Genotype distribution of high-risk HPVs in cervical cytology samples examined in the pathology laboratory of Konya City Hospital and its relationship with epithelial cell anomalies

İlknur Küçükosmanoğlu

Article Details

Genotype distribution of high-risk HPVs in cervical cytology samples examined in the pathology laboratory of Konya City Hospital and its relationship with epithelial cell anomalies

Authors:

İlknur Küçükosmanoğlu

Publisher:

Selcuk University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Selçuk Sağlık Dergisi Vol.4 Issue Kongre Özel, pp.18-30

Document Type:

Article

Publication Date:

9/2023

Document Language:

Turkish

Document ID (Org No.):

2001298740

pISSN:

2717-8250

Keywords:

Abstract:

 Introduction: Cervical cancer is one of the most common cancers in women. Persistent infection with high-risk human papillomavirus hrHPV is the main etiologic factor for cervical cancer. Cervical cytology and HPV DNA genotyping are used as screening tests for cervical neoplasms. In this study, it was aimed to determine the intraepithelial lesion and malignancy ILM rate and high-risk HPV DNA genotype distribution of cases in Konya province, whose cervical cytology samples were taken and HPV DNA genotype analysis was performed simultaneously. Material and method: The study included 145 cases with positive hrHPV DNA results out of 3885 cases who underwent cervical cytology and simultaneous HPV DNA genotype analysis in Konya City Hospital Medical Pathology Department between January 2021 and December 2021. The data of the cases were obtained from the pathology archive. Liquid-based cytology materials stained with Papanicolaou stain PAP were re-evaluated and classified according to the Bethesda classification. Results: hrHPV positivity was detected in 3.7 of 3885 cases. The mean age of the patients was 41.99.7. The most common HPV type in patients was determined as other hrHPV DNA genotype 31,33,35,39,45,51,52.5 6,58,59,66,68 60. Again, in most of the HPV-positive cases 52.4, the cytological diagnosis is negative for ILM. Among the cytological diagnoses in HPV-positive cases, the most frequently observed lesion is atypical squamous cells of undetermined significance ASCUS 49.3. When cytological diagnosis distributions were evaluated according to HPV types, there was no statistically significant difference between patients with and without intraepithelial lesions according to HPV types. The distribution of ILM-positive cytopathological diagnoses by HPV types did not differ statistically significantly. The distribution of all diagnoses, including ILM-negative cytopathological diagnoses, according to HPV types did not show a statistically significant difference. Conclusion: In studies conducted in Turkey, the prevalence of HPV is 2-6. In a study conducted in the Konya region about 5 years ago, the prevalence of HPV was found to be 3.16. According to our results, there is a slight increase in the rate. In our study, contrary to the literature, the most common type was not HPV16, but other types of hrHPV DNA genotype 31,33,35,39,45,51,52,5 6,58,59,66,68.HPV prevalence and genotypes countries vary between countries and even in different regions of the same country. Larger epidemiological studies are needed in different regions of our country to accurately assess the prevalence of HPV infection.

