Homocysteine Influx and Efflux: Participation of Erythrocytes in Homocysteine Homeostasis of the Plasma

Deniz YILDIZ; Züleyha ÇİVİ; Zuleyha CIVI; Yeliz ÇAKIR

Article Details

Homocysteine Influx and Efflux: Participation of Erythrocytes in Homocysteine Homeostasis of the Plasma

Authors:

Deniz YILDIZ | Züleyha ÇİVİ | Zuleyha CIVI | Yeliz ÇAKIR

Publisher:

Gazi University

Journal:

Gazi University Journal of Science 2010 Vol.23 Issue 3, pp.249-253

Document Type:

Article

Publication Date:

8/2010

Document Language:

English

Document ID (Org No.):

2000429781

eISSN:

2147-1762

Abstract:

 


ABSTRACT

One of the main objective of the present study was to determine if erythrocytes play a role in regulation of plasma homocysteine concentration. Another objective was to investigate if erythrocytes convert homocystine into homocysteine. In the present study, we exposed erytrocytes to different concentrations of homocysteine and then measured the nonprotein sulfhydryl (NPSH) concentrations. Erythrocytes treated in the same manner were later utilized for the homocysteine efflux studies. The effect of temperature on the influx and the efflux processes were also evaluated. We also determined the rate of homocysteine influx in the presence of different amino acids. The homocysteine influx studies demonstrated that erythrocytes can respond to increases in homocysteine concentration in the extracellular media and influx homocysteine in a concentration-dependent manner. NPSH concentrations in erythrocytes treated with 1 mM homocysteine reached to 1.47 ï½± 0.01 ìmol/ml erythrocyte in 1 h whereas this concentration reached to 2.01 ï½± 0.1 ìmol/ml erythrocyte in 3 mM homocysteine treated erytrocytes. The homocysteine efflux is also determined to be time-and concentration -dependent. Extracellular concentration of NPSH in 1 mM homocysteine pre-treated erythrocytes reached to 0.266 ï½±  0.02 ìmol/ml erythrocyte in 1 h whereas this concentration reached to 0.64 ï½± 0.01 ìmol/ml erythrocyte with 3 mM homocysteine pre-treated erythrocytes. Our results also indicate that erythrocytes convert extracellulary applied homocystine into homocysteine. Depending on our results, it could be concluded that eryhtrocytes may play a significant role in the regulation of plasma homocysteine homeostasis.

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Article Details

Homocysteine Influx and Efflux: Participation of Erythrocytes in Homocysteine Homeostasis of the Plasma

Authors: Deniz YILDIZ | Züleyha ÇİVİ | Zuleyha CIVI | Yeliz ÇAKIR

Publisher: Gazi University

Journal: Gazi University Journal of Science 2010 Vol.23 Issue 3, pp.249-253

Document Type: Article

Publication Date: 8/2010

Document Language: English

Document ID (Org No.): 2000429781

eISSN: 2147-1762

Issues

Authors:

Deniz YILDIZ | Züleyha ÇİVİ | Zuleyha CIVI | Yeliz ÇAKIR

Publisher:

Gazi University

Journal:

Gazi University Journal of Science 2010 Vol.23 Issue 3, pp.249-253

Document Type:

Article

Publication Date:

8/2010

Document Language:

English

Document ID (Org No.):

2000429781

eISSN:

2147-1762