Anti-HDV enzyme immunoassay index in predicting HDV viremia in serum samples

Sibel Özsu CAYMAZ; Ayça Arzu SAYINER; Ayşe Aydan ÖZKÜTÜK; Yusuf Hakan ABACIOĞLU

Article Details

Anti-HDV enzyme immunoassay index in predicting HDV viremia in serum samples

Authors:

Sibel Özsu CAYMAZ | Ayça Arzu SAYINER | Ayşe Aydan ÖZKÜTÜK | Yusuf Hakan ABACIOĞLU

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.294-298

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000753814

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

To determine whether the total anti-delta antibody enzyme immunoassay (EIA) test results can be used to predict viremia.   Materials and methods: The EIA index value of an anti-delta total antibody assay was determined in 76 serum samples obtained from 67 patients positive for the anti-hepatitis delta virus (HDV) antibody. The presence of HDV RNA was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). The relation between the RT-PCR result and EIA index was evaluated by ROC analysis.   Results: Of the 76 total samples, 56 (74%) anti-HDV-positive samples were found to be PCR-positive. The samples with EIA index values below 75.75 were PCR-negative, while samples with index values above 102.55 were PCR-positive. When HDV RNA presence is considered as the gold standard, the sensitivity, specificity, and positive and negative predictive values of the chosen EIA index (100.10) in predicting viremia were 93%, 80%, 93%, and 76%, respectively.   Conclusion: HDV RNA is the most reliable marker for the diagnosis of active HDV infection, and EIA index value is closely related to viral RNA presence.

 

To determine whether the total anti-delta antibody enzyme immunoassay (EIA) test results can be used to predict viremia.   Materials and methods: The EIA index value of an anti-delta total antibody assay was determined in 76 serum samples obtained from 67 patients positive for the anti-hepatitis delta virus (HDV) antibody. The presence of HDV RNA was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). The relation between the RT-PCR result and EIA index was evaluated by ROC analysis.   Results: Of the 76 total samples, 56 (74%) anti-HDV-positive samples were found to be PCR-positive. The samples with EIA index values below 75.75 were PCR-negative, while samples with index values above 102.55 were PCR-positive. When HDV RNA presence is considered as the gold standard, the sensitivity, specificity, and positive and negative predictive values of the chosen EIA index (100.10) in predicting viremia were 93%, 80%, 93%, and 76%, respectively.   Conclusion: HDV RNA is the most reliable marker for the diagnosis of active HDV infection, and EIA index value is closely related to viral RNA presence.

Article Details

Anti-HDV enzyme immunoassay index in predicting HDV viremia in serum samples

Authors:

Sibel Özsu CAYMAZ | Ayça Arzu SAYINER | Ayşe Aydan ÖZKÜTÜK | Yusuf Hakan ABACIOĞLU

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.294-298

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000753814

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Anti-HDV enzyme immunoassay

ROC analysis

HDV RNA

reverse transcriptase polymerase chain reaction

Issues

Article Details

Anti-HDV enzyme immunoassay index in predicting HDV viremia in serum samples

Authors: Sibel Özsu CAYMAZ | Ayça Arzu SAYINER | Ayşe Aydan ÖZKÜTÜK | Yusuf Hakan ABACIOĞLU

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.294-298

Document Type: Article

Publication Date: 1/2013

Document Language: Turkish

Document ID (Org No.): 2000753814

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Anti-HDV enzyme immunoassay

ROC analysis

HDV RNA

reverse transcriptase polymerase chain reaction

Issues

Authors:

Sibel Özsu CAYMAZ | Ayça Arzu SAYINER | Ayşe Aydan ÖZKÜTÜK | Yusuf Hakan ABACIOĞLU

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 2, pp.294-298

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000753814

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144