Establishment of Callus Cultures of Gentiana olivieri Griseb. and Investigation of Secondary Metabolites

Canan YAĞCI TÜZÜN; Mehmet TOKER; Gülnur TOKER

doi:https://doi.org/10.1501/Tarimbil_0000001241

Article Details

Establishment of Callus Cultures of Gentiana olivieri Griseb. and Investigation of Secondary Metabolites

Authors:

Canan YAĞCI TÜZÜN | Mehmet TOKER | Gülnur TOKER

Publisher:

Ankara University

Journal:

Journal of Agricultural Sciences 2013 Vol.19 Issue 3, pp.163-169

Document Type:

Article

Publication Date:

9/2013

Document Language:

English

Document ID (Org No.):

2000608569

Frequency:

Quarterly

eISSN:

2148-9297

DOI:

https://doi.org/10.1501/Tarimbil_0000001241

Abstract:

 


Flowered herba of Gentiana olivieri which is known as Afat in Gaziantep region is used in traditional folk medicine as appetizer, digestive aid, antidiabetic, antianemic, antihepatotoxic and antidepressant. In this study, callus production capacity of root and leaf explants obtained from in vitro germinated seeds of G. olivieri were investigated in dark. WPM or MS medium containing 0.5 mg L-1 BAP - 1 mg L-1 NAA or 0.2 and 0.5 mg L-1 Kinetin-1 mg L-1 NAA were used for stimulating the induction of callus tissue. The calli were subcultured every 4 weeks. Callus growth index was calculated for determination of increasing the callus quantity. The best callus growths were observed in leaf explants formed on WPM containing 0.5 mg L-1 BAP - 1 mg L-1 NAA after 4 weeks followed by explants sowing and in root explants formed on MS medium containing 0.2 mg L-1 Kinetin - 1 mg L-1 NAA after 8 weeks of culture. Calli which were induced on leaf explants grown on WPM during three subcultures were extracted with methanol. Secondary metabolites were investigated by thin layer chromatography (TLC). Spots, which were expected to have iridoidal structure, were observed on TLC plates.

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Gaziantep yöresinde Afat olarak bilinen Gentiana olivieri’nin herbası, halk arasında iştah açıcı, sindirime yardımcı, antidiyabetik, antianemik, antihepatotoksik ve sakinleştirici olarak kullanılmaktadır. Bu çalışmada, in vitro çimlendirilen G. olivieri’den alınan kök ve yaprak eksplantlarının karanlıkta kallus üretim kapasitesi araştırıldı. WPM ve MS ortamlarına kallus dokusu oluşumunu uyarmak için Naftalen Asetik Asit (NAA) (1 mg/l)’ya ek olarak Benzil Amino Purin (BAP) (0.5 mg/l) veya Kinetin (0.2 and 0.5 mg/l) eklendi. Kalluslar her 4 haftada bir taze ortama aktarıldı. Kallus miktarındaki artışı belirlemek için kallus büyüme indeksi hesaplandı. En iyi kallus gelişimi yaprak eksplantında, 1 mg/l NAA ve 0.5 mg/l BAP içeren WPM’de, eksplant ekimini takiben 30. günün sonunda, kök eksplantında ise 1 mg/l NAA ve 0.2 mg/l Kinetin içeren MS ortamında, 60. gün sonunda gözlendi. 

 

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Article Details

Establishment of Callus Cultures of Gentiana olivieri Griseb. and Investigation of Secondary Metabolites

Authors: Canan YAĞCI TÜZÜN | Mehmet TOKER | Gülnur TOKER

Publisher: Ankara University

Journal: Journal of Agricultural Sciences 2013 Vol.19 Issue 3, pp.163-169

Document Type: Article

Publication Date: 9/2013

Document Language: English

Document ID (Org No.): 2000608569

Frequency: Quarterly

eISSN: 2148-9297

DOI: https://doi.org/10.1501/Tarimbil_0000001241

Issues

Authors:

Canan YAĞCI TÜZÜN | Mehmet TOKER | Gülnur TOKER

Publisher:

Ankara University

Journal:

Journal of Agricultural Sciences 2013 Vol.19 Issue 3, pp.163-169

Document Type:

Article

Publication Date:

9/2013

Document Language:

English

Document ID (Org No.):

2000608569

Frequency:

Quarterly

eISSN:

2148-9297

DOI:

https://doi.org/10.1501/Tarimbil_0000001241