SYNTHESIS OF MEDICINAL LEECH (HİRUDO VERBANA) SALIVA-INCORPORATED HYBRID NANOFLOWERS AND EVALUATIONS OF THEIR BIOLOGICAL APPLICATIONS

Erdal Yılmaz; Fatih Doğan Koca; Ayşe Demirbaş; Izzet Burcin Saticioglu; Behzat Çimen; İsmail Öçsoy

Article Details

SYNTHESIS OF MEDICINAL LEECH (HİRUDO VERBANA) SALIVA-INCORPORATED HYBRID NANOFLOWERS AND EVALUATIONS OF THEIR BIOLOGICAL APPLICATIONS

Authors:

Erdal Yılmaz | Fatih Doğan Koca | Ayşe Demirbaş | Izzet Burcin Saticioglu | Behzat Çimen | İsmail Öçsoy

Publisher:

Yozgat Bozok University

Main Topics:

Veteriner Mikrobiyolojisi

Journal:

Bozok Veterinary Sciences Vol.6 Issue 1, pp.35-40

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001350612

pISSN:

2757-5179

Keywords:

Abstract:

 Herein, we reported the formation of medicinal leech saliva LS-incorporated organic-inorganic nanoflower LS-NF with peroxidase mimic and antimicrobial activities. The medicinal leech saliva was used as an organic component and copper ion Cu2 acted as an inorganic part in the synthesis of LS-NF. Leech saliva was obtained from medicinal leeches Hirudo verbana, contains various amino acids, and it can react with Cu2 ions in phosphate buffer through its accessible amine groups to form flower-shaped structures. These LS-NFs showed quite uniform and monodispersed morphology. They were systematically characterized with SEM, FTIR, and XRD for evaluation of morphology, bond vibrations, and crystal structure, respectively. The LS-NF acted as a Fenton reagent in the presence of hydrogen peroxide H2O2, then we investigated their peroxidase-like and antimicrobial activities through the Fenton reaction.

 Herein, we reported the formation of medicinal leech saliva LS-incorporated organic-inorganic nanoflower LS-NF with peroxidase mimic and antimicrobial activities. The medicinal leech saliva was used as an organic component and copper ion Cu2 acted as an inorganic part in the synthesis of LS-NF. Leech saliva was obtained from medicinal leeches Hirudo verbana, contains various amino acids, and it can react with Cu2 ions in phosphate buffer through its accessible amine groups to form flower-shaped structures. These LS-NFs showed quite uniform and monodispersed morphology. They were systematically characterized with SEM, FTIR, and XRD for evaluation of morphology, bond vibrations, and crystal structure, respectively. The LS-NF acted as a Fenton reagent in the presence of hydrogen peroxide H2O2, then we investigated their peroxidase-like and antimicrobial activities through the Fenton reaction.

 Herein, we reported the formation of medicinal leech saliva LS-incorporated organic-inorganic nanoflower LS-NF with peroxidase mimic and antimicrobial activities. The medicinal leech saliva was used as an organic component and copper ion Cu2 acted as an inorganic part in the synthesis of LS-NF. Leech saliva was obtained from medicinal leeches Hirudo verbana, contains various amino acids, and it can react with Cu2 ions in phosphate buffer through its accessible amine groups to form flower-shaped structures. These LS-NFs showed quite uniform and monodispersed morphology. They were systematically characterized with SEM, FTIR, and XRD for evaluation of morphology, bond vibrations, and crystal structure, respectively. The LS-NF acted as a Fenton reagent in the presence of hydrogen peroxide H2O2, then we investigated their peroxidase-like and antimicrobial activities through the Fenton reaction.

 Bu çalışmada, tıbbi sülük salyası H. verbana kullanarak organik ve inorganik çiçek şekilli nanoyapıların LS-NF peroksidaz ve antimikrobiyal etkilere sahip olduğu bildirilmiştir. Çiçek şekilli nano yapıların sentesinde sülük salyası organik bileşen olarak kullanılırken, bakır iyonları Cu2 ise inorganik bileşen olarak rol almıştır. Birçok amino asit içeren tıbbi sülük salyası fosfat tampon içindeki Cu2 iyonlarının amin grupları üzerinden çiçek şekilli nano yapılar oluşturmaktadır. Bu çiçek şekilli nano yapılar oldukça düzgün ve homojen bir dağılım sergiledi. Sentezlenen bu nano yapılar morfolojik, bağ titreşimleri ve kristal yapıları bakımından SEM, FTIR ve XDR analizleri ile sistematik bir şekilde karakterize edildi. Çiçek şekilli nano yapılar LS-NF hidrojen peroksit H2O2 varlığında bir Fenton reaktifi olarak davranmış olup, bunlar peroksidaz benzeri ve antibiyotik aktiviteler bakımından incelendi.

Article Details

SYNTHESIS OF MEDICINAL LEECH (HİRUDO VERBANA) SALIVA-INCORPORATED HYBRID NANOFLOWERS AND EVALUATIONS OF THEIR BIOLOGICAL APPLICATIONS

Authors:

Erdal Yılmaz | Fatih Doğan Koca | Ayşe Demirbaş | Izzet Burcin Saticioglu | Behzat Çimen | İsmail Öçsoy

Publisher:

Yozgat Bozok University

Main Topics:

Veteriner Mikrobiyolojisi

Journal:

Bozok Veterinary Sciences Vol.6 Issue 1, pp.35-40

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001350612

pISSN:

2757-5179

Medicinal leech saliva

hybrid nanoflower,

fenton reaction

peroxidase-like activity

antimicrobial activitiesTıbbi sülük salyası

hibrid nanoçiçek

Fenton reaksiyonu

Issues

Article Details

Authors: Erdal Yılmaz | Fatih Doğan Koca | Ayşe Demirbaş | Izzet Burcin Saticioglu | Behzat Çimen | İsmail Öçsoy

Publisher: Yozgat Bozok University

Main Topics: Veteriner Mikrobiyolojisi

Journal: Bozok Veterinary Sciences Vol.6 Issue 1, pp.35-40

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001350612

pISSN: 2757-5179

Issues

Authors:

Erdal Yılmaz | Fatih Doğan Koca | Ayşe Demirbaş | Izzet Burcin Saticioglu | Behzat Çimen | İsmail Öçsoy

Publisher:

Yozgat Bozok University

Main Topics:

Veteriner Mikrobiyolojisi

Journal:

Bozok Veterinary Sciences Vol.6 Issue 1, pp.35-40

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001350612

pISSN:

2757-5179