Molecular Diversity and Compositional Analysis of Microbiota in Aged Refuse Biofilter Revealed by Amplicon Sequencing

Sandra C.  Anijiofor; Hasfalina  Che Man; Nik Norsyahariati  Nik Daud

doi:10.37077/25200860.2021.34.sp1.14

Article Details

Molecular Diversity and Compositional Analysis of Microbiota in Aged Refuse Biofilter Revealed by Amplicon Sequencing

Authors:

Sandra C. Anijiofor | Hasfalina Che Man | Nik Norsyahariati Nik Daud

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

metagenomics

Journal:

Basrah Journal of Agricultural Sciences Vol.34, pp.138-148

Document Type:

Article

Publication Date:

8/2021

Document Language:

English

Document ID (Org No.):

2001363593

pISSN:

2520-0860

DOI:

10.37077/25200860.2021.34.sp1.14

Keywords:

Abstract:

 The increasing significance of aged refuse biofilters for biodegradation processes in wastewater treatment necessitated the constant search for its bacterial properties. This study focused on the diversity of the bacteria present in aged refuse by DNA extraction. The PCR amplifier was used for DNA sequencing using DNA polymerase primer. For PCR amplicons purification, amplicons were extracted from 2% agarose gels and purified using DNA Gel Extraction Kit while quantification was done using QuantiFluor™ -ST. Sample libraries were pooled in equimolar and paired-end sequenced on an Illumina MiSeq platform while Raw fastq files were demultiplexed, quality-filtered using QIIME. The taxonomy of each 16S rRNA gene sequence was evaluated by RDP using confidence threshold of 0.7 while operational Units (OTUs) were clustered with 97% similarity cut-off using UPARSE. The result revealed twelve phyla clusters which include; Firmicutes, Chloroflexi, Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Planctomycetes, Nitrospirae, Cyanobacteria, Latescibacteria and Gemmatimonadetes. Furthermore, additional group classification revealed up to 513 reads of bacteria kingdom with dominant family classification of Anaerolineaceae, Ruminococcaceae, Nitrosomonadaceae, Planctomycetaceae, Clostridiaceae, Beijerinkiaceae, Xanthobacteracaea, Veillonellacaea, Cemmatimonadacaea. The presence of such diverse organisms in the aged refuse biofilter, show that the aged refuse biofilter has biodegradation potentials when treatment conditions are efficiently optimized.

Article Details

Molecular Diversity and Compositional Analysis of Microbiota in Aged Refuse Biofilter Revealed by Amplicon Sequencing

Authors:

Sandra C. Anijiofor | Hasfalina Che Man | Nik Norsyahariati Nik Daud

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

metagenomics

Journal:

Basrah Journal of Agricultural Sciences Vol.34, pp.138-148

Document Type:

Article

Publication Date:

8/2021

Document Language:

English

Document ID (Org No.):

2001363593

pISSN:

2520-0860

DOI:

10.37077/25200860.2021.34.sp1.14

Diversity

microbial community

aged refuse biofilter

Commamox

nitrification

metagenomics

Issues

Article Details

Molecular Diversity and Compositional Analysis of Microbiota in Aged Refuse Biofilter Revealed by Amplicon Sequencing

Authors: Sandra C. Anijiofor | Hasfalina Che Man | Nik Norsyahariati Nik Daud

Publisher: College of Agriculture, University of Basrah, Iraq

Main Topics: metagenomics

Journal: Basrah Journal of Agricultural Sciences Vol.34, pp.138-148

Document Type: Article

Publication Date: 8/2021

Document Language: English

Document ID (Org No.): 2001363593

pISSN: 2520-0860

DOI: 10.37077/25200860.2021.34.sp1.14

Diversity

microbial community

aged refuse biofilter

Commamox

nitrification

metagenomics

Issues

Authors:

Sandra C. Anijiofor | Hasfalina Che Man | Nik Norsyahariati Nik Daud

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

metagenomics

Journal:

Basrah Journal of Agricultural Sciences Vol.34, pp.138-148

Document Type:

Article

Publication Date:

8/2021

Document Language:

English

Document ID (Org No.):

2001363593

pISSN:

2520-0860

DOI:

10.37077/25200860.2021.34.sp1.14