GENETIC DIVERSITY OF CROCUS SATIVUS AND ITS CLOSE RELATIVE SPECIES ANALYZED BY iPBS-RETROTRANSPOSONS

Aysun GEDIK; Duygu ATES; Semih ERDOGMUS; Gonul COMERTPAY; Muhammed Bahattin TANYOLAC; Hakan OZKAN

doi:https://doi.org/10.17557/tjfc.357426

Article Details

GENETIC DIVERSITY OF CROCUS SATIVUS AND ITS CLOSE RELATIVE SPECIES ANALYZED BY iPBS-RETROTRANSPOSONS

Authors:

Aysun GEDIK | Duygu ATES | Semih ERDOGMUS | Gonul COMERTPAY | Muhammed Bahattin TANYOLAC | Hakan OZKAN

Publisher:

Society of Fields Crop Science

Journal:

Turkish Journal Of Field Crops 2017 Vol.22 Issue 2, pp.243-252

Document Type:

Article

Publication Date:

12/2017

Document Language:

English

Document ID (Org No.):

2000636204

Frequency:

Bi-Annual

pISSN:

1301-1111

DOI:

https://doi.org/10.17557/tjfc.357426

Abstract:

 


Saffron (Crocus L.) is a member of Crocoideae, the biggest of four subfamilies in the Iridaceae family. It has 2n = 3x = 24 chromosomes and is triploid; thus, it is sterile. In previous research, different molecular DNA markers were used but molecular characterization and genetic diversity of this complex genus have not yet been clarified. Therefore, current study aimed to determine the molecular characterization of saffron and its close relative species using inter-primer binding site (iPBS)-retrotransposon markers. Eighty-three iPBS-retrotransposon primers were used in 28 C. sativus genotypes and 17 close relative species of saffron to identify their genetic diversity. Sixteen polymorphic iPBS-retrotransposon primers generated a total of 401 polymorphic scorable bands. The mean PIC value, Nei’s genetic diversity and Shannon’s information index (I) were calculated as 0.85, 0.16 and 0.29, respectively. The results of the Unweighted Pair Group Method with Arithmetic mean UPGMA dendrogram and Principal Coordinates Analysis PCoA analysis indicated a spatial representation of the relative genetic distances among 28 saffron samples and the 17 close relative species were categorized under two distinct groups. Saffron genotypes showed very limited genetic variation and according to the iPBS-retrotransposon data, its close relatives were C. cartwrightianus and C. pallasii subsp. pallasii.

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Article Details

GENETIC DIVERSITY OF CROCUS SATIVUS AND ITS CLOSE RELATIVE SPECIES ANALYZED BY iPBS-RETROTRANSPOSONS

Authors: Aysun GEDIK | Duygu ATES | Semih ERDOGMUS | Gonul COMERTPAY | Muhammed Bahattin TANYOLAC | Hakan OZKAN

Publisher: Society of Fields Crop Science

Journal: Turkish Journal Of Field Crops 2017 Vol.22 Issue 2, pp.243-252

Document Type: Article

Publication Date: 12/2017

Document Language: English

Document ID (Org No.): 2000636204

Frequency: Bi-Annual

pISSN: 1301-1111

DOI: https://doi.org/10.17557/tjfc.357426

Issues

Authors:

Aysun GEDIK | Duygu ATES | Semih ERDOGMUS | Gonul COMERTPAY | Muhammed Bahattin TANYOLAC | Hakan OZKAN

Publisher:

Society of Fields Crop Science

Journal:

Turkish Journal Of Field Crops 2017 Vol.22 Issue 2, pp.243-252

Document Type:

Article

Publication Date:

12/2017

Document Language:

English

Document ID (Org No.):

2000636204

Frequency:

Bi-Annual

pISSN:

1301-1111

DOI:

https://doi.org/10.17557/tjfc.357426