Molecular Cloning and Expression of the Leishmania Tropica Kmp-11 Gene

Mouayad Meriee; Chadi Soukkarieh; Abdul Qader A. Abbady

doi:10.12816/0006470

Article Details

Molecular Cloning and Expression of the Leishmania Tropica Kmp-11 Gene

Authors:

Mouayad Meriee | Chadi Soukkarieh | Abdul Qader A. Abbady

Publisher:

The Egyptian Society of Parasitology

Main Topics:

Zoological sciences/Animals

Journal:

Journal of the Egyptian Society of Parasitology 2014 Vol.44 Issue 2, pp.321-328

Document Type:

Article

Publication Date:

8/2014

Document Language:

English

Document ID (Org No.):

53676

Frequency:

Tri-Annual

pISSN:

11100583

DOI:

10.12816/0006470

Descriptors:

Keywords:

Abstract:

 Kinetoplastid membrane protein-11 (KMP-11) is a small protein of 11 kDa present in all kinetoplastidprotozoa studded so far. This protein which is highly expressed in all stages ofthe Leishmania life cycle is considered a potential candidate for a leishmaniasis vaccineagainst many leishmania species. KMP-11 has been recently described in Leishmania tropica.In the present study, the KMP-11 gene was extracted from L. tropica by PCR using twooligonucleotide primers designed to amplify the entire coding region of this gene. Then, thepurified PCR products were successfully ligated into a high expression vector the pRSETGFP.This expression vector provides the opportunity to clone the desired insert as a fusionprotein with a GFP and a tag, polyhistidine region. The GFP use as a carrier to improve immuneresponse and the polyhistidine tag facilitates detection of the expressed protein withanti-His antibodies and also purification of the protein using affinity purification. Afterwards KMP-11 coding region was sequenced and the recombinant protein was induced andpurified from Escherichia coli cultures. The results of the present study will increase ourknowledge about molecular cloning and expression of the L. tropica KMP-11 gene, and thismay be used as an effective target for controlling cutenous leishmaniasis.

Article Details

Molecular Cloning and Expression of the Leishmania Tropica Kmp-11 Gene

Authors:

Mouayad Meriee | Chadi Soukkarieh | Abdul Qader A. Abbady

Publisher:

The Egyptian Society of Parasitology

Main Topics:

Zoological sciences/Animals

Journal:

Journal of the Egyptian Society of Parasitology 2014 Vol.44 Issue 2, pp.321-328

Document Type:

Article

Publication Date:

8/2014

Document Language:

English

Document ID (Org No.):

53676

Frequency:

Tri-Annual

pISSN:

11100583

DOI:

10.12816/0006470

Descriptors:

LEISHMANIA

LEISHMANIA TROPICA

PROTEINS

IMMUNITY

ANTIBODIES

Leishmania Tropica

Molecular Cloning

Expression

Issues

Article Details

Authors: Mouayad Meriee | Chadi Soukkarieh | Abdul Qader A. Abbady

Publisher: The Egyptian Society of Parasitology

Main Topics: Zoological sciences/Animals

Journal: Journal of the Egyptian Society of Parasitology 2014 Vol.44 Issue 2, pp.321-328

Document Type: Article

Publication Date: 8/2014

Document Language: English

Document ID (Org No.): 53676

Frequency: Tri-Annual

pISSN: 11100583

DOI: 10.12816/0006470

Descriptors:

Issues

Authors:

Mouayad Meriee | Chadi Soukkarieh | Abdul Qader A. Abbady

Publisher:

The Egyptian Society of Parasitology

Main Topics:

Zoological sciences/Animals

Journal:

Journal of the Egyptian Society of Parasitology 2014 Vol.44 Issue 2, pp.321-328

Document Type:

Article

Publication Date:

8/2014

Document Language:

English

Document ID (Org No.):

53676

Frequency:

Tri-Annual

pISSN:

11100583

DOI:

10.12816/0006470