Investigations on Liver Function in Mulards with Experimentally Induced Aflatoxicosis

Nely GROSEVA; İvan VALCHEV; Rumen BINEV; Dian KANAKOV; Tsanko HRISTOV; Lazarin LAZAROV; Krasimira UZUNOVA; Yordan NIKOLOV

Article Details

Investigations on Liver Function in Mulards with Experimentally Induced Aflatoxicosis

Authors:

Nely GROSEVA | İvan VALCHEV | Rumen BINEV | Dian KANAKOV | Tsanko HRISTOV | Lazarin LAZAROV | Krasimira UZUNOVA | Yordan NIKOLOV

Publisher:

İstanbul University-Cerrahpasa

Journal:

Journal of The Faculty of Veterinary Medicine Istanbul University 2014 Vol.40 Issue 1, pp.53-62

Document Type:

Article

Publication Date:

1/2014

Document Language:

Turkish

Document ID (Org No.):

2000817347

Frequency:

Tri-annual

eISSN:

2619-905X

pISSN:

2618-639X

Keywords:

Abstract:

 

Ducklings are among the most sensitive avian species to the toxic effects of aflatoxin В1 (AFB1). In this experiment, the toxic effects of AFB1 on liver morphology, blood plasma aspartate amino-transferase (AST), alanine amino-transferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), γ-glutamyltransferase (γ-GT), albumin, blood glucose, and plasma total protein (TP) were established in mulard ducks. The experiment was carried out with four groups of 20 10-day-old ducklings each. Each group included three subgroups with 10 birds. The groups were as followed: group І – control (which received standard feed according to the species and age), group ІІ – experimental, which received compound feed with 0.5 mg/kg AFB1, group ІІI– receiving compound feed supplemented with 0.8 mg/kg AFB1 and group IV – compound feed supplemented with 0.5 mg/kg AFB1 and 2g/kg Mycotox NG. The experiment lasted for 42 days. Macroscopically, livers were enlarged, rounded, with yellowish colour and a frail consistency in group ІІ and ІІI. Histopathologically, a various extent of dystrophy was detected depending on the dose of ingested toxin. The supplementation of compound feed with the mycosorbent Mycotox NG improved deviations in blood biochemical parameters and substantially reduced the severity and prevalence of histological lesions.

 


ABSTRACT

Ducklings are among the most sensitive avian species to the toxic effects of aflatoxin Ð’1 (AFB1). In this experiment, the toxic effects of AFB1 on liver morphology, blood plasma aspartate amino-transferase (AST), alanine amino-transferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), Î³-glutamyltransferase (Î³-GT), albumin, blood glucose, and plasma total protein (TP) were established in mulard ducks. The experiment was carried out with four groups of 20 10-day-old ducklings each. Each group included three subgroups with 10 birds. The groups were as followed: group Ð† – control (which received standard feed according to the species and age), group Ð†Ð† – experimental, which received compound feed with 0.5 mg/kg AFB1, group Ð†Ð†I– receiving compound feed supplemented with 0.8 mg/kg AFB1 and group IV – compound feed supplemented with 0.5 mg/kg AFB1 and 2g/kg Mycotox NG. The experiment lasted for 42 days. Macroscopically, livers were enlarged, rounded, with yellowish colour and a frail consistency in group Ð†Ð† and Ð†Ð†I. Histopathologically, a various extent of dystrophy was detected depending on the dose of ingested toxin. The supplementation of compound feed with the mycosorbent Mycotox NG improved deviations in blood biochemical parameters and substantially reduced the severity and prevalence of histological lesions.

Key Words: Aflatoxicosis, toxicity, duckling, liver damage, mycosorbent (Mycotox NG)

 

 

ÖZET

AFLATOKSİKOZİS İLE DENEYSEL OLARAK UYARILMIŞ MULARDLARDA KARACİĞER FONKSİYONLARI ÜZERİNE İNCELEMELER

Kanatlılarda aflatoksin Ð’1 (AFB1)'in toksik etkilerine en hassas olan ördek yavrularıdır. Bu çalışmada mulard ördeklerde AFB1'in karaciğer morfolojisi, plazma aspartat amino-transferaz (AST), alanin amino-transferaz (ALT), alkalin phosphataz (ALP), laktat dehidrogenaz (LDH), Î³-glutamiltransferaz (Î³-GT), albümin, kan glukoz, ve plazma total protein (TP) üzerine toksik etkileri incelenmiştir. Deney 20 adet 10 günlük yaştaki ördek yavrularından 4 grup ile gerçekleştirilmiştir. Her grupta 10 ördekten oluşan 3 alt grup bulunmaktadır. Grupların oluşturulmasında; grup Ð† – kontrol (türe ve yaşa göre standart olan başlangıç yemi verilmiştir), grup Ð†Ð† – deneysel, 0,5 mg/kg AFB1 içeren yem verilmiştir, grup Ð†Ð†I–0,8 mg/kg AFB1 içeren yem verilmiştir ve grup IV – yeme 0,5 mg/kg AFB1 ve 2 g/kg Mycotox NG katılmıştır. Deney 42 gün sürmüştür. Grup Ð†Ð† ve Ð†Ð†I'te makroskobik olarak karaciğerin büyümüş, yuvarlaklaşmış, sarı renkte ve gevrek yapıda olduğu gözlenmiştir. Histopatolojik olarak toksin alınma oranına göre değişen derecelerde distrofi tespit edilmiştir. Yeme mikosorbent Mycotox NG katılması kan biyokimyasal parametrelerde sapmaları düzeltmiş ve histolojik lezyonların şiddetini ve yaygınlığını azaltmıştır

Anahtar Kelimeler: Aflatoksikozis, toksisite, ördek yavrusu, karaciğer hasarı, mikosorbent (Mycotox NG)

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Article Details

Investigations on Liver Function in Mulards with Experimentally Induced Aflatoxicosis

Authors: Nely GROSEVA | İvan VALCHEV | Rumen BINEV | Dian KANAKOV | Tsanko HRISTOV | Lazarin LAZAROV | Krasimira UZUNOVA | Yordan NIKOLOV

Publisher: İstanbul University-Cerrahpasa

Journal: Journal of The Faculty of Veterinary Medicine Istanbul University 2014 Vol.40 Issue 1, pp.53-62

Document Type: Article

Publication Date: 1/2014

Document Language: Turkish

Document ID (Org No.): 2000817347

Frequency: Tri-annual

eISSN: 2619-905X

pISSN: 2618-639X

Aflatoksikozis

toksisite

ördek yavrusu

karaciğer hasarı

mikosorbent (Mycotox NG)

Issues

Authors:

Nely GROSEVA | İvan VALCHEV | Rumen BINEV | Dian KANAKOV | Tsanko HRISTOV | Lazarin LAZAROV | Krasimira UZUNOVA | Yordan NIKOLOV

Publisher:

İstanbul University-Cerrahpasa

Journal:

Journal of The Faculty of Veterinary Medicine Istanbul University 2014 Vol.40 Issue 1, pp.53-62

Document Type:

Article

Publication Date:

1/2014

Document Language:

Turkish

Document ID (Org No.):

2000817347

Frequency:

Tri-annual

eISSN:

2619-905X

pISSN:

2618-639X