 Introduction: Cervical cancer is one of the most common cancers in women. Persistent infection with high-risk human papillomavirus hrHPV is the main etiologic factor for cervical cancer. Cervical cytology and HPV DNA genotyping are used as screening tests for cervical neoplasms. In this study, it was aimed to determine the intraepithelial lesion and malignancy ILM rate and high-risk HPV DNA genotype distribution of cases in Konya province, whose cervical cytology samples were taken and HPV DNA genotype analysis was performed simultaneously. Material and method: The study included 145 cases with positive hrHPV DNA results out of 3885 cases who underwent cervical cytology and simultaneous HPV DNA genotype analysis in Konya City Hospital Medical Pathology Department between January 2021 and December 2021. The data of the cases were obtained from the pathology archive. Liquid-based cytology materials stained with Papanicolaou stain PAP were re-evaluated and classified according to the Bethesda classification. Results: hrHPV positivity was detected in 3.7 of 3885 cases. The mean age of the patients was 41.99.7. The most common HPV type in patients was determined as other hrHPV DNA genotype 31,33,35,39,45,51,52.5 6,58,59,66,68 60. Again, in most of the HPV-positive cases 52.4, the cytological diagnosis is negative for ILM. Among the cytological diagnoses in HPV-positive cases, the most frequently observed lesion is atypical squamous cells of undetermined significance ASCUS 49.3. When cytological diagnosis distributions were evaluated according to HPV types, there was no statistically significant difference between patients with and without intraepithelial lesions according to HPV types. The distribution of ILM-positive cytopathological diagnoses by HPV types did not differ statistically significantly. The distribution of all diagnoses, including ILM-negative cytopathological diagnoses, according to HPV types did not show a statistically significant difference. Conclusion: In studies conducted in Turkey, the prevalence of HPV is 2-6. In a study conducted in the Konya region about 5 years ago, the prevalence of HPV was found to be 3.16. According to our results, there is a slight increase in the rate. In our study, contrary to the literature, the most common type was not HPV16, but other types of hrHPV DNA genotype 31,33,35,39,45,51,52,5 6,58,59,66,68.HPV prevalence and genotypes countries vary between countries and even in different regions of the same country. Larger epidemiological studies are needed in different regions of our country to accurately assess the prevalence of HPV infection.

 Introduction: Cervical cancer is one of the most common cancers in women. Persistent infection with high-risk human papillomavirus hrHPV is the main etiologic factor for cervical cancer. Cervical cytology and HPV DNA genotyping are used as screening tests for cervical neoplasms. In this study, it was aimed to determine the intraepithelial lesion and malignancy ILM rate and high-risk HPV DNA genotype distribution of cases in Konya province, whose cervical cytology samples were taken and HPV DNA genotype analysis was performed simultaneously. Material and method: The study included 145 cases with positive hrHPV DNA results out of 3885 cases who underwent cervical cytology and simultaneous HPV DNA genotype analysis in Konya City Hospital Medical Pathology Department between January 2021 and December 2021. The data of the cases were obtained from the pathology archive. Liquid-based cytology materials stained with Papanicolaou stain PAP were re-evaluated and classified according to the Bethesda classification. Results: hrHPV positivity was detected in 3.7 of 3885 cases. The mean age of the patients was 41.99.7. The most common HPV type in patients was determined as other hrHPV DNA genotype 31,33,35,39,45,51,52.5 6,58,59,66,68 60. Again, in most of the HPV-positive cases 52.4, the cytological diagnosis is negative for ILM. Among the cytological diagnoses in HPV-positive cases, the most frequently observed lesion is atypical squamous cells of undetermined significance ASCUS 49.3. When cytological diagnosis distributions were evaluated according to HPV types, there was no statistically significant difference between patients with and without intraepithelial lesions according to HPV types. The distribution of ILM-positive cytopathological diagnoses by HPV types did not differ statistically significantly. The distribution of all diagnoses, including ILM-negative cytopathological diagnoses, according to HPV types did not show a statistically significant difference. Conclusion: In studies conducted in Turkey, the prevalence of HPV is 2-6. In a study conducted in the Konya region about 5 years ago, the prevalence of HPV was found to be 3.16. According to our results, there is a slight increase in the rate. In our study, contrary to the literature, the most common type was not HPV16, but other types of hrHPV DNA genotype 31,33,35,39,45,51,52,5 6,58,59,66,68.HPV prevalence and genotypes countries vary between countries and even in different regions of the same country. Larger epidemiological studies are needed in different regions of our country to accurately assess the prevalence of HPV infection.

