Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacıhaliloğlu by means of single node culture

Hakan YILDIRIM; Ahmet ONAY; Engin TİLKAT; Zafer AKTÜRK

Article Details

Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacıhaliloğlu by means of single node culture

Authors:

Hakan YILDIRIM | Ahmet ONAY | Engin TİLKAT | Zafer AKTÜRK

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2011 Vol.35 Issue 1, pp.55-64

Document Type:

Article

Publication Date:

12/2010

Document Language:

Turkish

Document ID (Org No.):

2000808186

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Keywords:

Abstract:

 

Hacıhaliloğlu is one of the most widely cultivated apricot (Prunus armeniaca L.) cultivars in Turkey. It produces fruit of excellent quality and is consumed both fresh and dried. In this study several factors affecting in vitro culture, rapid proliferation, rooting, and acclimatization of the apricot cultivar Hacıhaliloğlu were examined. During this study significant differences in harvesting dates were observed, with the month of May being noted as the best time to examine the in vitro culture of Hacıhaliloğlu. The effects of the cytokinin 6-benzylaminopurine (BA), kinetin (Kin), and thidiazuron (TDZ) were evaluated individually, and different concentrations of BA were also tested. BA levels were tested for their effects on shoot proliferation and 1.0 mg L-1 of BA was the most suitable dose for promoting shoot multiplication cultures. The greatest number of shoots (3.42 ± 0.19) was obtained using 2.0 mg L-1 of BA; this was significantly greater than that of the control, but there was no significant difference in the mean obtained with 1 mg L-1 of BA. The effect of a carbon source on shoot proliferation was studied by using glucose, sucrose, fructose, and lactose at the 3.0% concentration. Sucrose resulted in better shoot proliferation than the other sugar varieties tested. The best rooting percentage was obtained on Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 of indole-3-butyric acid (IBA). The regenerated plants were successfully transferred to soil. Herein a simple and effective method is reported for the micropropagation of the apricot cv. Hacıhaliloğlu from adult plants.

 

Hacıhaliloğlu is one of the most widely cultivated apricot (Prunus armeniaca L.) cultivars in Turkey. It produces fruit of excellent quality and is consumed both fresh and dried. In this study several factors affecting in vitro culture, rapid proliferation, rooting, and acclimatization of the apricot cultivar Hacıhaliloğlu were examined. During this study significant differences in harvesting dates were observed, with the month of May being noted as the best time to examine the in vitro culture of Hacıhaliloğlu. The effects of the cytokinin 6-benzylaminopurine (BA), kinetin (Kin), and thidiazuron (TDZ) were evaluated individually, and different concentrations of BA were also tested. BA levels were tested for their effects on shoot proliferation and 1.0 mg L-1 of BA was the most suitable dose for promoting shoot multiplication cultures. The greatest number of shoots (3.42 ± 0.19) was obtained using 2.0 mg L-1 of BA; this was significantly greater than that of the control, but there was no significant difference in the mean obtained with 1 mg L-1 of BA. The effect of a carbon source on shoot proliferation was studied by using glucose, sucrose, fructose, and lactose at the 3.0% concentration. Sucrose resulted in better shoot proliferation than the other sugar varieties tested. The best rooting percentage was obtained on Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 of indole-3-butyric acid (IBA). The regenerated plants were successfully transferred to soil. Herein a simple and effective method is reported for the micropropagation of the apricot cv. Hacıhaliloğlu from adult plants.

Article Details

Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacıhaliloğlu by means of single node culture

Authors:

Hakan YILDIRIM | Ahmet ONAY | Engin TİLKAT | Zafer AKTÜRK

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2011 Vol.35 Issue 1, pp.55-64

Document Type:

Article

Publication Date:

12/2010

Document Language:

Turkish

Document ID (Org No.):

2000808186

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Apricot

Hacıhaliloglu

micropropagation

single node culture

Issues

Article Details

Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacıhaliloğlu by means of single node culture

Authors: Hakan YILDIRIM | Ahmet ONAY | Engin TİLKAT | Zafer AKTÜRK

Publisher: TUBITAK

Journal: Turkish Journal of Agriculture and Forestry 2011 Vol.35 Issue 1, pp.55-64

Document Type: Article

Publication Date: 12/2010

Document Language: Turkish

Document ID (Org No.): 2000808186

Frequency: Bimonthly

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Apricot

Hacıhaliloglu

micropropagation

single node culture

Issues

Authors:

Hakan YILDIRIM | Ahmet ONAY | Engin TİLKAT | Zafer AKTÜRK

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2011 Vol.35 Issue 1, pp.55-64

Document Type:

Article

Publication Date:

12/2010

Document Language:

Turkish

Document ID (Org No.):

2000808186

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X