Unveiling the Mediating Role of the REJ Region: PC1 and Extracellular Matrix Dynamics in ADPKD

Aljazi Abdullah Alrashidi; Hala Salim Sonbol

doi:10.22317/jcms.v10i1.1508

Article Details

Unveiling the Mediating Role of the REJ Region: PC1 and Extracellular Matrix Dynamics in ADPKD

Authors:

Aljazi Abdullah Alrashidi | Hala Salim Sonbol

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.10 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001379617

pISSN:

2413-0516

DOI:

10.22317/jcms.v10i1.1508

Keywords:

Abstract:

 Objective: This study aimed to investigate the interactions of PC1 with extracellular matrix proteins (ECM) to gain insights into its role in ADPKD onset. This exploration is to unravel the significance of these interactions in both the healthy development of kidneys and the underlying mechanisms of ADPKD, potentially identifying therapeutic targets.
Methods: We cloned and expressed the receptor of the egg jelly (REJ) domain as a maltose-binding protein-fusion protein (PET21-MBP(TEV)-REJ). Subsequently, we utilized this construct in a pulldown assay involving HEK 293 cells. In vitro, pull-down assays were conducted to evaluate the binding of the REJ fusion to various ECM components.
Results: The REJ fusion protein effectively binds to vitronectin, Fibulin-1, and actin filament-associated protein (AFAP) 1. These findings indicate that the REJ region acts as a mediator for the interaction between polycystin-1 and the ECM, shedding light on the functional role of polycystin-1 in both cell-matrix and cell-cell interactions.
Conclusion: The detailed characterization of REJ-ECM interactions offers a valuable foundation for future research aimed at systematically studying the effects of disease-causing mutations within the REJ module of human PC-1.

Article Details

Unveiling the Mediating Role of the REJ Region: PC1 and Extracellular Matrix Dynamics in ADPKD

Authors:

Aljazi Abdullah Alrashidi | Hala Salim Sonbol

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.10 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001379617

pISSN:

2413-0516

DOI:

10.22317/jcms.v10i1.1508

Polycystin-1

Receptor of the egg jelly (REJ) domain

Pull-down technique

MALDI-TOF MS

Extracellular matrix (ECM)

Issues

Article Details

Unveiling the Mediating Role of the REJ Region: PC1 and Extracellular Matrix Dynamics in ADPKD

Authors: Aljazi Abdullah Alrashidi | Hala Salim Sonbol

Publisher: Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal: Journal of Contemporary Medical Sciences Vol.10 Issue 1, pp.0-0

Document Type: Article

Publication Date: 2/2024

Document Language: English

Document ID (Org No.): 2001379617

pISSN: 2413-0516

DOI: 10.22317/jcms.v10i1.1508

Polycystin-1

Receptor of the egg jelly (REJ) domain

Pull-down technique

MALDI-TOF MS

Extracellular matrix (ECM)

Issues

Authors:

Aljazi Abdullah Alrashidi | Hala Salim Sonbol

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.10 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001379617

pISSN:

2413-0516

DOI:

10.22317/jcms.v10i1.1508