Assessment of Anticancer Properties of Synthesized Pyrazole-Acridine Derivative on SH-SY5Y Human Neuroblastoma Cells

Yusuf Küçükbağrıaçık; Muna Elmusa; Fatıma Elmusa; Hümeyra Yılmaz; Rahmi Kasımoğulları; Mohammadreza Dastourı

Article Details

Assessment of Anticancer Properties of Synthesized Pyrazole-Acridine Derivative on SH-SY5Y Human Neuroblastoma Cells

Authors:

Yusuf Küçükbağrıaçık | Muna Elmusa | Fatıma Elmusa | Hümeyra Yılmaz | Rahmi Kasımoğulları | Mohammadreza Dastourı

Publisher:

Duzce University

Main Topics:

Cancer Cell Biology

Journal:

Duzce Medical Journal Vol.27 Issue 1, pp.1-10

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

2001338374

pISSN:

1307-671X

Keywords:

Abstract:

 Aim: This study aimed to synthesize a novel pyrazole acridine derivative 3-ACH and evaluate its anticancer properties on SH-SY5Y human neuroblastoma cells. Material and Methods: The pyrazole-4-carbaldehyde derivative was cyclized with dimedone and p-nitroaniline to synthesize the 3-ACH. Characterization of the compound was performed using FT-IR, NMR, HPLC-Q-TOFMS, and elemental analysis. The cytotoxic impact of the 3-ACH compound on SH-SY5Y human neuroblastoma cells was evaluated using the WST-1 assay in a dose- 50, 100, and 150 μgmL and time-dependent 12, 24, and 48 hours manner. The impact of 3-ACH on apoptosis was investigated through immunostaining for Caspase-3, -8, and -9, and BAX proteins. Results: 3-ACH reduced SH-SY5Y cell viability with the rate of 89.897.63 p0.002 at 100 μgmL concentration after 24 hours of treatment. While a higher 150 μgmL concentration showed a similar reduction 90.532.88, p0.004, the lower 50 μgmL concentration maintained high cell viability 98.371.67, p0.903 at 24 hours. All doses of 50 μgmL 94.550.65, 100 μgmL 95.181.41, and 150 μgmL 95.282.57 significantly reduced cell viability rates, at 48 hours all p0.001. Immunostaining revealed a significant upregulation in the synthesis of BAX, Caspase-3, -8, and -9 proteins in cells treated with 3-ACH 100 μgmL for 24 hours compared to the control group. Conclusion: These results indicate that 3-ACH has the potential to induce apoptosis in SH-SY5Y cells by activating both the intrinsic and extrinsic pathways, which may contribute to its cytotoxic effects. This study provides promising evidence supporting the potential of the 3-ACH compound as an anticancer therapeutic agent.

 Aim: This study aimed to synthesize a novel pyrazole acridine derivative 3-ACH and evaluate its anticancer properties on SH-SY5Y human neuroblastoma cells. Material and Methods: The pyrazole-4-carbaldehyde derivative was cyclized with dimedone and p-nitroaniline to synthesize the 3-ACH. Characterization of the compound was performed using FT-IR, NMR, HPLC-Q-TOFMS, and elemental analysis. The cytotoxic impact of the 3-ACH compound on SH-SY5Y human neuroblastoma cells was evaluated using the WST-1 assay in a dose- 50, 100, and 150 μgmL and time-dependent 12, 24, and 48 hours manner. The impact of 3-ACH on apoptosis was investigated through immunostaining for Caspase-3, -8, and -9, and BAX proteins. Results: 3-ACH reduced SH-SY5Y cell viability with the rate of 89.897.63 p0.002 at 100 μgmL concentration after 24 hours of treatment. While a higher 150 μgmL concentration showed a similar reduction 90.532.88, p0.004, the lower 50 μgmL concentration maintained high cell viability 98.371.67, p0.903 at 24 hours. All doses of 50 μgmL 94.550.65, 100 μgmL 95.181.41, and 150 μgmL 95.282.57 significantly reduced cell viability rates, at 48 hours all p0.001. Immunostaining revealed a significant upregulation in the synthesis of BAX, Caspase-3, -8, and -9 proteins in cells treated with 3-ACH 100 μgmL for 24 hours compared to the control group. Conclusion: These results indicate that 3-ACH has the potential to induce apoptosis in SH-SY5Y cells by activating both the intrinsic and extrinsic pathways, which may contribute to its cytotoxic effects. This study provides promising evidence supporting the potential of the 3-ACH compound as an anticancer therapeutic agent.

