Turkish Propolis Mitigates Oxidative Stress and Endoplasmic Reticulum Stress in Cardiac Damage Caused by Doxorubicin in Rats

Selma Cırrık; Emel Kabartan Çökeli; Gulay Hacıoğlu; Emine Gülçeri Güleç Peker

Article Details

Turkish Propolis Mitigates Oxidative Stress and Endoplasmic Reticulum Stress in Cardiac Damage Caused by Doxorubicin in Rats

Authors:

Selma Cırrık | Emel Kabartan Çökeli | Gulay Hacıoğlu | Emine Gülçeri Güleç Peker

Publisher:

Dokuz Eylul University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Journal of Basic and Clinical Health Sciences Vol.9 Issue 2, pp.299-308

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001349146

pISSN:

2564-7288

Keywords:

Abstract:

 Purpose: This study examined the effects of propolis extracted with ethanol EPE or olive oil OPE on the endoplasmic reticulum ER and oxidative stress in doxorubicin DXR-induced cardiac damage in rats. Materials and Methods: Six groups of SpragueDawley rats were used in this research: Control, EPE, OPE, DXR, EPEDXR, and OPEDXR. The extracts were administered orally for two weeks 50 mgkgday, and DXR was injected 48 hours before sacrifice 15 mgkg. Cardiac malondialdehyde MDA and glutathione GSH levels and catalase CAT activity were assayed spectrophotometrically, while cardiac glucose-regulated protein GRP-78, pro-caspase 12, and serum troponin I levels were determined via ELISA. Results: The DXR group presented elevated serum troponin I, indicating cardiac injury, increased MDA, decreased CAT activity, and reduced GSH, indicating oxidative stress, along with elevated GRP78 and decreased pro-caspase 12, indicating ER stress. Pretreatment with EPE or OPE significantly prevented DXR-induced increases in troponin I and MDA, as well as decreases in GSH, CAT activity, and pro-caspase 12, but did not significantly alter cardiac GRP78 levels. Compared with the EPEDXR group, the OPEDXR group presented higher levels of CAT activity p0.01. Conclusion: Pretreatment with EPE or OPE may provide protection against DXR-induced cardiotoxicity by suppressing both oxidative and ER stress.

 Purpose: This study examined the effects of propolis extracted with ethanol EPE or olive oil OPE on the endoplasmic reticulum ER and oxidative stress in doxorubicin DXR-induced cardiac damage in rats. Materials and Methods: Six groups of SpragueDawley rats were used in this research: Control, EPE, OPE, DXR, EPEDXR, and OPEDXR. The extracts were administered orally for two weeks 50 mgkgday, and DXR was injected 48 hours before sacrifice 15 mgkg. Cardiac malondialdehyde MDA and glutathione GSH levels and catalase CAT activity were assayed spectrophotometrically, while cardiac glucose-regulated protein GRP-78, pro-caspase 12, and serum troponin I levels were determined via ELISA. Results: The DXR group presented elevated serum troponin I, indicating cardiac injury, increased MDA, decreased CAT activity, and reduced GSH, indicating oxidative stress, along with elevated GRP78 and decreased pro-caspase 12, indicating ER stress. Pretreatment with EPE or OPE significantly prevented DXR-induced increases in troponin I and MDA, as well as decreases in GSH, CAT activity, and pro-caspase 12, but did not significantly alter cardiac GRP78 levels. Compared with the EPEDXR group, the OPEDXR group presented higher levels of CAT activity p0.01. Conclusion: Pretreatment with EPE or OPE may provide protection against DXR-induced cardiotoxicity by suppressing both oxidative and ER stress.