 Giriş: Serviks kanseri, kadınlarda en sık görülen kanserlerden biridir. Yüksek riskli insan papilloma virüsü yrHPV ile kalıcı enfeksiyon, serviks kanseri için ana etiyolojik faktördür. Servikal sitoloji ve HPV DNA genotiplendirmesi, servikal neoplaziler için tarama testi olarak kullanılmaktadır. Bu çalışmada, Konya ilindeki, servikal sitoloji örneği alınmış ve eş zamanlı HPV DNA genotip analizi yapılmış olguların intraepitelyal lezyon ve malignite ILM oranını ve yüksek riskli HPV DNA genotip dağılımını saptamak amaçlanmıştır. Materyal ve metot: Çalışmaya ocak 2021-aralık 2021 tarihleri arasında Konya Şehir hastanesi Tıbbi Patoloji Bölümünde servikal sitoloji ve eş zamanlı HPV DNA genotip analizi yapılan 3885 olgudan yrHPV DNA sonucu pozitif olan 145 olgu dahil edildi. Olguların verilerine patoloji arşivinden ulaşıldı. Papanicolaou boyasıPAP ile boyanmış sıvı bazlı sitoloji materyalleri, tekrar değerlendirilmeye alındı, Bethesda sınıflamasına göre sınıflandırıldı. Bulgular: 3885 olgunun 3,7sinde yrHPV pozitifliği saptandı. Hastaların yaş ortalaması 41.99.7 dir. Hastalarda en çok görülen HPV tipi, diğer yrHPV DNA genotip 31,33,35,39,45,51,52,5 6,58,59,66,68 olarak belirlenmiştir 60. Yine HPV pozitif olguların çoğunda 52.4 sitolojik tanı ILM açısından negatiftir. HPV pozitif olgularda sitolojik tanılar arasında ise en sık gözlenen lezyon önemi belirlenemeyen atipik skuamöz hücrelerASCUSdir 49,3. HPV tiplerine göre sitolojik tanı dağılımları değerlendirildiğinde HPV tiplerine göre intraepitelyal lezyon gözlenen ve gözlenmeyen hastaların dağılımı, HPV tiplerine göre negatif sitopatolojik tanılar dahil tüm tanıların dağılımı ve HPV tiplerine göre sadece pozitif sitopatolojik tanıların dağılımları istatistiksel açıdan anlamlı farklılık göstermemiştir. Tartışma ve Sonuç: Türkiyede yapılan çalışmalarda yrHPV prevalansı 2-6dır. Konya bölgesinde yaklaşık 5 yıl önce yapılan bir çalışmada yrHPV prevalansı 3,16 olarak bulunmuştur. Bizim sonuçlarımıza göre oranda hafif bir artış mevcuttur. Bizim çalışmamızda, literatürün aksine, en yaygın tip, HPV16 değil, diğer yrHPV DNA genotip 31,33,35,39,45,51,52,5 6,58,59,66,68 tipleridir. HPV prevalansı ve genotipler ülkeler arasında ve hatta aynı ülkenin farklı bölgelerinde farklılıklar göstermektedir. HPV enfeksiyonunun doğru prevalansını değerlendirmek için ülkemizin farklı bölgelerinde daha geniş epidemiyolojik çalışmalara ihtiyaç vardır.

Article Details

Genotype distribution of high-risk HPVs in cervical cytology samples examined in the pathology laboratory of Konya City Hospital and its relationship with epithelial cell anomalies

Authors:

İlknur Küçükosmanoğlu

Publisher:

Selcuk University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Selçuk Sağlık Dergisi Vol.4 Issue Kongre Özel, pp.18-30

Document Type:

Article

Publication Date:

9/2023

Document Language:

Turkish

Document ID (Org No.):

2001298740

pISSN:

2717-8250

HPV DNA

HPV DNA

intraepithelial lesion

cervical cytology

intraepithelial lesion;cervical cytologyHPV DNA

HPV DNA

intraepitelyal lezyon

servikal sitoloji

HPV DNA; , intraepitelyal lezyon; servikal sitoloji

intraepithelial lesion

cervical cytology

Issues

Article Details

Authors: İlknur Küçükosmanoğlu

Publisher: Selcuk University

Main Topics: Klinik Tıp Bilimleri

Journal: Selçuk Sağlık Dergisi Vol.4 Issue Kongre Özel, pp.18-30

Document Type: Article

Publication Date: 9/2023

Document Language: Turkish

Document ID (Org No.): 2001298740

pISSN: 2717-8250

Issues

Authors:

İlknur Küçükosmanoğlu

Publisher:

Selcuk University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Selçuk Sağlık Dergisi Vol.4 Issue Kongre Özel, pp.18-30

Document Type:

Article

Publication Date:

9/2023

Document Language:

Turkish

Document ID (Org No.):

2001298740

pISSN:

2717-8250