The Role of Serum on the Adhesion of Cultured  Chinese Hamster Lung (CHL) Cells

Ali YILDIRIM

Article Details

The Role of Serum on the Adhesion of Cultured Chinese Hamster Lung (CHL) Cells

Authors:

Ali YILDIRIM

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 1998 Vol.28 Issue 4, pp.383-388

Document Type:

Article

Publication Date:

3/1998

Document Language:

Turkish

Document ID (Org No.):

2000758312

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

CHL Cells; Adhesion strength; serum proteins.

Abstract:

 

The roles of serum on the initial  attachment and adhesion strength of CHL  cells, which is gained following initial  attachment, spreading and growth, on tissue  culture polystyrene dish were determined by  using a convergent Microflow chamber. In the  presence of 10% fetal calf serum 98%±2%  of cells were attaching within 2 hours at  37°C. While only 74%±3% of cells were  attaching during the same period of  incubation in serum free medium. The critical  shear stresses of detachment of CHL cells  were 3.01±0.25 Nm -2 , 5.38±0.55 Nm -2 ,  7.22±0.54 Nm -2 , 9.47±0.43 Nm -2 , and  9.54±1.15 Nm -2 in the presence of 0%,  0.05%, 0.1%, 1%, and 5% fetal calf serum  respectively. In addition the effect of serum  origins on the adhesion strength of this cell  was determined. The the critical shear  stresses of detachment of CHL cells  were1.73±0.80 Nm -2 , 11.6±0.66 Nm -2 ,  and 10.96±0.73 Nm -2 in the presence of  10% of horse serum, new born calf serum,  and fetal calf serum respectively. These  results suggest that 1% serum concentration  will be sufficient for cells to gain the  maximum possible adhesion strength.

 

The roles of serum on the initial  attachment and adhesion strength of CHL  cells, which is gained following initial  attachment, spreading and growth, on tissue  culture polystyrene dish were determined by  using a convergent Microflow chamber. In the  presence of 10% fetal calf serum 98%±2%  of cells were attaching within 2 hours at  37°C. While only 74%±3% of cells were  attaching during the same period of  incubation in serum free medium. The critical  shear stresses of detachment of CHL cells  were 3.01±0.25 Nm -2 , 5.38±0.55 Nm -2 ,  7.22±0.54 Nm -2 , 9.47±0.43 Nm -2 , and  9.54±1.15 Nm -2 in the presence of 0%,  0.05%, 0.1%, 1%, and 5% fetal calf serum  respectively. In addition the effect of serum  origins on the adhesion strength of this cell  was determined. The the critical shear  stresses of detachment of CHL cells  were1.73±0.80 Nm -2 , 11.6±0.66 Nm -2 ,  and 10.96±0.73 Nm -2 in the presence of  10% of horse serum, new born calf serum,  and fetal calf serum respectively. These  results suggest that 1% serum concentration  will be sufficient for cells to gain the  maximum possible adhesion strength.

Article Details

The Role of Serum on the Adhesion of Cultured Chinese Hamster Lung (CHL) Cells

Authors: Ali YILDIRIM

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 1998 Vol.28 Issue 4, pp.383-388

Document Type: Article

Publication Date: 3/1998

Document Language: Turkish

Document ID (Org No.): 2000758312

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

CHL Cells; Adhesion strength; serum proteins.

Issues

Authors:

Ali YILDIRIM

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 1998 Vol.28 Issue 4, pp.383-388

Document Type:

Article

Publication Date:

3/1998

Document Language:

Turkish

Document ID (Org No.):

2000758312

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144