Effect of Co-Treatment with Olive Leaf Extract on Cisplatin - Induced Testicular Toxicity in Rats

Naif Ibrahim Alwakid; Gomaa Mostafa-Hedeab; Haytham Aly

doi:10.12816/0045430

Article Details

Effect of Co-Treatment with Olive Leaf Extract on Cisplatin - Induced Testicular Toxicity in Rats

Authors:

Naif Ibrahim Alwakid | Gomaa Mostafa-Hedeab | Haytham Aly

Publisher:

Al Jouf University

Main Topics:

Medicine

Journal:

Aljouf University Medical Journal 2016 Vol.3 Issue 4, pp.21-29

Document Type:

Article

Publication Date:

12/2016

Document Language:

English

Document ID (Org No.):

115266

Frequency:

Quarterly

pISSN:

16586700

DOI:

10.12816/0045430

Descriptors:

Keywords:

Abstract:

 Background: Testicular toxicity, induced by anti-cancer drugs, including Cisplatin, represents a great challenge that limits its use. Ethanol olive leaf extract (OLE) is a natural product with potent antioxidant activity.
Objective: These investigations were designed to study the effect of cotreatment with OLE against testicular toxicity induced experimentally by Cisplatin.
Methods: Twenty-eight rats were classified into four groups, seven rats each: group 1 (normal control group), group 2 (received Cisplatin only for one month; 4 mg/kg body weight/twice a week), group 3 (Cisplatin + OLE 120 mg/kg/day) and group 4 (Cisplatin + OLE 160 mg/kg/day). Body weight (BW), testicular weight (TW), serum testosterone level, and testicular malondialdehyde (MDA; nM/mg protein) and reduced glutathione (GSH; nM/mg protein) contents, and catalase (mU/mg protein), superoxide dismutase (SOD; nM/mg protein) activities were measured.
Results: Cisplatin significantly decreased BW, TW, testicular catalase and SOD activities, and GSH content while increasing MDA content compared to the normal group. OLE significantly increased BW and normalized TW. The lower OLE dosage significantly increased catalase and SOD activities and GSH content compared to group Cisplatin-treated rats. The higher OLE dosage significantly improved testicular SOD activity and GSH contents only. The two OLE doses normalized the testicular MDA content and testosterone level with the restoration of the normal testicular histological structure.
Conclusion: OLE significantly attenuated cisplatin-induced testicular toxicity mostly via its anti-oxidant and anti-lipid peroxidation activities.

Article Details

Effect of Co-Treatment with Olive Leaf Extract on Cisplatin - Induced Testicular Toxicity in Rats

Authors:

Naif Ibrahim Alwakid | Gomaa Mostafa-Hedeab | Haytham Aly

Publisher:

Al Jouf University

Main Topics:

Medicine

Journal:

Aljouf University Medical Journal 2016 Vol.3 Issue 4, pp.21-29

Document Type:

Article

Publication Date:

12/2016

Document Language:

English

Document ID (Org No.):

115266

Frequency:

Quarterly

pISSN:

16586700

DOI:

10.12816/0045430

Descriptors:

OLIVES

MALE GENITAL DISEASES

TOXICITY

CATALASE

SUPEROXIDE DISMUTASE

PEPTIDES

RATS

Olive Leaf Extract

Testicular Toxicity

Catalase

Superoxide Dismutase

Cisplatin

Glutathione

Malondialdehyde

Issues

Article Details

Authors: Naif Ibrahim Alwakid | Gomaa Mostafa-Hedeab | Haytham Aly

Publisher: Al Jouf University

Main Topics: Medicine

Journal: Aljouf University Medical Journal 2016 Vol.3 Issue 4, pp.21-29

Document Type: Article

Publication Date: 12/2016

Document Language: English

Document ID (Org No.): 115266

Frequency: Quarterly

pISSN: 16586700

DOI: 10.12816/0045430

Descriptors:

Issues

Authors:

Naif Ibrahim Alwakid | Gomaa Mostafa-Hedeab | Haytham Aly

Publisher:

Al Jouf University

Main Topics:

Medicine

Journal:

Aljouf University Medical Journal 2016 Vol.3 Issue 4, pp.21-29

Document Type:

Article

Publication Date:

12/2016

Document Language:

English

Document ID (Org No.):

115266

Frequency:

Quarterly

pISSN:

16586700

DOI:

10.12816/0045430