 Aim: This study aimed to synthesize a novel pyrazole acridine derivative 3-ACH and evaluate its anticancer properties on SH-SY5Y human neuroblastoma cells. Material and Methods: The pyrazole-4-carbaldehyde derivative was cyclized with dimedone and p-nitroaniline to synthesize the 3-ACH. Characterization of the compound was performed using FT-IR, NMR, HPLC-Q-TOFMS, and elemental analysis. The cytotoxic impact of the 3-ACH compound on SH-SY5Y human neuroblastoma cells was evaluated using the WST-1 assay in a dose- 50, 100, and 150 μgmL and time-dependent 12, 24, and 48 hours manner. The impact of 3-ACH on apoptosis was investigated through immunostaining for Caspase-3, -8, and -9, and BAX proteins. Results: 3-ACH reduced SH-SY5Y cell viability with the rate of 89.897.63 p0.002 at 100 μgmL concentration after 24 hours of treatment. While a higher 150 μgmL concentration showed a similar reduction 90.532.88, p0.004, the lower 50 μgmL concentration maintained high cell viability 98.371.67, p0.903 at 24 hours. All doses of 50 μgmL 94.550.65, 100 μgmL 95.181.41, and 150 μgmL 95.282.57 significantly reduced cell viability rates, at 48 hours all p0.001. Immunostaining revealed a significant upregulation in the synthesis of BAX, Caspase-3, -8, and -9 proteins in cells treated with 3-ACH 100 μgmL for 24 hours compared to the control group. Conclusion: These results indicate that 3-ACH has the potential to induce apoptosis in SH-SY5Y cells by activating both the intrinsic and extrinsic pathways, which may contribute to its cytotoxic effects. This study provides promising evidence supporting the potential of the 3-ACH compound as an anticancer therapeutic agent.

 Amaç: Bu çalışmanın amacı yeni bir pirazol akridin türevi 3-ACH sentezlemek ve bu bileşiğin SH-SY5Y insan nöroblastoma hücreleri üzerindeki antikanser özelliklerini değerlendirmektir. Gereç ve Yöntemler: Pirazol-4-karbaldehit türevi, 3-ACH sentezlemek için dimedon ve p-nitroanilin ile siklize edildi. Bileşiğin karakterizasyonu FT-IR, NMR, HPLC-Q-TOFMS ve element analizi kullanılarak yapıldı. 3-ACH bileşiğinin SH-SY5Y insan nöroblastoma hücreleri üzerindeki sitotoksik etkisi, doz 50, 100 ve 150 μgmL ve zaman 12, 24 ve 48 saat bağımlı bir şekilde WST-1 testi kullanılarak değerlendirildi. 3-ACHnin apoptoz üzerindeki etkisi, Kaspaz-3, -8, -9 ve BAX proteinleri için immün boyama yolu ile araştırıldı. Bulgular: 3-ACH, 100 μgmL konsantrasyonda 24 saatlik tedaviden sonra SH-SY5Y hücre canlılığını 89,897,63 p0,002 oranında azaltmıştır. 24 saatte, daha yüksek 150 μgmL konsantrasyon benzer bir azalma gösterirken 90,532,88, p0,004, daha düşük 50 μgmL konsantrasyon hücre canlılığını korumuştur 98,371,67, p0,903. 50 μgmL 94,550,65, 100 μgmL 95,181,41 ve 150 μgmL 95,282,57 dozlarının tümü, 48 saatte hücre canlılık oranlarını önemli ölçüde azaltmıştır tüm p0,001. İmmün boyama, 24 saat boyunca 3-ACH 100 μgmL ile muamele edilen hücrelerde, BAX, Kaspaz-3, -8 ve -9 proteinlerinin sentezinde kontrol grubuna kıyasla önemli bir artış olduğunu ortaya koymuştur. Sonuç: Bu sonuçlar, 3-ACHnin hem intrinsik hem de ekstrinsik yolakları aktive ederek SH-SY5Y hücrelerinde, sitotoksik etkilerine katkıda bulunabilecek olan, apoptozu indükleme potansiyeline sahip olduğunu göstermektedir. Bu çalışma, 3-ACH bileşiğinin antikanser terapötik ajan olarak potansiyelini destekleyen umut verici kanıtlar sunmaktadır.

Article Details

Assessment of Anticancer Properties of Synthesized Pyrazole-Acridine Derivative on SH-SY5Y Human Neuroblastoma Cells

Authors:

Yusuf Küçükbağrıaçık | Muna Elmusa | Fatıma Elmusa | Hümeyra Yılmaz | Rahmi Kasımoğulları | Mohammadreza Dastourı

Publisher:

Duzce University

Main Topics:

Cancer Cell Biology

Journal:

Duzce Medical Journal Vol.27 Issue 1, pp.1-10

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

2001338374

pISSN:

1307-671X

Chemical synthesis

antineoplastic agents

neuroblastoma

pyrazoles

acridines

cytotoxicity

apoptosisKimyasal sentez

antineoplastik ajanlar

nöroblastoma

pirazoller

akridinler

sitotoksisite

apoptoz

Issues

Article Details

Authors: Yusuf Küçükbağrıaçık | Muna Elmusa | Fatıma Elmusa | Hümeyra Yılmaz | Rahmi Kasımoğulları | Mohammadreza Dastourı

Publisher: Duzce University

Main Topics: Cancer Cell Biology

Journal: Duzce Medical Journal Vol.27 Issue 1, pp.1-10

Document Type: Article

Publication Date: 4/2025

Document Language: English

Document ID (Org No.): 2001338374

pISSN: 1307-671X

Issues

Authors:

Yusuf Küçükbağrıaçık | Muna Elmusa | Fatıma Elmusa | Hümeyra Yılmaz | Rahmi Kasımoğulları | Mohammadreza Dastourı

Publisher:

Duzce University

Main Topics:

Cancer Cell Biology

Journal:

Duzce Medical Journal Vol.27 Issue 1, pp.1-10

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

2001338374

pISSN:

1307-671X