Electron Microscopic Changes of the Retina after Chronic Irradiation with Ultraviolet-A

Yasser Helmy Mohamed; Ghada M. Abdazzem; Heba Mohamed Tawfik

doi:10.12816/0005088

Article Details

Electron Microscopic Changes of the Retina after Chronic Irradiation with Ultraviolet-A

Authors:

Yasser Helmy Mohamed | Ghada M. Abdazzem | Heba Mohamed Tawfik

Publisher:

Cairo University : Faculty of Medicine

Main Topics:

Medicine

Journal:

The Egyptian Journal of Forensic Sciences and Applied Toxicology 2013 vol.13 Issue 1, pp.79-93

Document Type:

Article

Publication Date:

6/2013

Document Language:

English

Document ID (Org No.):

49506

Frequency:

Bi-Annual

pISSN:

16870875

DOI:

10.12816/0005088

Descriptors:

Keywords:

Abstract:

 Background: Fifty SKH-1 hairless mice were employed in this study. Mice were divided into two groups randomly to determine electron microscopic changes of the retina that occur after chronic use of ultraviolet irradiation.
Methods: Mice were randomly divided into two groups. Animals of the first group were exposed to ultraviolet A (UV-A) at 300KJ/m2/day for five days/week. Mice of the second group were not exposed to UV-A, and were considered as control group. After 4th and 8th months of UV-A exposure, mice were sacrificed under ether anesthesia. Retinal specimens were dissected and fixation was performed. All animal procedures were performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.
Results: After 4th month of UV-A exposure, the retinal pigmented epithelium showed large number of clear vesicles and accumulation of large number of melanosomes, while After 8th month of UV-A exposure, the retinal pigmented epithelium was lost from the central part of the retina. There was marked vesiculation of the retinal pigmented epithelium in the peripheral part of retina. Retinal pigmented epithelium showed condensation of nucleus into apoptotic body.
Conclusions: Chronic irradiation with Ultraviolet-A was associated with retinal changes. If more UV is absorbed, eye structures such as the cornea, the lens and the retina can be damaged. So the use of UV-A irradiation must be minimized to decrease these changes.

Article Details

Electron Microscopic Changes of the Retina after Chronic Irradiation with Ultraviolet-A

Authors:

Yasser Helmy Mohamed | Ghada M. Abdazzem | Heba Mohamed Tawfik

Publisher:

Cairo University : Faculty of Medicine

Main Topics:

Medicine

Journal:

The Egyptian Journal of Forensic Sciences and Applied Toxicology 2013 vol.13 Issue 1, pp.79-93

Document Type:

Article

Publication Date:

6/2013

Document Language:

English

Document ID (Org No.):

49506

Frequency:

Bi-Annual

pISSN:

16870875

DOI:

10.12816/0005088

Descriptors:

RADIATION HAZARDS

RADIATION EFFECTS

RADIATION

ULTRAVIOLET RADIATION

EYE DISEASES

RETINA

EYES

RETINITIS

EXPERIMENTS

LABORATORY EXPERIMENTS

MICE

FORENSIC MEDICINE

Electron microscope

Retina

Chronic irradiation

Ultraviolet-A

Issues

Article Details

Authors: Yasser Helmy Mohamed | Ghada M. Abdazzem | Heba Mohamed Tawfik

Publisher: Cairo University : Faculty of Medicine

Main Topics: Medicine

Journal: The Egyptian Journal of Forensic Sciences and Applied Toxicology 2013 vol.13 Issue 1, pp.79-93

Document Type: Article

Publication Date: 6/2013

Document Language: English

Document ID (Org No.): 49506

Frequency: Bi-Annual

pISSN: 16870875

DOI: 10.12816/0005088

Descriptors:

Issues

Authors:

Yasser Helmy Mohamed | Ghada M. Abdazzem | Heba Mohamed Tawfik

Publisher:

Cairo University : Faculty of Medicine

Main Topics:

Medicine

Journal:

The Egyptian Journal of Forensic Sciences and Applied Toxicology 2013 vol.13 Issue 1, pp.79-93

Document Type:

Article

Publication Date:

6/2013

Document Language:

English

Document ID (Org No.):

49506

Frequency:

Bi-Annual

pISSN:

16870875

DOI:

10.12816/0005088