Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)

İsmail BEZİRGANOGLU

doi:https://doi.org/10.21597/jist.851610

Article Details

Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)

Authors:

İsmail BEZİRGANOGLU

Publisher:

Igdir University

Main Topics:

Life sciences

Journal:

Journal of the Institute of Science and Technology 2021 Vol.11 Issue 2, pp.927-932

Document Type:

Article

Publication Date:

5/2021

Document Language:

English

Document ID (Org No.):

2000462762

Frequency:

Quarterly

eISSN:

2536-4618

DOI:

https://doi.org/10.21597/jist.851610

Abstract:

 

The goal of this study was to determine melatonin impacts on the embryogenic callus formation and callus necrosis recovering of alfalfa (Medicago sativa L). Calluses, obtained from leaf explants, were cultivated on Murashige and Skoog (MS) medium supplemented with 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D or 0.0125 mg mL-1 kinetin + 1 mg mL-1 melatonin. Melatonin reduced the embryogenic callus formation in both concentrations. It was detected that the weight of fresh callus in genotypes was strongly related with hormones under in vitro conditions and the lowest fresh callus weight was detected in presence of 0.0125 mg mL-1 kinetin and 1 mg mL-1 melatonin. Based on the callus necrosis recovery, the highest callus necrosis weight (0.843 g) were recorded in embryogenic calluses cultivated in 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D melatonin-supplemented medium, compared with the calluses activited with MS alone (0.587 g). In conclusion, it was detected in this study that in vitro melatonin could be used in callus necrosis recovered in tissue cultures of alfalfa.

 

The goal of this study was to determine melatonin impacts on the embryogenic callus formation and callus necrosis recovering of alfalfa (Medicago sativa L). Calluses, obtained from leaf explants, were cultivated on Murashige and Skoog (MS) medium supplemented with 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D or 0.0125 mg mL-1 kinetin + 1 mg mL-1 melatonin. Melatonin reduced the embryogenic callus formation in both concentrations. It was detected that the weight of fresh callus in genotypes was strongly related with hormones under in vitro conditions and the lowest fresh callus weight was detected in presence of 0.0125 mg mL-1 kinetin and 1 mg mL-1 melatonin. Based on the callus necrosis recovery, the highest callus necrosis weight (0.843 g) were recorded in embryogenic calluses cultivated in 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D melatonin-supplemented medium, compared with the calluses activited with MS alone (0.587 g). In conclusion, it was detected in this study that in vitro melatonin could be used in callus necrosis recovered in tissue cultures of alfalfa.

Article Details

Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)

Authors: İsmail BEZİRGANOGLU

Publisher: Igdir University

Main Topics: Life sciences

Journal: Journal of the Institute of Science and Technology 2021 Vol.11 Issue 2, pp.927-932

Document Type: Article

Publication Date: 5/2021

Document Language: English

Document ID (Org No.): 2000462762

Frequency: Quarterly

eISSN: 2536-4618

DOI: https://doi.org/10.21597/jist.851610

Issues

Authors:

İsmail BEZİRGANOGLU

Publisher:

Igdir University

Main Topics:

Life sciences

Journal:

Journal of the Institute of Science and Technology 2021 Vol.11 Issue 2, pp.927-932

Document Type:

Article

Publication Date:

5/2021

Document Language:

English

Document ID (Org No.):

2000462762

Frequency:

Quarterly

eISSN:

2536-4618

DOI:

https://doi.org/10.21597/jist.851610