Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28

Ranjetta POOBATHY; Uma Rani SINNIAH; Xavier RATHINAM; Sreeramanan SUBRAMANIAM

Article Details

Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28

Authors:

Ranjetta POOBATHY | Uma Rani SINNIAH | Xavier RATHINAM | Sreeramanan SUBRAMANIAM

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.191-198

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000812489

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

The genus Dendrobium possesses horticultural importance. Dendrobium sonia-28 is an important ornamental orchid in the flower industry. Cryopreservation is a favoured long-term storage method for orchids with propagation problems. Protocorm-like bodies (PLBs) of Dendrobium sonia-28 were cryopreserved using the vitrification technique. Histology and scanning electron microscopy (SEM) observations were conducted on stock, non-cryopreserved (control), and cryopreserved PLBs of Dendrobium sonia-28 to detect cryoinjuries resulting from the vitrification protocol. Histological observations of control PLBs indicated that the preculture, osmoprotection, and dehydration steps were not physically damaging to the PLBs. Histological and SEM analyses of cryopreserved PLBs indicated that the freezing and thawing cycles inflicted damages on the parenchymatic regions of the PLBs. Only embryogenic cells survived the treatment. Scanning electron microscopy studies of the control and cryopreserved PLBs indicated that both osmotic and freezing injuries occurred only in the interior regions of the PLBs.

 

The genus Dendrobium possesses horticultural importance. Dendrobium sonia-28 is an important ornamental orchid in the flower industry. Cryopreservation is a favoured long-term storage method for orchids with propagation problems. Protocorm-like bodies (PLBs) of Dendrobium sonia-28 were cryopreserved using the vitrification technique. Histology and scanning electron microscopy (SEM) observations were conducted on stock, non-cryopreserved (control), and cryopreserved PLBs of Dendrobium sonia-28 to detect cryoinjuries resulting from the vitrification protocol. Histological observations of control PLBs indicated that the preculture, osmoprotection, and dehydration steps were not physically damaging to the PLBs. Histological and SEM analyses of cryopreserved PLBs indicated that the freezing and thawing cycles inflicted damages on the parenchymatic regions of the PLBs. Only embryogenic cells survived the treatment. Scanning electron microscopy studies of the control and cryopreserved PLBs indicated that both osmotic and freezing injuries occurred only in the interior regions of the PLBs.

Article Details

Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28

Authors:

Ranjetta POOBATHY | Uma Rani SINNIAH | Xavier RATHINAM | Sreeramanan SUBRAMANIAM

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.191-198

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000812489

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Cryopreservation

Dendrobium sonia-28

histology

protocorm-like bodies

scanning electron microscopy

vitrification

Issues

Article Details

Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28

Authors: Ranjetta POOBATHY | Uma Rani SINNIAH | Xavier RATHINAM | Sreeramanan SUBRAMANIAM

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.191-198

Document Type: Article

Publication Date: 1/2013

Document Language: Turkish

Document ID (Org No.): 2000812489

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Cryopreservation

Dendrobium sonia-28

histology

protocorm-like bodies

scanning electron microscopy

vitrification

Issues

Authors:

Ranjetta POOBATHY | Uma Rani SINNIAH | Xavier RATHINAM | Sreeramanan SUBRAMANIAM

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.191-198

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000812489

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152