Histopathological effects of S. xylosus peptidoglycan in comparison to E. coli lipopolysaccharide in the urinary tract of mice

Harith Jabbar Fahad AL-MATHKHURY; May Talib FLAIH; Hiba Khalil TAWFIQ

Article Details

Histopathological effects of S. xylosus peptidoglycan in comparison to E. coli lipopolysaccharide in the urinary tract of mice

Authors:

Harith Jabbar Fahad AL-MATHKHURY | May Talib FLAIH | Hiba Khalil TAWFIQ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1278-1285

Document Type:

Article

Publication Date:

11/2011

Document Language:

Turkish

Document ID (Org No.):

2000754378

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

The present work, among other previous studies done in our lab, aimed to highlight the histopathological effect of S. xylosus peptidoglycan in comparison to LPS of E. coli.  Materials and methods: One hundred and fifty urine specimens were collected from urinary tract infection patients visiting Baghdad hospitals. The histopathological effects of S. xylosus S24 peptidoglycan was studied in the urinary tract of female mice by injecting 5 animal groups at the following concentrations: 1000, 2000, 3000, 4000, and 5000 µg/mL. Another 5 groups were injected with 10, 25, 50, 75, and 100 ng/mL of E. coli (serotype 0128:B12) LPS.   Results: Ten isolates were confirmed to be Staphylococcus xylosus. Histopathological study showed different pathological changes caused by LPS and peptidoglycan, including shrinkage of glomeruli, congestion of blood vessels, and infiltration of inflammatory cells in the kidney, whereas dekeratinization and edematous dehydration of the epithelium were seen in the urinary bladder. Based on a comparison between histopathological changes caused by LPS and peptidoglycan, LPS induced changes of significantly higher severity than peptidoglycan.   Conclusion: Peptidoglycan of S. xylosus had remarkable histopathological effects on the renal system of mice in a dose-dependent manner. Nevertheless, these effects had less intensity than the effects of LPS of E. coli.

 

The present work, among other previous studies done in our lab, aimed to highlight the histopathological effect of S. xylosus peptidoglycan in comparison to LPS of E. coli.  Materials and methods: One hundred and fifty urine specimens were collected from urinary tract infection patients visiting Baghdad hospitals. The histopathological effects of S. xylosus S24 peptidoglycan was studied in the urinary tract of female mice by injecting 5 animal groups at the following concentrations: 1000, 2000, 3000, 4000, and 5000 µg/mL. Another 5 groups were injected with 10, 25, 50, 75, and 100 ng/mL of E. coli (serotype 0128:B12) LPS.   Results: Ten isolates were confirmed to be Staphylococcus xylosus. Histopathological study showed different pathological changes caused by LPS and peptidoglycan, including shrinkage of glomeruli, congestion of blood vessels, and infiltration of inflammatory cells in the kidney, whereas dekeratinization and edematous dehydration of the epithelium were seen in the urinary bladder. Based on a comparison between histopathological changes caused by LPS and peptidoglycan, LPS induced changes of significantly higher severity than peptidoglycan.   Conclusion: Peptidoglycan of S. xylosus had remarkable histopathological effects on the renal system of mice in a dose-dependent manner. Nevertheless, these effects had less intensity than the effects of LPS of E. coli.

Article Details

Histopathological effects of S. xylosus peptidoglycan in comparison to E. coli lipopolysaccharide in the urinary tract of mice

Authors:

Harith Jabbar Fahad AL-MATHKHURY | May Talib FLAIH | Hiba Khalil TAWFIQ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1278-1285

Document Type:

Article

Publication Date:

11/2011

Document Language:

Turkish

Document ID (Org No.):

2000754378

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Lipopolysaccharides

peptidoglycan

pathogenicity

Escherichia coli

Staphylococcus xylosus

Issues

Article Details

Histopathological effects of S. xylosus peptidoglycan in comparison to E. coli lipopolysaccharide in the urinary tract of mice

Authors: Harith Jabbar Fahad AL-MATHKHURY | May Talib FLAIH | Hiba Khalil TAWFIQ

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1278-1285

Document Type: Article

Publication Date: 11/2011

Document Language: Turkish

Document ID (Org No.): 2000754378

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Lipopolysaccharides

peptidoglycan

pathogenicity

Escherichia coli

Staphylococcus xylosus

Issues

Authors:

Harith Jabbar Fahad AL-MATHKHURY | May Talib FLAIH | Hiba Khalil TAWFIQ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2012 Vol.42 Issue Sup.1, pp.1278-1285

Document Type:

Article

Publication Date:

11/2011

Document Language:

Turkish

Document ID (Org No.):

2000754378

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144