 Purpose: This study examined the effects of propolis extracted with ethanol EPE or olive oil OPE on the endoplasmic reticulum ER and oxidative stress in doxorubicin DXR-induced cardiac damage in rats. Materials and Methods: Six groups of SpragueDawley rats were used in this research: Control, EPE, OPE, DXR, EPEDXR, and OPEDXR. The extracts were administered orally for two weeks 50 mgkgday, and DXR was injected 48 hours before sacrifice 15 mgkg. Cardiac malondialdehyde MDA and glutathione GSH levels and catalase CAT activity were assayed spectrophotometrically, while cardiac glucose-regulated protein GRP-78, pro-caspase 12, and serum troponin I levels were determined via ELISA. Results: The DXR group presented elevated serum troponin I, indicating cardiac injury, increased MDA, decreased CAT activity, and reduced GSH, indicating oxidative stress, along with elevated GRP78 and decreased pro-caspase 12, indicating ER stress. Pretreatment with EPE or OPE significantly prevented DXR-induced increases in troponin I and MDA, as well as decreases in GSH, CAT activity, and pro-caspase 12, but did not significantly alter cardiac GRP78 levels. Compared with the EPEDXR group, the OPEDXR group presented higher levels of CAT activity p0.01. Conclusion: Pretreatment with EPE or OPE may provide protection against DXR-induced cardiotoxicity by suppressing both oxidative and ER stress.

 Purpose: This study examined the effects of propolis extracted with ethanol EPE or olive oil OPE on the endoplasmic reticulum ER and oxidative stress in doxorubicin DXR-induced cardiac damage in rats. Materials and Methods: Six groups of SpragueDawley rats were used in this research: Control, EPE, OPE, DXR, EPEDXR, and OPEDXR. The extracts were administered orally for two weeks 50 mgkgday, and DXR was injected 48 hours before sacrifice 15 mgkg. Cardiac malondialdehyde MDA and glutathione GSH levels and catalase CAT activity were assayed spectrophotometrically, while cardiac glucose-regulated protein GRP-78, pro-caspase 12, and serum troponin I levels were determined via ELISA. Results: The DXR group presented elevated serum troponin I, indicating cardiac injury, increased MDA, decreased CAT activity, and reduced GSH, indicating oxidative stress, along with elevated GRP78 and decreased pro-caspase 12, indicating ER stress. Pretreatment with EPE or OPE significantly prevented DXR-induced increases in troponin I and MDA, as well as decreases in GSH, CAT activity, and pro-caspase 12, but did not significantly alter cardiac GRP78 levels. Compared with the EPEDXR group, the OPEDXR group presented higher levels of CAT activity p0.01. Conclusion: Pretreatment with EPE or OPE may provide protection against DXR-induced cardiotoxicity by suppressing both oxidative and ER stress.

Article Details

Turkish Propolis Mitigates Oxidative Stress and Endoplasmic Reticulum Stress in Cardiac Damage Caused by Doxorubicin in Rats

Authors:

Selma Cırrık | Emel Kabartan Çökeli | Gulay Hacıoğlu | Emine Gülçeri Güleç Peker

Publisher:

Dokuz Eylul University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Journal of Basic and Clinical Health Sciences Vol.9 Issue 2, pp.299-308

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001349146

pISSN:

2564-7288

Ethanol

olive oil

GRP78

pro-caspase 12

MDA

cardiac injury

propolis

Issues

Article Details

Authors: Selma Cırrık | Emel Kabartan Çökeli | Gulay Hacıoğlu | Emine Gülçeri Güleç Peker

Publisher: Dokuz Eylul University

Main Topics: Biochemistry and Cell Biology (Other)

Journal: Journal of Basic and Clinical Health Sciences Vol.9 Issue 2, pp.299-308

Document Type: Article

Publication Date: 5/2025

Document Language: English

Document ID (Org No.): 2001349146

pISSN: 2564-7288

Issues

Authors:

Selma Cırrık | Emel Kabartan Çökeli | Gulay Hacıoğlu | Emine Gülçeri Güleç Peker

Publisher:

Dokuz Eylul University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Journal of Basic and Clinical Health Sciences Vol.9 Issue 2, pp.299-308

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001349146

pISSN:

2564-7288