isolates were isolated from the tonsils and adenoids of unrelated patients. We excluded nine 32.2 out of 28 patients H. influenzae isolates from the study, that had identical strains depending on the 80 similarity Dice coefficient. Conclusion: The probability of endogenous transmission between the two sites was very high, which means that some adenotonsillectomy surgery might be avoided and treated with antibiotics.

 Objective: Microbiota in the upper respiratory tract begins to form immediately after birth. Aerobic and anaerobic flora will form when a healthy child reaches the age of five. Tonsillitis and adenoiditis are the most common upper respiratory tract infectious diseases in childhood and adulthood. Transmission of bacterial infections from tonsils to adenoid tissue endogenous transmission can be prevented by usage of suitable antibiotics. At the same time, continuous exogenous infections of each or both sites require adenotonsillectomy surgery. In this study, to understand the role of endogenous transmission of Haemophilus influenzae H. influenzae in adenotonsillectomy, we aimed to investigate the genetic relatedness of H. influenzae strains obtained from the tonsil and adenoid of the same patient. Materials and Methods: Twenty eight patients were included the study for displaying the growth of 56 H. influenzae strains 28 isolates per site. We investigated the genetic relatedness of H. influenzae strains obtained from the tonsils and adenoids of the patients by using the pulsed-field gel electrophoresis PFGE method. Results: Twenty one isolates were isolated from the tonsils and adenoids of unrelated patients. We excluded nine 32.2 out of 28 patients H. influenzae isolates from the study, that had identical strains depending on the 80 similarity Dice coefficient. Conclusion: The probability of endogenous transmission between the two sites was very high, which means that some adenotonsillectomy surgery might be avoided and treated with antibiotics.

Article Details

The Comparative Molecular Typing of Haemophilus Influenzae Strains Isolated from the Adenoid and Tonsils in Patients Undergoing Adenotonsillectomy

Authors:

Gülşen Günel | Levent Aydemir | Özlem Ünaldı | Yasar Nakipoğlu

Publisher:

Istanbul University

Main Topics:

Clinical Sciences

Journal:

Experimed Vol.13 Issue 1, pp.39-44

Document Type:

Article

Publication Date:

11/2023

Document Language:

English

Document ID (Org No.):

2001289675

pISSN:

2667-5846

Adenotonsillectomy

pulse field gel electrophoresis

Haemophilus influenzae

Issues

Article Details

The Comparative Molecular Typing of Haemophilus Influenzae Strains Isolated from the Adenoid and Tonsils in Patients Undergoing Adenotonsillectomy

Authors: Gülşen Günel | Levent Aydemir | Özlem Ünaldı | Yasar Nakipoğlu

Publisher: Istanbul University

Main Topics: Clinical Sciences

Journal: Experimed Vol.13 Issue 1, pp.39-44

Document Type: Article

Publication Date: 11/2023

Document Language: English

Document ID (Org No.): 2001289675

pISSN: 2667-5846

Adenotonsillectomy

pulse field gel electrophoresis

Haemophilus influenzae

Issues

Authors:

Gülşen Günel | Levent Aydemir | Özlem Ünaldı | Yasar Nakipoğlu

Publisher:

Istanbul University

Main Topics:

Clinical Sciences

Journal:

Experimed Vol.13 Issue 1, pp.39-44

Document Type:

Article

Publication Date:

11/2023

Document Language:

English

Document ID (Org No.):

2001289675

pISSN:

2667-5846